VIRB9_AGRFC
ID VIRB9_AGRFC Reviewed; 293 AA.
AC P17799;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein virB9;
DE Flags: Precursor;
GN Name=virB9; OrderedLocusNames=Atu6175; ORFNames=AGR_pTi_12;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2370849; DOI=10.1007/bf00261729;
RA Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT reading frames.";
RL Mol. Gen. Genet. 221:256-266(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA Shirasu K., Morel P., Kado C.I.;
RT "Characterization of the virB operon of an Agrobacterium tumefaciens Ti
RT plasmid: nucleotide sequence and protein analysis.";
RL Mol. Microbiol. 4:1153-1163(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Is essential for the biogenesis of the T-pilus, which is
CC required for virulence and T-DNA transfer to plant cells. When is
CC associated with virB7, might function as a nucleation center for
CC recruitment of VirB proteins during assembly of the T-DNA transfer
CC machine (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of virB7 and virB9; disulfide-linked.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC P17799; P17800: virB10; NbExp=3; IntAct=EBI-2012881, EBI-2013080;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TrbG/VirB9 family. {ECO:0000305}.
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DR EMBL; X53264; CAA37362.1; -; Genomic_DNA.
DR EMBL; J03320; AAA91599.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90937.1; -; Genomic_DNA.
DR PIR; AE3249; AE3249.
DR PIR; S12349; B9AG58.
DR RefSeq; NP_396496.1; NC_003065.3.
DR RefSeq; WP_010891495.1; NC_003065.3.
DR AlphaFoldDB; P17799; -.
DR SMR; P17799; -.
DR IntAct; P17799; 3.
DR EnsemblBacteria; AAK90937; AAK90937; Atu6175.
DR GeneID; 39477126; -.
DR KEGG; atu:Atu6175; -.
DR HOGENOM; CLU_058585_3_0_5; -.
DR OMA; FGPRNWA; -.
DR PhylomeDB; P17799; -.
DR BioCyc; AGRO:ATU6175-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR CDD; cd06911; VirB9_CagX_TrbG; 1.
DR Gene3D; 2.60.40.2500; -; 1.
DR InterPro; IPR010258; Conjugal_tfr_TrbG/VirB9/CagX.
DR InterPro; IPR014148; VirB9.
DR InterPro; IPR033645; VirB9/CagX/TrbG_C.
DR InterPro; IPR038161; VirB9/CagX/TrbG_C_sf.
DR Pfam; PF03524; CagX; 1.
DR TIGRFAMs; TIGR02781; VirB9; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Crown gall tumor; Disulfide bond; Membrane; Plasmid;
KW Reference proteome; Signal; Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..293
FT /note="Protein virB9"
FT /id="PRO_0000022672"
FT DISULFID 262
FT /note="Interchain (with C-24 in virB7)"
FT /evidence="ECO:0000250"
FT CONFLICT 146
FT /note="R -> P (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 293 AA; 32201 MW; 5BBB7A7D36A6689F CRC64;
MTKKAFLTLA CLLFAAIGAR AEDTPTAGRL DPRMRYLAYN PDQVVRLSTA VGATLVVTFG
ANETVTAVAV SNSKDLAALP RGNYLFFKAS KVLPPQPVVV LTASDAGMRR YVFSISSKTL
PHLDKEQADL YYSVQFAYPA DDAAARQKAA QEKAVADRIR AEAQYQQRAE GLLEQPATTV
GAEDKNWHYV AQGDRSLLPL EVFDDGFTTV FHFPGNVRIP SIYTINPDGK EAVANYSVKG
SYVEISSVSR GWRLRDGHTV LCIWNTAYDP VGRRPETGTV RPDVKRVLKE VRG
