ID   VIRB4_BRUA2             Reviewed;         831 AA.
AC   Q2YIT8; Q57A17; Q9KIS9;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Type IV secretion system protein virB4;
GN   Name=virB4; OrderedLocusNames=BAB2_0065;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTION, AND FUNCTION.
RX   PubMed=10940027; DOI=10.1128/jb.182.17.4849-4855.2000;
RA   Sieira R., Comerci D.J., Sanchez D.O., Ugalde R.A.;
RT   "A homologue of an operon required for DNA transfer in Agrobacterium is
RT   required in Brucella abortus for virulence and intracellular
RT   multiplication.";
RL   J. Bacteriol. 182:4849-4855(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
CC   -!- FUNCTION: The virB operon is essential for intracellular survival and
CC       is not involved in the invasion process. Constitutes a major
CC       determinant of virulence in mice. {ECO:0000269|PubMed:10940027}.
CC   -!- MISCELLANEOUS: Transcription is turned on at the beginning of the
CC       stationary phase of vegetative growth.
CC   -!- SIMILARITY: Belongs to the TrbE/VirB4 family. {ECO:0000305}.
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DR   EMBL; AF226278; AAF73897.1; -; Genomic_DNA.
DR   EMBL; AM040265; CAJ12231.1; -; Genomic_DNA.
DR   RefSeq; WP_002966513.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YIT8; -.
DR   SMR; Q2YIT8; -.
DR   STRING; 359391.BAB2_0065; -.
DR   EnsemblBacteria; CAJ12231; CAJ12231; BAB2_0065.
DR   GeneID; 3827979; -.
DR   KEGG; bmf:BAB2_0065; -.
DR   PATRIC; fig|359391.11.peg.2012; -.
DR   HOGENOM; CLU_008341_3_0_5; -.
DR   OMA; HRDLNTL; -.
DR   PhylomeDB; Q2YIT8; -.
DR   BioCyc; MetaCyc:BAB_RS26670-MON; -.
DR   Proteomes; UP000002719; Chromosome II.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004346; CagE_TrbE_VirB.
DR   InterPro; IPR018145; CagE_TrbE_VirB_cntrl_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR043964; P-loop_TraG.
DR   Pfam; PF03135; CagE_TrbE_VirB; 1.
DR   Pfam; PF19044; P-loop_TraG; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00929; VirB4_CagE; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Nucleotide-binding; Reference proteome; Virulence.
FT   CHAIN           1..831
FT                   /note="Type IV secretion system protein virB4"
FT                   /id="PRO_0000290168"
FT   BINDING         457..464
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   831 AA;  94572 MW;  657AAA319B4CC8E5 CRC64;
     MGAQSKYAQQ LNNERSLAPF IPFRSQVGPT TVITRDGDFV RTWRIAGLAF ETQDKEKLLI
     RKDQLNTLFR AIASNNVALW SHNVRRRTWD HLKSFFSNPF CDALDKKYYG SFSGYRMMSN
     ELYLTVIYRP VPAKISRLFN VAVHRSHAEI LQEQQLAIRK LDEIGNQIET SLRRYGGDDG
     RGIEVLSTYE DKHGALCSQQ LEFYNFLLSG EWQKVRVPSC PLDEYLGTGW VYAGTETIEI
     RTANATRYAR GIDFKDYANH TEPGILNGLM YSDYEYVITQ SFSFMTKRDG KEFLTRQKQR
     LQNTEDGSAS QIMEMDIAID QLGRGDFVMG EYHYSLLVFA EDMETVRHNT SHAMNILQDN
     GFLATVIATA TDAAFYAQLP CNWRYRPRVA GLTSLNFAGL SCFHNFRAGK RDGNPWGQAL
     TLLKTPSGQP AYLNFHYSKG DEDNFDKKLL GNTRIIGQSG AGKTVLMNFC LAQAQKYLHN
     APMGMCNVFF DKDQGAKGTI LAIGGKYLAI RNGEPTGFNP FQMEPTAGNI LFLEKLVQVL
     VSRDGQHVTT TDESRISHAI RTVMRMRPEL RRLSTVLQNV TEGSDRQDRE NSVAKRLAKW
     CFDDGTGKRG TFWWVLDCPQ DQIDFNTHSN YGFDGTDFLD NADVRTPISM YLLHRMELAI
     DGRRFIYWMD EAWKWVDDEA FSEFANNKQL TIRKQNGLGV FATQMPSSLL NSKVASALVQ
     QVATEIYLPN PKADYHEYTD GFKVTNEEFD IIRSMSEESR MFLVKQGHHS MICRLELNGF
     DDELAILSGS SDNNELLDQV IAEVGDDPSV WLPVFQERRK ARIASSKSTG R