ID   VIRB7_RHIRD             Reviewed;          55 AA.
AC   P0A3W4; P09780;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Outer membrane lipoprotein virB7;
DE   Flags: Precursor;
GN   Name=virB7;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3281947; DOI=10.1016/s0021-9258(18)60637-4;
RA   Ward J.E., Akiyoshi D.E., Regier D., Datta A., Gordon M.P., Nester E.W.;
RT   "Characterization of the virB operon from an Agrobacterium tumefaciens Ti
RT   plasmid.";
RL   J. Biol. Chem. 263:5804-5814(1988).
RN   [2]
RP   ERRATUM OF PUBMED:3281947, AND SEQUENCE REVISION.
RX   PubMed=2307685; DOI=10.1016/s0021-9258(19)39628-0;
RA   Ward J.E., Akiyoshi D.E., Regier D., Datta A., Gordon M.P., Nester E.W.;
RL   J. Biol. Chem. 265:4768-4768(1990).
RN   [3]
RP   CHARACTERIZATION, AND MUTAGENESIS OF CYS-15.
RC   STRAIN=A348;
RX   PubMed=8655494; DOI=10.1128/jb.178.11.3156-3167.1996;
RA   Fernandez D., Dang T.A.T., Spudich G.M., Zhou X.-R., Berger B.R.,
RA   Christie P.J.;
RT   "The Agrobacterium tumefaciens virB7 gene product, a proposed component of
RT   the T-complex transport apparatus, is a membrane-associated lipoprotein
RT   exposed at the periplasmic surface.";
RL   J. Bacteriol. 178:3156-3167(1996).
RN   [4]
RP   CHARACTERIZATION, AND MUTAGENESIS OF CYS-15 AND CYS-24.
RC   STRAIN=A348;
RX   PubMed=11371529; DOI=10.1128/jb.183.12.3642-3651.2001;
RA   Sagulenko V., Sagulenko E., Jakubowski S., Spudich E., Christie P.J.;
RT   "VirB7 lipoprotein is exocellular and associates with the Agrobacterium
RT   tumefaciens T pilus.";
RL   J. Bacteriol. 183:3642-3651(2001).
RN   [5]
RP   DISULFIDE BOND, AND MUTAGENESIS OF CYS-15 AND CYS-24.
RX   PubMed=8799123; DOI=10.1073/pnas.93.17.8889;
RA   Anderson L.B., Hertzel A.V., Das A.;
RT   "Agrobacterium tumefaciens VirB7 and VirB9 form a disulfide-linked protein
RT   complex.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:8889-8894(1996).
CC   -!- FUNCTION: Is essential for the biogenesis of the T-pilus, which is
CC       required for virulence and T-DNA transfer to plant cells. When is
CC       associated with virB9, might function as a nucleation center for
CC       recruitment of VirB proteins during assembly of the T-DNA transfer
CC       machine.
CC   -!- SUBUNIT: Homodimer and heterodimer of virB7 and virB9; disulfide-
CC       linked. {ECO:0000269|PubMed:8799123}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane; Lipid-anchor. Note=Also
CC       associated with the T-pilus when is a homodimer, possibly at the pilus
CC       base.
CC   -!- MISCELLANEOUS: Is protease-resistant.
CC   -!- MISCELLANEOUS: Both virB7 lipid modification and disulfide cross-
CC       linking are important for T-pilus assembly.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA88652.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; J03216; AAA88652.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AF242881; AAF77167.1; -; Genomic_DNA.
DR   PIR; H28621; B7AGA6.
DR   RefSeq; NP_059805.1; NC_002377.1.
DR   RefSeq; WP_010892493.1; NZ_QSNU01000012.1.
DR   AlphaFoldDB; P0A3W4; -.
DR   DIP; DIP-335N; -.
DR   IntAct; P0A3W4; 1.
DR   TCDB; 3.A.7.1.1; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR   PATRIC; fig|358.67.peg.5497; -.
DR   OrthoDB; 2069886at2; -.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR035545; VirB7.
DR   Pfam; PF17413; VirB7; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell outer membrane; Crown gall tumor; Disulfide bond; Lipoprotein;
KW   Membrane; Palmitate; Plasmid; Signal; Virulence.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000305"
FT   CHAIN           15..55
FT                   /note="Outer membrane lipoprotein virB7"
FT                   /id="PRO_0000022671"
FT   LIPID           15
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           15
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000305"
FT   DISULFID        24
FT                   /note="Interchain (with C-262 in virB9); in heterodimeric
FT                   form"
FT                   /evidence="ECO:0000269|PubMed:8799123"
FT   DISULFID        24
FT                   /note="Interchain; in homodimeric form"
FT                   /evidence="ECO:0000269|PubMed:8799123"
FT   MUTAGEN         15
FT                   /note="C->S: Loss of activity and destabilization of the
FT                   protein; no T-pilus formation; no loss of virB7-virB9
FT                   complex formation."
FT                   /evidence="ECO:0000269|PubMed:11371529,
FT                   ECO:0000269|PubMed:8655494, ECO:0000269|PubMed:8799123"
FT   MUTAGEN         24
FT                   /note="C->S: No homodimerization, and no virB7-virB9
FT                   complex formation; very low levels of T-pilus production."
FT                   /evidence="ECO:0000269|PubMed:11371529,
FT                   ECO:0000269|PubMed:8799123"
SQ   SEQUENCE   55 AA;  5930 MW;  87909B60286A29B9 CRC64;
     MKYCLLCLVV ALSGCQTNDT IASCKGPIFP LNVGRWQPTP SDLQLRNSGG RYDGA