ID   VIRB8_AGRT9             Reviewed;         230 AA.
AC   P05357;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Protein virB8;
GN   Name=virB8;
OS   Agrobacterium tumefaciens (strain 15955).
OG   Plasmid pTi15955.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=190386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2837739; DOI=10.1093/nar/16.10.4621;
RA   Thompson D.V., Melchers L.S., Idler K.B., Shilperoort R.A.,
RA   Hooykaas P.J.J.;
RT   "Analysis of the complete nucleotide sequence of the Agrobacterium
RT   tumefaciens virB operon.";
RL   Nucleic Acids Res. 16:4621-4636(1988).
CC   -!- FUNCTION: VirB proteins are suggested to act at the bacterial surface
CC       and there play an important role in directing T-DNA transfer to plant
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAC15165.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X06826; CAC15165.1; ALT_INIT; Genomic_DNA.
DR   PIR; S00784; B8AG55.
DR   AlphaFoldDB; P05357; -.
DR   SMR; P05357; -.
DR   TCDB; 3.A.7.1.1; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR   PRIDE; P05357; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   Pfam; PF04335; VirB8; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Crown gall tumor; Membrane; Plasmid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..230
FT                   /note="Protein virB8"
FT                   /id="PRO_0000065844"
FT   TOPO_DOM        1..38
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..230
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   230 AA;  25408 MW;  92D000C464810D6D CRC64;
     MLVARESLAE HYKEVEAFQT ARAKSARRLS KIIAAVAAIA ILGNVAQAFA IATMVPLSRL
     VPVYLWIRPD GTVDSEVSIS RLPATQEEAV VNASLWEYVR LRESYDADTA QYAYDLVSNF
     SAPTVRQDYQ QFFNYPNPSS PQVILGKRGR VEVEHIASND VTPSTQQIRY KRTLVVDGKM
     PVVSTWTATV RYEKVTSLPG RLRLTNPAGL VVTSYQTSED TVSNVGQGAP