ID   VIRB8_BRUA2             Reviewed;         239 AA.
AC   Q2YJ78; Q57A21; Q7BMZ6;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Type IV secretion system protein virB8;
GN   Name=virB8; OrderedLocusNames=BAB2_0061;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPTION, AND FUNCTION.
RX   PubMed=10940027; DOI=10.1128/jb.182.17.4849-4855.2000;
RA   Sieira R., Comerci D.J., Sanchez D.O., Ugalde R.A.;
RT   "A homologue of an operon required for DNA transfer in Agrobacterium is
RT   required in Brucella abortus for virulence and intracellular
RT   multiplication.";
RL   J. Bacteriol. 182:4849-4855(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
CC   -!- FUNCTION: The virB operon is essential for intracellular survival and
CC       is not involved in the invasion process. Constitutes a major
CC       determinant of virulence in mice. {ECO:0000269|PubMed:10940027}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Transcription is turned on at the beginning of the
CC       stationary phase of vegetative growth.
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
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DR   EMBL; AF226278; AAF73901.1; -; Genomic_DNA.
DR   EMBL; AM040265; CAJ12227.1; -; Genomic_DNA.
DR   RefSeq; WP_002966517.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YJ78; -.
DR   SMR; Q2YJ78; -.
DR   IntAct; Q2YJ78; 2.
DR   STRING; 359391.BAB2_0061; -.
DR   EnsemblBacteria; CAJ12227; CAJ12227; BAB2_0061.
DR   GeneID; 45053159; -.
DR   GeneID; 55591799; -.
DR   KEGG; bmf:BAB2_0061; -.
DR   PATRIC; fig|359391.11.peg.2009; -.
DR   HOGENOM; CLU_068461_1_1_5; -.
DR   OMA; DLIQVRY; -.
DR   PhylomeDB; Q2YJ78; -.
DR   BioCyc; MetaCyc:BAB_RS26650-MON; -.
DR   PRO; PR:Q2YJ78; -.
DR   Proteomes; UP000002719; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IEA:InterPro.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   InterPro; IPR026264; VirB8/PtlE.
DR   Pfam; PF04335; VirB8; 1.
DR   PIRSF; PIRSF003299; VirB8_PtlE; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Virulence.
FT   CHAIN           1..239
FT                   /note="Type IV secretion system protein virB8"
FT                   /id="PRO_0000291389"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   239 AA;  26446 MW;  68F44CBCF7018023 CRC64;
     MFGRKQSPQK SVKNGQGNAP SVYDEALNWE AAHVRLVEKS ERRAWKIAGA FGTITVLLGI
     GIAGMLPLKQ HVPYLVRVNA QTGAPDILTS LDEKSVSYDT VMDKYWLSQY VIARETYDWY
     TLQKDYETVG MLSSPSEGQS YASQFQGDKA LDKQYGSNVR TSVTIVSIVP NGKGIGTVRF
     AKTTKRTNET GDGETTHWIA TIGYQYVNPS LMSESARLTN PLGFNVTSYR VDPEMGVVQ