ID   VIRB3_AGRFC             Reviewed;         108 AA.
AC   P17793;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   27-MAR-2002, sequence version 3.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Protein virB3;
GN   Name=virB3; OrderedLocusNames=Atu6169; ORFNames=AGR_pTi_bx137;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2370849; DOI=10.1007/bf00261729;
RA   Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT   "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT   reading frames.";
RL   Mol. Gen. Genet. 221:256-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA   Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA   Steck T.R., Kado C.I.;
RT   "Molecular characterization of the vir regulon of Agrobacterium
RT   tumefaciens: complete nucleotide sequence and gene organization of the
RT   28.63-kbp regulon cloned as a single unit.";
RL   Plasmid 23:85-106(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA   Shirasu K., Morel P., Kado C.I.;
RT   "Characterization of the virB operon of an Agrobacterium tumefaciens Ti
RT   plasmid: nucleotide sequence and protein analysis.";
RL   Mol. Microbiol. 4:1153-1163(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: VirB proteins are suggested to act at the bacterial surface
CC       and there play an important role in directing T-DNA transfer to plant
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the virB3 family. {ECO:0000305}.
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DR   EMBL; X53264; CAA37356.1; -; Genomic_DNA.
DR   EMBL; J03320; AAA91593.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK90930.1; -; Genomic_DNA.
DR   PIR; AG3248; AG3248.
DR   PIR; S12343; B3AG58.
DR   RefSeq; NP_396489.1; NC_003065.3.
DR   RefSeq; WP_010891501.1; NC_003065.3.
DR   AlphaFoldDB; P17793; -.
DR   EnsemblBacteria; AAK90930; AAK90930; Atu6169.
DR   GeneID; 39477132; -.
DR   KEGG; atu:Atu6169; -.
DR   PATRIC; fig|176299.10.peg.5365; -.
DR   HOGENOM; CLU_158477_1_0_5; -.
DR   OMA; SVDAPFW; -.
DR   PhylomeDB; P17793; -.
DR   BioCyc; AGRO:ATU6169-MON; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR   InterPro; IPR007792; T4SS_VirB3/TrbD/AvhB.
DR   Pfam; PF05101; VirB3; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; Membrane; Plasmid; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..108
FT                   /note="Protein virB3"
FT                   /id="PRO_0000065837"
FT   TRANSMEM        22..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        71
FT                   /note="V -> L (in Ref. 1; CAA37356)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        92..93
FT                   /note="SV -> TL (in Ref. 1; CAA37356)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   108 AA;  11596 MW;  476D3CF9947E73EF CRC64;
     MNDRLEEATL YLAATRPALF LGVPLTLAGL LVMFAGFVIV IVQNPLYEVV LVPLWFGARL
     VVERDYNAAS VVLLFLQTAG RSVDGLIWGG ASVSPNPIKV PARGRGMA