VIRB7_AGRFC
ID VIRB7_AGRFC Reviewed; 55 AA.
AC P17797;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Outer membrane lipoprotein virB7;
DE Flags: Precursor;
GN Name=virB7; OrderedLocusNames=Atu6173; ORFNames=AGR_pTi_10.1;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2370849; DOI=10.1007/bf00261729;
RA Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT reading frames.";
RL Mol. Gen. Genet. 221:256-266(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA Shirasu K., Morel P., Kado C.I.;
RT "Characterization of the virB operon of an Agrobacterium tumefaciens Ti
RT plasmid: nucleotide sequence and protein analysis.";
RL Mol. Microbiol. 4:1153-1163(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Is essential for the biogenesis of the T-pilus, which is
CC required for virulence and T-DNA transfer to plant cells. When is
CC associated with virB9, might function as a nucleation center for
CC recruitment of VirB proteins during assembly of the T-DNA transfer
CC machine (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer and heterodimer of virB7 and virB9; disulfide-
CC linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor. Note=Also
CC associated with the T-pilus when is a homodimer, possibly at the pilus
CC base. {ECO:0000250}.
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DR EMBL; X53264; CAA37360.1; -; Genomic_DNA.
DR EMBL; J03320; AAA91597.1; -; Genomic_DNA.
DR EMBL; AE007871; AAL46409.1; -; Genomic_DNA.
DR PIR; AC3249; AC3249.
DR PIR; S12347; B7AG58.
DR RefSeq; NP_536093.1; NC_003065.3.
DR RefSeq; WP_010891497.1; NC_003065.3.
DR AlphaFoldDB; P17797; -.
DR IntAct; P17797; 1.
DR EnsemblBacteria; AAL46409; AAL46409; Atu6173.
DR GeneID; 39477128; -.
DR KEGG; atu:Atu6173; -.
DR HOGENOM; CLU_3021583_0_0_5; -.
DR OMA; PLNVGRW; -.
DR BioCyc; AGRO:ATU6173-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR InterPro; IPR035545; VirB7.
DR Pfam; PF17413; VirB7; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell outer membrane; Crown gall tumor; Disulfide bond; Lipoprotein;
KW Membrane; Palmitate; Plasmid; Reference proteome; Signal; Virulence.
FT SIGNAL 1..14
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 15..55
FT /note="Outer membrane lipoprotein virB7"
FT /id="PRO_0000022669"
FT LIPID 15
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 15
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT DISULFID 24
FT /note="Interchain (with C-262 in virB9); in heterodimeric
FT form"
FT /evidence="ECO:0000250"
FT DISULFID 24
FT /note="Interchain; in homodimeric form"
FT /evidence="ECO:0000250"
FT CONFLICT 13
FT /note="G -> S (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 55 AA; 5872 MW; A323988F153896EF CRC64;
MKYCLLCLAL ALGGCQTNDK LASCKGPIFP LNVGRWQPTP SDLQLSNVGG RHEGV
