VIRB8_BARHE
ID VIRB8_BARHE Reviewed; 234 AA.
AC Q6G2B4; Q9RND0;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 25-MAY-2022, entry version 79.
DE RecName: Full=Type IV secretion system protein virB8;
GN Name=virB8; OrderedLocusNames=BH13320;
OS Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)
OS (Rochalimaea henselae).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bartonellaceae; Bartonella.
OX NCBI_TaxID=283166;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX PubMed=10882236; DOI=10.1089/10445490050043344;
RA Padmalayam I., Karem K., Baumstark B.R., Massung R.;
RT "The gene encoding the 17-kDa antigen of Bartonella henselae is located
RT within a cluster of genes homologous to the virB virulence operon.";
RL DNA Cell Biol. 19:377-382(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA La Scola B., Holmberg M., Andersson S.G.E.;
RT "The louse-borne human pathogen Bartonella quintana is a genomic derivative
RT of the zoonotic agent Bartonella henselae.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX PubMed=11553594; DOI=10.1128/iai.69.10.6495-6502.2001;
RA Schmiederer M., Arcenas R., Widen R., Valkov N., Anderson B.E.;
RT "Intracellular induction of the Bartonella henselae virB operon by human
RT endothelial cells.";
RL Infect. Immun. 69:6495-6502(2001).
RN [4]
RP INTERACTION WITH VIRB9 AND VIRB10.
RX PubMed=15231811; DOI=10.1128/jb.186.14.4796-4801.2004;
RA Shamaei-Tousi A., Cahill R., Frankel G.;
RT "Interaction between protein subunits of the type IV secretion system of
RT Bartonella henselae.";
RL J. Bacteriol. 186:4796-4801(2004).
RN [5]
RP FUNCTION.
RC STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX PubMed=15049812; DOI=10.1111/j.1365-2958.2003.03964.x;
RA Schmid M.C., Schulein R., Dehio M., Denecker G., Carena I., Dehio C.;
RT "The VirB type IV secretion system of Bartonella henselae mediates
RT invasion, proinflammatory activation and antiapoptotic protection of
RT endothelial cells.";
RL Mol. Microbiol. 52:81-92(2004).
RN [6]
RP FUNCTION.
RC STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX PubMed=15642951; DOI=10.1073/pnas.0406796102;
RA Schulein R., Guye P., Rhomberg T.A., Schmid M.C., Schroeder G.,
RA Vergunst A.C., Carena I., Dehio C.;
RT "A bipartite signal mediates the transfer of type IV secretion substrates
RT of Bartonella henselae into human cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:856-861(2005).
CC -!- FUNCTION: The type IV secretion system VirB/VirD4 is a major virulence
CC determinant for subversion of human endothelial cell (HEC) function.
CC VirB-dependent changes of HEC include massive cytoskeletal
CC rearrangements, a pro-inflammatory activation by nuclear factor NF-
CC kappa-B, inhibition of early and late events of apoptosis, leading to
CC an increased cell survival, and, at high infection doses, a cytostatic
CC or cytotoxic effect, which interfers with a potent VirB-independent
CC mitogenic activity. These changes of HEC require the T4S coupling
CC protein VirD4 and at least one of the effector proteins BepA-G.
CC {ECO:0000269|PubMed:15049812, ECO:0000269|PubMed:15642951}.
CC -!- SUBUNIT: Interacts with virB9 and virB10.
CC {ECO:0000269|PubMed:15231811}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: During the interaction with the intracellular environment of
CC host cells. {ECO:0000269|PubMed:11553594}.
CC -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF00946.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF182718; AAF00946.1; ALT_INIT; Genomic_DNA.
DR EMBL; BX897699; CAF28105.1; -; Genomic_DNA.
DR RefSeq; WP_011181133.1; NZ_LRIJ02000001.1.
DR AlphaFoldDB; Q6G2B4; -.
DR SMR; Q6G2B4; -.
DR STRING; 283166.BH13320; -.
DR PaxDb; Q6G2B4; -.
DR EnsemblBacteria; CAF28105; CAF28105; BH13320.
DR KEGG; bhe:BH13320; -.
DR eggNOG; COG3736; Bacteria.
DR Proteomes; UP000000421; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030255; P:protein secretion by the type IV secretion system; IEA:InterPro.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR007430; VirB8.
DR InterPro; IPR026264; VirB8/PtlE.
DR Pfam; PF04335; VirB8; 1.
DR PIRSF; PIRSF003299; VirB8_PtlE; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW Transmembrane helix; Transport; Virulence.
FT CHAIN 1..234
FT /note="Type IV secretion system protein virB8"
FT /id="PRO_0000273531"
FT TOPO_DOM 1..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..234
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 234 AA; 27135 MW; 0914734C655FAEFC CRC64;
MKHSLRTLWR LRVKINEFNE YIKEARSFDI DRMHGMRQRM RIAMALTVLF GLMTIALALA
VAALTPLKTV EPFVIRVDNS TGIIETVSAL KETPNDYDEA ITRYFASKYV RAREGFQLSE
AEHNFRLVSL LSSPEEQSRF AKWYAGNNPE SPQNIYQNMI ATVTIKSISF LSKDLIQVRY
YKTVRELNDK ENISHWVSIL NFSYINAQIS TQDRLINPLG FQVSEYRSDP EVIQ
