ID   VIRB8_BARQU             Reviewed;         234 AA.
AC   Q6FYW3; Q4L2Q5; Q8KQB8; Q8KQB9;
DT   20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 2.
DT   25-MAY-2022, entry version 83.
DE   RecName: Full=Type IV secretion system protein virB8;
GN   Name=virB8; OrderedLocusNames=BQ10590;
OS   Bartonella quintana (strain Toulouse) (Rochalimaea quintana).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=283165;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Toulouse;
RA   Alsmark U.C.M., Frank A.C., Thollesson M., Andersson S.G.E.;
RT   "Evolution of type IV secretion systems in Bartonella: horizontal
RT   transmission and gene conversion.";
RL   Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC VR-358 / Fuller / CIP 107027;
RA   Kohlhorst D.E., Soni T., Baumstark B.R.;
RT   "Genes composing the virB operon of Bartonella quintana.";
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND POSSIBLE FUNCTION.
RC   STRAIN=Toulouse;
RX   PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA   Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA   Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA   La Scola B., Holmberg M., Andersson S.G.E.;
RT   "The louse-borne human pathogen Bartonella quintana is a genomic derivative
RT   of the zoonotic agent Bartonella henselae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
CC   -!- FUNCTION: Component of the type IV secretion system VirB/VirD4 which
CC       could be a major virulence determinant for subversion of human
CC       endothelial cell (HEC) function.
CC   -!- SUBUNIT: Interacts with virB9 and virB10. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM43802.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAF26526.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AY122055; AAM82241.1; -; Genomic_DNA.
DR   EMBL; AY216720; AAM43802.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AY216720; AAM43803.1; -; Genomic_DNA.
DR   EMBL; BX897700; CAF26526.1; ALT_INIT; Genomic_DNA.
DR   PDB; 4LSO; X-ray; 1.70 A; A=65-234.
DR   PDBsum; 4LSO; -.
DR   AlphaFoldDB; Q6FYW3; -.
DR   SMR; Q6FYW3; -.
DR   STRING; 283165.BQ10590; -.
DR   EnsemblBacteria; CAF26526; CAF26526; BQ10590.
DR   KEGG; bqu:BQ10590; -.
DR   eggNOG; COG3736; Bacteria.
DR   HOGENOM; CLU_068461_1_1_5; -.
DR   OMA; DLIQVRY; -.
DR   Proteomes; UP000000597; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IEA:InterPro.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   InterPro; IPR026264; VirB8/PtlE.
DR   Pfam; PF04335; VirB8; 1.
DR   PIRSF; PIRSF003299; VirB8_PtlE; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport; Virulence.
FT   CHAIN           1..234
FT                   /note="Type IV secretion system protein virB8"
FT                   /id="PRO_0000281412"
FT   TOPO_DOM        1..41
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        42..62
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        63..234
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   HELIX           99..114
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   HELIX           118..120
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   HELIX           121..131
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   HELIX           134..144
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   HELIX           152..155
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   TURN            156..158
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   STRAND          160..172
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   STRAND          175..185
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   STRAND          191..204
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   HELIX           211..216
FT                   /evidence="ECO:0007829|PDB:4LSO"
FT   STRAND          221..230
FT                   /evidence="ECO:0007829|PDB:4LSO"
SQ   SEQUENCE   234 AA;  26929 MW;  ABE9E8B0BA954565 CRC64;
     MKNSLIKIRK SLVKSDAFDE YVKEARSFDI DRMHSLQQRM RIAMTLTVLF GLMTIALALA
     VAALTPLKTV EPFVIRVDNS TGIIETVSAL KETPNNYDEA ITRYFAGKYV RAREGFQLSE
     AEYNFRLISL LSSPEEQNRF AKWYSGNNPE SPQNIYHNMT AKVTIKSISF LSKDLIQVRY
     YKTIRELNGK ENISHWVSIL NFSYINAHIS TEDRLINPLG FQVSEYRSDP EVIK