ID   VIRB4_AGRFC             Reviewed;         789 AA.
AC   P17794;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   27-MAR-2002, sequence version 3.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Protein virB4;
DE   Flags: Precursor;
GN   Name=virB4; OrderedLocusNames=Atu6170; ORFNames=AGR_pTi_8;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2370849; DOI=10.1007/bf00261729;
RA   Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT   "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT   reading frames.";
RL   Mol. Gen. Genet. 221:256-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA   Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA   Steck T.R., Kado C.I.;
RT   "Molecular characterization of the vir regulon of Agrobacterium
RT   tumefaciens: complete nucleotide sequence and gene organization of the
RT   28.63-kbp regulon cloned as a single unit.";
RL   Plasmid 23:85-106(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA   Shirasu K., Morel P., Kado C.I.;
RT   "Characterization of the virB operon of an Agrobacterium tumefaciens Ti
RT   plasmid: nucleotide sequence and protein analysis.";
RL   Mol. Microbiol. 4:1153-1163(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: A possible function of virB4 might be to provide the energy,
CC       via hydrolysis of ATP, for translocation of virulence proteins of the
CC       transfer of a T-DNA-protein complex across the agrobacterium membrane.
CC   -!- SIMILARITY: Belongs to the TrbE/VirB4 family. {ECO:0000305}.
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DR   EMBL; X53264; CAA37357.1; -; Genomic_DNA.
DR   EMBL; J03320; AAA91594.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK90932.1; -; Genomic_DNA.
DR   PIR; AH3248; AH3248.
DR   PIR; S12344; B4AG58.
DR   RefSeq; NP_396491.1; NC_003065.3.
DR   RefSeq; WP_010974916.1; NC_003065.3.
DR   AlphaFoldDB; P17794; -.
DR   SMR; P17794; -.
DR   EnsemblBacteria; AAK90932; AAK90932; Atu6170.
DR   GeneID; 39477131; -.
DR   KEGG; atu:Atu6170; -.
DR   PATRIC; fig|176299.10.peg.5366; -.
DR   HOGENOM; CLU_008341_3_0_5; -.
DR   OMA; ITFEKDY; -.
DR   PhylomeDB; P17794; -.
DR   BioCyc; AGRO:ATU6170-MON; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004346; CagE_TrbE_VirB.
DR   InterPro; IPR018145; CagE_TrbE_VirB_cntrl_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF03135; CagE_TrbE_VirB; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00929; VirB4_CagE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Crown gall tumor; Nucleotide-binding; Plasmid;
KW   Reference proteome; Signal.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..789
FT                   /note="Protein virB4"
FT                   /id="PRO_0000022663"
FT   BINDING         433..440
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        55
FT                   /note="A -> R (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        59
FT                   /note="A -> R (in Ref. 1; CAA37357)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        249
FT                   /note="R -> G (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        488..489
FT                   /note="AP -> G (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        527
FT                   /note="E -> Q (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        613
FT                   /note="A -> R (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   789 AA;  87477 MW;  1409A3642445E9CB CRC64;
     MLGASGTTER SGEVYLPYVG HVSDHIVLLE DGSIMTMAHV SGMAFELEDA EMRNARCRAF
     NTLLRNIADD HVSIYAHLVR HDDVPPSPAR HFRSAFSASL SEAFEERVLS GKLLRNDHFL
     TLIVSPRAAL GKVRRRFTKR YRQKENDLTA QTRNLEDLWH LVAGALEAYG LRRLGIREKQ
     DVLFTEVGEA LRLIMTGRFT PVPVVSGSLG ASIYTDRVIC GKRGLEIRTP KDSYVGSIYS
     FREYPATTRP GMLNVLLSLD FPLVLTQSFS FLTRSQAHSK LSLKSSQMLS SGDKAVTQIS
     KLSEAEDALA SNEFVLGAHH VSLCIYANDL NNLADRGARA RTRLADAGAV VVQEGIGMEA
     AYWSQLPGNY KWRTRPGAIT SRNFAGLVSF ENFPEGSGSG HWGNAIARFR TNGGTPFDYI
     PHEHDVGMTA IFGPIGRGKT TLMTFILAML EQSMVDRAGA VVLFDKDRGS ELLVRATGGT
     YLALRRGAPS GLAPLRGLEN TAASHDFLRE WIVALIESDG RGGISPEENR RLVRGIHRQL
     SFDPHMRSIA GLREFLLHGP AEGAGARLQR WCRGNALGWA FDGELDEVKL DPSITGFDMT
     HLLEYEEVCA AAAAYLLHRI GAMVDGRRFV MSCDEFRAYL LNPKFAAVVD KFLLTVRKNN
     GMLILATQQP EHVLESQLGA SLVAQCMTKI FYPSPTADRS AYIDGLKCTE KEFQAIREDM
     AVGSRKFLLK RESGSVVCEF DLREMREYVA VLSGRANTVR FADQLRKVQG DNPSAWLSEF
     MARYHEAKD