ID   CAI_PHAVU               Reviewed;          25 AA.
AC   P84708;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Chitinolytic alpha-amylase inhibitor PvCAI;
DE            EC=3.2.1.14;
DE   Flags: Fragment;
OS   Phaseolus vulgaris (Kidney bean) (French bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX   NCBI_TaxID=3885;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, AND MASS
RP   SPECTROMETRY.
RC   STRAIN=G12953 {ECO:0000269|PubMed:16213488};
RC   TISSUE=Cotyledon {ECO:0000269|PubMed:16213488};
RX   PubMed=16213488; DOI=10.1016/j.febslet.2005.09.030;
RA   Dayler C.S.A., Mendes P.A.M., Prates M.V., Bloch C. Jr., Franco O.L.,
RA   Grossi-de-Sa M.F.;
RT   "Identification of a novel bean alpha-amylase inhibitor with chitinolytic
RT   activity.";
RL   FEBS Lett. 579:5616-5620(2005).
CC   -!- FUNCTION: Alpha-amylase inhibitor, active against Z.subfasciatus alpha-
CC       amylase (ZSA) but not porcine pancreatic alpha-amylase (PPA). Has
CC       chitinase activity. {ECO:0000269|PubMed:16213488}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide
CC         (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14;
CC         Evidence={ECO:0000269|PubMed:16213488};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:16213488}.
CC   -!- MASS SPECTROMETRY: Mass=33330; Mass_error=10; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16213488};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 18 family. Chitinase
CC       class II subfamily. {ECO:0000255}.
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DR   AlphaFoldDB; P84708; -.
DR   STRING; 3885.XP_007133284.1; -.
DR   eggNOG; KOG4701; Eukaryota.
DR   GO; GO:0015066; F:alpha-amylase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0004568; F:chitinase activity; IDA:UniProtKB.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Alpha-amylase inhibitor; Carbohydrate metabolism; Chitin degradation;
KW   Direct protein sequencing; Glycosidase; Hydrolase;
KW   Polysaccharide degradation.
FT   CHAIN           1..>25
FT                   /note="Chitinolytic alpha-amylase inhibitor PvCAI"
FT                   /id="PRO_0000077056"
FT   NON_TER         25
FT                   /evidence="ECO:0000303|PubMed:16213488"
SQ   SEQUENCE   25 AA;  2705 MW;  A2BEE14FF9EE2F4C CRC64;
     HEDNAGIAVY WGQDAREGDL VTACN