ID   VIRB9_AGRT9             Reviewed;         293 AA.
AC   P0A3W7; P05358; P09782;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Protein virB9;
DE   Flags: Precursor;
GN   Name=virB9;
OS   Agrobacterium tumefaciens (strain 15955).
OG   Plasmid pTi15955.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=190386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2837739; DOI=10.1093/nar/16.10.4621;
RA   Thompson D.V., Melchers L.S., Idler K.B., Shilperoort R.A.,
RA   Hooykaas P.J.J.;
RT   "Analysis of the complete nucleotide sequence of the Agrobacterium
RT   tumefaciens virB operon.";
RL   Nucleic Acids Res. 16:4621-4636(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Winans S.C., Zhu J., Oger P.M., Schrammeijer B., Hooykaas P.J.,
RA   Farrand S.K.;
RT   "Octopine-type Ti plasmid sequence.";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Is essential for the biogenesis of the T-pilus, which is
CC       required for virulence and T-DNA transfer to plant cells. When is
CC       associated with virB7, might function as a nucleation center for
CC       recruitment of VirB proteins during assembly of the T-DNA transfer
CC       machine (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer of virB7 and virB9; disulfide-linked.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral membrane
CC       protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TrbG/VirB9 family. {ECO:0000305}.
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DR   EMBL; X06826; CAA29979.1; -; Genomic_DNA.
DR   PIR; S00785; B9AG55.
DR   RefSeq; NP_059807.1; NC_002377.1.
DR   AlphaFoldDB; P0A3W7; -.
DR   SMR; P0A3W7; -.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd06911; VirB9_CagX_TrbG; 1.
DR   Gene3D; 2.60.40.2500; -; 1.
DR   InterPro; IPR010258; Conjugal_tfr_TrbG/VirB9/CagX.
DR   InterPro; IPR014148; VirB9.
DR   InterPro; IPR033645; VirB9/CagX/TrbG_C.
DR   InterPro; IPR038161; VirB9/CagX/TrbG_C_sf.
DR   Pfam; PF03524; CagX; 1.
DR   TIGRFAMs; TIGR02781; VirB9; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Crown gall tumor; Disulfide bond; Membrane; Plasmid; Signal;
KW   Virulence.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..293
FT                   /note="Protein virB9"
FT                   /id="PRO_0000022673"
FT   DISULFID        262
FT                   /note="Interchain (with C-24 in virB7)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   293 AA;  32172 MW;  1BA2E48058E7DD19 CRC64;
     MTRKALFILA CLFAAATGAE AEDTPMAGKL DPRMRYLAYN PDQVVRLSTA VGATLVVTFA
     TNETVTSVAV SNSKDLAALP RGNYLFFKAS QVLTPQPVIV LTASDSGMRR YVFSISSKTL
     SHLDKEQPDL YYSVQFAYPA DDAAARRREA QQRAVVDRLH AEAQYQRKAE DLLDQPVTAL
     GATDSNWHYV AQGDRSLLPL EVFDNGFTTV FHFPGNVRIP SIYTINPDGK EAVANYSVKG
     SDVEISSVSR GWRLRDGHTV LCIWNAAYDP VGQRPQTGTV RPDVKRVLKG AKG