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VIRB8_BRUSU
ID   VIRB8_BRUSU             Reviewed;         239 AA.
AC   Q7CEG3; G0KER0; Q7BQL4;
DT   12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Type IV secretion system protein virB8;
GN   Name=virB8; OrderedLocusNames=BRA0062, BS1330_II0062;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA   O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA   Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT   "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT   Ptl type IV secretion systems is essential for intracellular survival of
RT   Brucella suis.";
RL   Mol. Microbiol. 33:1210-1220(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC   STRAIN=1330;
RX   PubMed=11830669; DOI=10.1073/pnas.032514299;
RA   Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA   Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA   O'Callaghan D.;
RT   "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 77-239.
RX   PubMed=15764702; DOI=10.1073/pnas.0408927102;
RA   Terradot L., Bayliss R., Oomen C., Leonard G.A., Baron C., Waksman G.;
RT   "Structures of two core subunits of the bacterial type IV secretion system,
RT   VirB8 from Brucella suis and ComB10 from Helicobacter pylori.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:4596-4601(2005).
CC   -!- FUNCTION: The VirB system could be required for the establishment of
CC       the replication niche in the host.
CC   -!- INTERACTION:
CC       Q7CEG3; Q9RPX5: virB10; NbExp=5; IntAct=EBI-6407073, EBI-7022388;
CC       Q7CEG3; Q7CEG3: virB8; NbExp=6; IntAct=EBI-6407073, EBI-6407073;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: Specifically induced within macrophages by phagosome
CC       acidification. Induced at 37 degrees Celsius in minimal medium,
CC       suggesting that nutritional stress is a regulating signal.
CC   -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC       exponential phase.
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
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DR   EMBL; AF141604; AAD56618.1; -; Genomic_DNA.
DR   EMBL; AE014292; AAN33274.1; -; Genomic_DNA.
DR   EMBL; CP002998; AEM19554.1; -; Genomic_DNA.
DR   RefSeq; WP_002966517.1; NZ_KN046805.1.
DR   PDB; 2BHM; X-ray; 2.40 A; A/B/C/D/E=77-239.
DR   PDB; 4AKY; X-ray; 2.60 A; A/B/C/D/E=97-234.
DR   PDB; 4AKZ; X-ray; 2.25 A; A/B/C/D/E=97-234.
DR   PDB; 5JBS; X-ray; 1.95 A; A/B/C/D=97-238.
DR   PDBsum; 2BHM; -.
DR   PDBsum; 4AKY; -.
DR   PDBsum; 4AKZ; -.
DR   PDBsum; 5JBS; -.
DR   AlphaFoldDB; Q7CEG3; -.
DR   SMR; Q7CEG3; -.
DR   DIP; DIP-61163N; -.
DR   IntAct; Q7CEG3; 2.
DR   MINT; Q7CEG3; -.
DR   EnsemblBacteria; AEM19554; AEM19554; BS1330_II0062.
DR   GeneID; 45053159; -.
DR   GeneID; 55591799; -.
DR   KEGG; bms:BRA0062; -.
DR   KEGG; bsi:BS1330_II0062; -.
DR   PATRIC; fig|204722.21.peg.2308; -.
DR   HOGENOM; CLU_068461_1_1_5; -.
DR   OMA; DLIQVRY; -.
DR   PhylomeDB; Q7CEG3; -.
DR   EvolutionaryTrace; Q7CEG3; -.
DR   PRO; PR:Q7CEG3; -.
DR   Proteomes; UP000007104; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IEA:InterPro.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   InterPro; IPR026264; VirB8/PtlE.
DR   Pfam; PF04335; VirB8; 1.
DR   PIRSF; PIRSF003299; VirB8_PtlE; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..239
FT                   /note="Type IV secretion system protein virB8"
FT                   /id="PRO_0000291392"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   HELIX           98..115
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   TURN            119..121
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   HELIX           122..132
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   HELIX           135..142
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   HELIX           143..145
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   STRAND          146..149
FT                   /evidence="ECO:0007829|PDB:2BHM"
FT   HELIX           151..155
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   STRAND          158..170
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   STRAND          175..186
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   STRAND          188..190
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   STRAND          194..206
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   HELIX           209..211
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   HELIX           214..219
FT                   /evidence="ECO:0007829|PDB:5JBS"
FT   STRAND          224..233
FT                   /evidence="ECO:0007829|PDB:5JBS"
SQ   SEQUENCE   239 AA;  26446 MW;  68F44CBCF7018023 CRC64;
     MFGRKQSPQK SVKNGQGNAP SVYDEALNWE AAHVRLVEKS ERRAWKIAGA FGTITVLLGI
     GIAGMLPLKQ HVPYLVRVNA QTGAPDILTS LDEKSVSYDT VMDKYWLSQY VIARETYDWY
     TLQKDYETVG MLSSPSEGQS YASQFQGDKA LDKQYGSNVR TSVTIVSIVP NGKGIGTVRF
     AKTTKRTNET GDGETTHWIA TIGYQYVNPS LMSESARLTN PLGFNVTSYR VDPEMGVVQ
 
 
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