ID   VIRB8_AGRFC             Reviewed;         237 AA.
AC   P17798;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Protein virB8;
GN   Name=virB8; OrderedLocusNames=Atu6174; ORFNames=AGR_pTi_11;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2370849; DOI=10.1007/bf00261729;
RA   Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT   "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT   reading frames.";
RL   Mol. Gen. Genet. 221:256-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA   Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA   Steck T.R., Kado C.I.;
RT   "Molecular characterization of the vir regulon of Agrobacterium
RT   tumefaciens: complete nucleotide sequence and gene organization of the
RT   28.63-kbp regulon cloned as a single unit.";
RL   Plasmid 23:85-106(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA   Shirasu K., Morel P., Kado C.I.;
RT   "Characterization of the virB operon of an Agrobacterium tumefaciens Ti
RT   plasmid: nucleotide sequence and protein analysis.";
RL   Mol. Microbiol. 4:1153-1163(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 92-237, AND SUBUNIT.
RX   PubMed=16481621; DOI=10.1073/pnas.0511216103;
RA   Bailey S., Ward D., Middleton R., Grossmann J.G., Zambryski P.C.;
RT   "Agrobacterium tumefaciens VirB8 structure reveals potential protein-
RT   protein interaction sites.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:2582-2587(2006).
CC   -!- FUNCTION: VirB proteins are suggested to act at the bacterial surface
CC       and there play an important role in directing T-DNA transfer to plant
CC       cells.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:16481621}.
CC   -!- INTERACTION:
CC       P17798; P17800: virB10; NbExp=5; IntAct=EBI-2012972, EBI-2013080;
CC       P17798; P17798: virB8; NbExp=6; IntAct=EBI-2012972, EBI-2012972;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
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DR   EMBL; X53264; CAA37361.1; -; Genomic_DNA.
DR   EMBL; J03320; AAA91598.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK90936.2; -; Genomic_DNA.
DR   PIR; AD3249; AD3249.
DR   PIR; S12348; B8AG58.
DR   RefSeq; NP_396495.2; NC_003065.3.
DR   RefSeq; WP_010891496.1; NC_003065.3.
DR   PDB; 2CC3; X-ray; 2.20 A; A/B=92-237.
DR   PDBsum; 2CC3; -.
DR   AlphaFoldDB; P17798; -.
DR   SMR; P17798; -.
DR   DIP; DIP-45215N; -.
DR   IntAct; P17798; 2.
DR   MINT; P17798; -.
DR   EnsemblBacteria; AAK90936; AAK90936; Atu6174.
DR   GeneID; 39477127; -.
DR   KEGG; atu:Atu6174; -.
DR   PATRIC; fig|176299.10.peg.5369; -.
DR   HOGENOM; CLU_068461_1_1_5; -.
DR   OMA; VYLWVRP; -.
DR   PhylomeDB; P17798; -.
DR   BioCyc; AGRO:ATU6174-MON; -.
DR   EvolutionaryTrace; P17798; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   Pfam; PF04335; VirB8; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Crown gall tumor;
KW   Membrane; Plasmid; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..237
FT                   /note="Protein virB8"
FT                   /id="PRO_0000065843"
FT   TOPO_DOM        1..38
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..237
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        21..22
FT                   /note="Missing (in Ref. 1; CAA37361)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        128..129
FT                   /note="SA -> TR (in Ref. 2 and 3)"
FT                   /evidence="ECO:0000305"
FT   HELIX           93..110
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   TURN            114..116
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   HELIX           117..126
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   HELIX           130..140
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   HELIX           148..151
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   TURN            152..154
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   STRAND          155..169
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   STRAND          172..183
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   STRAND          189..201
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   HELIX           207..210
FT                   /evidence="ECO:0007829|PDB:2CC3"
FT   STRAND          217..226
FT                   /evidence="ECO:0007829|PDB:2CC3"
SQ   SEQUENCE   237 AA;  26294 MW;  71FEE1084B9597E9 CRC64;
     MKGSEYALLV ARETLAEHYK EVEAFQTARA KSARRLSKVI AAVATIAVLG NVAQAFTIAT
     MVPLIRLVPV YLWIRPDGTV DSEVSVSRLP ATQEEAVVNA SLWEYVRLRE SYDADTAQYA
     YDLVSNFSAP MVRQNYQQFF NYPNPTSPQV ILGKHGRLEV EHIASNDVTP GVQQIRYKRT
     LIVDGKMPMA STWTATVRYE KVTSLPGRLR LTNPGGLVVT SYQTSEDTVS NAGHSEP