VIRA_AGRFC
ID VIRA_AGRFC Reviewed; 833 AA.
AC P18540; Q52297;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Wide host range VirA protein;
DE Short=WHR VirA;
DE EC=2.7.13.3;
GN Name=virA; OrderedLocusNames=Atu6166; ORFNames=AGR_pTi_2;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2796735; DOI=10.1111/j.1365-2958.1989.tb00274.x;
RA Morel P., Powell B.S., Rogowsky P.M., Kado C.I.;
RT "Characterization of the virA virulence gene of the nopaline plasmid,
RT pTiC58, of Agrobacterium tumefaciens.";
RL Mol. Microbiol. 3:1237-1246(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Powell G.K.;
RL Submitted (NOV-1995) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Activates VirG, by phosphorylating it, in the presence of
CC acetosyringone or hydroxysyringone.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; J03320; AAA91590.1; -; Genomic_DNA.
DR EMBL; L48210; AAA79282.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90927.1; -; Genomic_DNA.
DR PIR; AD3248; AD3248.
DR PIR; S06972; S06972.
DR RefSeq; NP_396486.1; NC_003065.3.
DR RefSeq; WP_010974914.1; NC_003065.3.
DR AlphaFoldDB; P18540; -.
DR SMR; P18540; -.
DR IntAct; P18540; 2.
DR PRIDE; P18540; -.
DR EnsemblBacteria; AAK90927; AAK90927; Atu6166.
DR KEGG; atu:Atu6166; -.
DR PATRIC; fig|176299.10.peg.5363; -.
DR HOGENOM; CLU_017728_0_0_5; -.
DR OMA; QFKSHNA; -.
DR BioCyc; AGRO:ATU6166-MON; -.
DR BRENDA; 2.7.13.3; 200.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; ISS:PAMGO_GAT.
DR CDD; cd00082; HisKA; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR045812; DAHL.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR003661; HisK_dim/P.
DR InterPro; IPR036097; HisK_dim/P_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR Pfam; PF19443; DAHL; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF00512; HisKA; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00388; HisKA; 1.
DR SUPFAM; SSF47384; SSF47384; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Crown gall tumor; Kinase; Membrane;
KW Nucleotide-binding; Phosphoprotein; Plasmid; Reference proteome;
KW Transferase; Transmembrane; Transmembrane helix;
KW Two-component regulatory system.
FT CHAIN 1..833
FT /note="Wide host range VirA protein"
FT /id="PRO_0000074895"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..280
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 475..698
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT DOMAIN 720..831
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT MOD_RES 478
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 770
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT CONFLICT 68
FT /note="R -> S (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="L -> V (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 455
FT /note="I -> F (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 551
FT /note="L -> P (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 655..656
FT /note="SC -> CS (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 833 AA; 91322 MW; B514DAF85BDFB2B5 CRC64;
MNGRYSPSRQ DFKTGAKPWS ILALVVAAMI FALMAITSWQ DNETNRAILT QLRAINIDSA
SLQRDVLRAE AGVVANYRPI ISRLGALRKN LENLKRLFKQ SHLVIGNDFS QLLDKLKVSV
DTTDAAVAAF GAQNVLLQDS LASFTRALSI LPKMSSTDQT VENSNELGSL MLRFVRQPSP
ALSLEISHEL DMLQKASGGA EVPIRILARE GRVILSILPR VNDAVNMIQT SDTAEIAERL
ERKCLEAYSL QSVREQRARI FLGSVSVGLC IYIISLVYRL RRKTAWLTRR LDYEEVIKEI
GVCFEGGGAT ASSLNSSAQA ALGIIQRFFN AESCALALVD HGDRWAVESF AAKLPEPVWE
DLALREMVSL ARADERASVF RIMSTRKVSC LPPETPGVSM LLAHKSTDQL IAICSLGYQG
YRLKSCPGEV QLLELATACL CHYIDVRRKQ TECDILERRL EHAERLQAVG TLAGGIAHEF
NNILGAILGY AEMAQNMLRR SSVTRRHIDQ IISSGDRARL IIDQILTLSR KLERVTKPFS
VSELVMEIAP LLRVALQRNI ELKFKFDDKK SVVEGSPLEV QQMLMNLCKN ASQAFTADGQ
IDIIVSRIFV SRQKVLAHGV MPAGDYVLLS VSDDGEGIAE TVLPHIFEPF FTTRSCSGGT
GLGLAAVHGH VSALAGYIDV TSAVGRGTRF DIYLPPSSKK PVSPDAFFGP CKTPRGNGEI
VALIEPDPVL REVYEDKIAA LGYEPVGFKT CADLCNWISK GKQADLVLVD QSSLPENQSA
TALHAAFKTA SIIIGGSDLK MSLSSDDMTS ALFLPKPISS RTMAYAIRTK IKA
