ID   VIRB3_BRUSU             Reviewed;         116 AA.
AC   Q7CEG1; G0KER5; Q7BQL6;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Type IV secretion system protein virB3;
GN   Name=virB3; OrderedLocusNames=BRA0067, BS1330_II0067;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA   O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA   Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT   "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT   Ptl type IV secretion systems is essential for intracellular survival of
RT   Brucella suis.";
RL   Mol. Microbiol. 33:1210-1220(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC   STRAIN=1330;
RX   PubMed=11830669; DOI=10.1073/pnas.032514299;
RA   Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA   Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA   O'Callaghan D.;
RT   "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
CC   -!- FUNCTION: The VirB system could be required for the establishment of
CC       the replication niche in the host.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Specifically induced within macrophages by phagosome
CC       acidification. Induced at 37 degrees Celsius in minimal medium,
CC       suggesting that nutritional stress is a regulating signal.
CC   -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC       exponential phase.
CC   -!- SIMILARITY: Belongs to the virB3 family. {ECO:0000305}.
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DR   EMBL; AF141604; AAD56613.1; -; Genomic_DNA.
DR   EMBL; AE014292; AAN33279.1; -; Genomic_DNA.
DR   EMBL; CP002998; AEM19559.1; -; Genomic_DNA.
DR   RefSeq; WP_002966512.1; NZ_KN046805.1.
DR   AlphaFoldDB; Q7CEG1; -.
DR   EnsemblBacteria; AEM19559; AEM19559; BS1330_II0067.
DR   GeneID; 45125486; -.
DR   GeneID; 55591804; -.
DR   KEGG; bms:BRA0067; -.
DR   KEGG; bsi:BS1330_II0067; -.
DR   PATRIC; fig|204722.21.peg.2303; -.
DR   HOGENOM; CLU_158477_0_0_5; -.
DR   OMA; WIDTKFR; -.
DR   PRO; PR:Q7CEG1; -.
DR   Proteomes; UP000007104; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR007792; T4SS_VirB3/TrbD/AvhB.
DR   Pfam; PF05101; VirB3; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..116
FT                   /note="Type IV secretion system protein virB3"
FT                   /id="PRO_0000290175"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   116 AA;  13082 MW;  1FBAAC743EAFBDD9 CRC64;
     MTTAPQESNA RSAGYRGDPI FKGCTRPAML FGVPVIPLVI VGGSIVLLSV WISMFILPLI
     VPIVLVMRQI TQTDDQMFRL LGLKAQFRLI HFNRTGRFWR ASAYSPIAFT KRKRES