ID   VIRB8_RHIRD             Reviewed;         230 AA.
AC   P09781;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1991, sequence version 2.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Protein virB8;
GN   Name=virB8;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3281947; DOI=10.1016/s0021-9258(18)60637-4;
RA   Ward J.E., Akiyoshi D.E., Regier D., Datta A., Gordon M.P., Nester E.W.;
RT   "Characterization of the virB operon from an Agrobacterium tumefaciens Ti
RT   plasmid.";
RL   J. Biol. Chem. 263:5804-5814(1988).
RN   [2]
RP   ERRATUM OF PUBMED:3281947, AND SEQUENCE REVISION.
RX   PubMed=2307685; DOI=10.1016/s0021-9258(19)39628-0;
RA   Ward J.E., Akiyoshi D.E., Regier D., Datta A., Gordon M.P., Nester E.W.;
RL   J. Biol. Chem. 265:4768-4768(1990).
CC   -!- FUNCTION: VirB proteins are suggested to act at the bacterial surface
CC       and there play an important role in directing T-DNA transfer to plant
CC       cells.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the virB8 family. {ECO:0000305}.
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DR   EMBL; J03216; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; I28621; B8AGA6.
DR   AlphaFoldDB; P09781; -.
DR   SMR; P09781; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR032710; NTF2-like_dom_sf.
DR   InterPro; IPR007430; VirB8.
DR   Pfam; PF04335; VirB8; 1.
DR   SUPFAM; SSF54427; SSF54427; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Crown gall tumor; Membrane; Plasmid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..230
FT                   /note="Protein virB8"
FT                   /id="PRO_0000065845"
FT   TOPO_DOM        1..38
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        39..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        62..230
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   230 AA;  25382 MW;  87CB1A7A8B30BD76 CRC64;
     MLVARESLAE HYKEVEAFQT ARAKSARRLS KIIAAVAAIA ILGNVAQAFA IATMVPLSRL
     VPVYLWIRAD GTVDSEVSIS RLPATQEEAV VNASLWEYVR LRESYDADTA QYAYDLVSNF
     SAPTVRQDYQ QFFNYPNPSS PQVILGKRGR VEVEHIASND VTPSTQQIRY KRTLVVDGKM
     PVVSTWTATV RYEKVTSLPG RLRLTNPAGL VVTSYQTSED TVSNVGQGAP