ID   VIRB2_BRUSU             Reviewed;         105 AA.
AC   Q7CEG0; G0KER6; Q7BQL7;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=Type IV secretion system protein virB2;
DE   Flags: Precursor;
GN   Name=virB2; OrderedLocusNames=BRA0068, BS1330_II0068;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA   O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA   Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT   "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT   Ptl type IV secretion systems is essential for intracellular survival of
RT   Brucella suis.";
RL   Mol. Microbiol. 33:1210-1220(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC   STRAIN=1330;
RX   PubMed=11830669; DOI=10.1073/pnas.032514299;
RA   Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA   Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA   O'Callaghan D.;
RT   "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
CC   -!- FUNCTION: The VirB system could be required for the establishment of
CC       the replication niche in the host.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Specifically induced within macrophages by phagosome
CC       acidification. Induced at 37 degrees Celsius in minimal medium,
CC       suggesting that nutritional stress is a regulating signal.
CC   -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC       exponential phase.
CC   -!- SIMILARITY: Belongs to the PtlA family. {ECO:0000305}.
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DR   EMBL; AF141604; AAD56612.1; -; Genomic_DNA.
DR   EMBL; AE014292; AAN33280.1; -; Genomic_DNA.
DR   EMBL; CP002998; AEM19560.1; -; Genomic_DNA.
DR   RefSeq; WP_002967165.1; NZ_KN046805.1.
DR   AlphaFoldDB; Q7CEG0; -.
DR   EnsemblBacteria; AEM19560; AEM19560; BS1330_II0068.
DR   GeneID; 45125487; -.
DR   GeneID; 55591805; -.
DR   KEGG; bms:BRA0068; -.
DR   KEGG; bsi:BS1330_II0068; -.
DR   PATRIC; fig|204722.21.peg.2302; -.
DR   HOGENOM; CLU_155084_1_1_5; -.
DR   OMA; TIMIAWA; -.
DR   PRO; PR:Q7CEG0; -.
DR   Proteomes; UP000007104; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007039; TrbC/VirB2.
DR   Pfam; PF04956; TrbC; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Signal; Transmembrane; Transmembrane helix;
KW   Virulence.
FT   SIGNAL          1..36
FT                   /evidence="ECO:0000255"
FT   CHAIN           37..105
FT                   /note="Type IV secretion system protein virB2"
FT                   /id="PRO_0000291452"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   105 AA;  11107 MW;  837E9B32EA02B120 CRC64;
     MKTASPSKKS LSRILPHLLL ALIVSIAAIE PNLAHANGGL DKVNTSMQKV LDLLSGVSIT
     IVTIAIIWSG YKMAFRHARF MDVVPVLGGA LVVGAAAEIA SYLLR