CAK1_YEAST
ID CAK1_YEAST Reviewed; 368 AA.
AC P43568; D6VTK1;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Serine/threonine-protein kinase CAK1;
DE EC=2.7.11.22;
DE AltName: Full=CDK-activating kinase;
GN Name=CAK1; Synonyms=CIV1; OrderedLocusNames=YFL029C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8752210; DOI=10.1016/s0092-8674(00)80129-4;
RA Kaldis P., Sutton A., Solomon M.J.;
RT "The Cdk-activating kinase (CAK) from budding yeast.";
RL Cell 86:553-564(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7670463; DOI=10.1038/ng0795-261;
RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA Eki T.;
RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT cerevisiae.";
RL Nat. Genet. 10:261-268(1995).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.22;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.22;
CC -!- INTERACTION:
CC P43568; P00546: CDC28; NbExp=3; IntAct=EBI-3953, EBI-4253;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr
CC protein kinase family. CDC2/CDKX subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U60192; AAB17052.1; -; Genomic_DNA.
DR EMBL; D50617; BAA09209.1; -; Genomic_DNA.
DR EMBL; BK006940; DAA12411.1; -; Genomic_DNA.
DR PIR; S56225; S56225.
DR RefSeq; NP_116624.1; NM_001179937.1.
DR AlphaFoldDB; P43568; -.
DR SMR; P43568; -.
DR BioGRID; 31117; 249.
DR DIP; DIP-1637N; -.
DR IntAct; P43568; 6.
DR MINT; P43568; -.
DR STRING; 4932.YFL029C; -.
DR BindingDB; P43568; -.
DR ChEMBL; CHEMBL5003; -.
DR iPTMnet; P43568; -.
DR MaxQB; P43568; -.
DR PaxDb; P43568; -.
DR PRIDE; P43568; -.
DR EnsemblFungi; YFL029C_mRNA; YFL029C; YFL029C.
DR GeneID; 850515; -.
DR KEGG; sce:YFL029C; -.
DR SGD; S000001865; CAK1.
DR VEuPathDB; FungiDB:YFL029C; -.
DR eggNOG; KOG0594; Eukaryota.
DR GeneTree; ENSGT00940000174760; -.
DR HOGENOM; CLU_000288_181_1_1; -.
DR InParanoid; P43568; -.
DR OMA; SIDITHC; -.
DR BioCyc; YEAST:G3O-30431-MON; -.
DR BRENDA; 2.7.11.22; 984.
DR PRO; PR:P43568; -.
DR Proteomes; UP000002311; Chromosome VI.
DR RNAct; P43568; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0019912; F:cyclin-dependent protein kinase activating kinase activity; IDA:SGD.
DR GO; GO:0004693; F:cyclin-dependent protein serine/threonine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0008353; F:RNA polymerase II CTD heptapeptide repeat kinase activity; IBA:GO_Central.
DR GO; GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.
DR GO; GO:0051321; P:meiotic cell cycle; IMP:SGD.
DR GO; GO:0060633; P:negative regulation of transcription initiation from RNA polymerase II promoter; IGI:SGD.
DR GO; GO:0006468; P:protein phosphorylation; IBA:GO_Central.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome;
KW Serine/threonine-protein kinase; Transferase.
FT CHAIN 1..368
FT /note="Serine/threonine-protein kinase CAK1"
FT /id="PRO_0000085690"
FT DOMAIN 1..368
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT ACT_SITE 156
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
SQ SEQUENCE 368 AA; 42186 MW; 852AFD1E95B90C42 CRC64;
MKLDSIDITH CQLVKSTRTA RIYRSDTYAI KCLALDFDIP PHNAKFEVSI LNKLGNKCKH
ILPLLESKAT DNNDLLLLFP FEEMNLYEFM QMHYKRDRRK KNPYYDLLNP SIPIVADPPV
QKYTNQLDVN RYSLSFFRQM VEGIAFLHEN KIIHRDIKPQ NIMLTNNTST VSPKLYIIDF
GISYDMANNS QTSAEPMDSK VTDISTGIYK APEVLFGVKC YDGGVDVWSL LIIISQWFQR
ETSRMGHVPA MIDDGSDDMN SDGSDFRLIC SIFEKLGIPS IQKWEEVAQH GSVDAFVGMF
GADGDGKYVL DQEKDVQISI VERNMPRLDE IADVKVKQKF INCILGMVSF SPNERWSCQR
ILQELEKP
