ID   VIRB6_BRUSU             Reviewed;         347 AA.
AC   Q9RPX9; G0KER2; Q8FXK5;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 2.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Type IV secretion system protein VirB6;
GN   Name=virB6; OrderedLocusNames=BRA0064, BS1330_II0064;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA   O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA   Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT   "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT   Ptl type IV secretion systems is essential for intracellular survival of
RT   Brucella suis.";
RL   Mol. Microbiol. 33:1210-1220(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC   STRAIN=1330;
RX   PubMed=11830669; DOI=10.1073/pnas.032514299;
RA   Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA   Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA   O'Callaghan D.;
RT   "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
CC   -!- FUNCTION: The VirB system could be required for the establishment of
CC       the replication niche in the host.
CC   -!- INTERACTION:
CC       Q9RPX9; Q9RPX5: virB10; NbExp=3; IntAct=EBI-11178687, EBI-7022388;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Specifically induced within macrophages by phagosome
CC       acidification. Induced at 37 degrees Celsius in minimal medium,
CC       suggesting that nutritional stress is a regulating signal.
CC   -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC       exponential phase.
CC   -!- SIMILARITY: Belongs to the TrbL/VirB6 family. {ECO:0000305}.
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DR   EMBL; AF141604; AAD56616.1; -; Genomic_DNA.
DR   EMBL; AE014292; AAN33276.1; -; Genomic_DNA.
DR   EMBL; CP002998; AEM19556.1; -; Genomic_DNA.
DR   RefSeq; WP_002969266.1; NZ_KN046805.1.
DR   AlphaFoldDB; Q9RPX9; -.
DR   IntAct; Q9RPX9; 1.
DR   MINT; Q9RPX9; -.
DR   EnsemblBacteria; AEM19556; AEM19556; BS1330_II0064.
DR   GeneID; 45125483; -.
DR   GeneID; 55591801; -.
DR   KEGG; bms:BRA0064; -.
DR   KEGG; bsi:BS1330_II0064; -.
DR   PATRIC; fig|204722.21.peg.2306; -.
DR   HOGENOM; CLU_065797_0_0_5; -.
DR   OMA; FIVQMFA; -.
DR   PhylomeDB; Q9RPX9; -.
DR   PRO; PR:Q9RPX9; -.
DR   Proteomes; UP000007104; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IEA:InterPro.
DR   InterPro; IPR007688; Conjugal_tfr_TrbL/VirB6.
DR   Pfam; PF04610; TrbL; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..347
FT                   /note="Type IV secretion system protein VirB6"
FT                   /id="PRO_0000290183"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        205..225
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        270..290
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        264..265
FT                   /note="MY -> RN (in Ref. 1; AAD56616)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   347 AA;  36134 MW;  DDAFBAC037970F52 CRC64;
     MVNPVIFEFI GTSIHNQLNN YVTMVASNTM NMIATTAVLA GGLYYTAMGI LMSVGRIEGP
     FSQLVISCIK FMLIAAFALN ISTYSEWVID TVHNMESGFA DAFAGNHGTP SSTIYQTLDN
     SLGKGWNIAA MLFEKGDNRG LTQIVQGFSE LLLSFLVAGS TLILAGPTGA MIVATNAVIA
     ILLGIGPLFI LALGWAPTRG FFDRWFGAIV TSILQVALLS AVLSISSAIF SRMVAAINLA
     SATQSTLFSC LSLTAVTIVM PYMMYKVYEY GGILGSSISA ATISLGSLAV NTATSGGGAM
     TSIFSGSSGG GGSGSAKAGG ESSYSAGGNA MWSPAYRQHV LGQFNRD