VIRB9_RHIRD
ID VIRB9_RHIRD Reviewed; 293 AA.
AC P0A3W6; P05358; P09782;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 70.
DE RecName: Full=Protein virB9;
DE Flags: Precursor;
GN Name=virB9;
OS Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS radiobacter).
OG Plasmid pTiA6.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=358;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3281947; DOI=10.1016/s0021-9258(18)60637-4;
RA Ward J.E., Akiyoshi D.E., Regier D., Datta A., Gordon M.P., Nester E.W.;
RT "Characterization of the virB operon from an Agrobacterium tumefaciens Ti
RT plasmid.";
RL J. Biol. Chem. 263:5804-5814(1988).
RN [2]
RP ERRATUM OF PUBMED:3281947, AND SEQUENCE REVISION.
RX PubMed=2307685; DOI=10.1016/s0021-9258(19)39628-0;
RA Ward J.E., Akiyoshi D.E., Regier D., Datta A., Gordon M.P., Nester E.W.;
RL J. Biol. Chem. 265:4768-4768(1990).
RN [3]
RP DISULFIDE BOND, AND MUTAGENESIS OF CYS-262.
RX PubMed=8799123; DOI=10.1073/pnas.93.17.8889;
RA Anderson L.B., Hertzel A.V., Das A.;
RT "Agrobacterium tumefaciens VirB7 and VirB9 form a disulfide-linked protein
RT complex.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:8889-8894(1996).
CC -!- FUNCTION: Is essential for the biogenesis of the T-pilus, which is
CC required for virulence and T-DNA transfer to plant cells. When is
CC associated with virB7, might function as a nucleation center for
CC recruitment of VirB proteins during assembly of the T-DNA transfer
CC machine.
CC -!- SUBUNIT: Heterodimer of virB7 and virB9; disulfide-linked.
CC {ECO:0000269|PubMed:8799123}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the TrbG/VirB9 family. {ECO:0000305}.
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DR EMBL; J03216; AAA88654.1; -; Genomic_DNA.
DR EMBL; AF242881; AAF77169.1; -; Genomic_DNA.
DR PIR; S00785; B9AG55.
DR RefSeq; NP_059807.1; NC_002377.1.
DR RefSeq; WP_010892495.1; NZ_QSNU01000012.1.
DR AlphaFoldDB; P0A3W6; -.
DR SMR; P0A3W6; -.
DR IntAct; P0A3W6; 1.
DR TCDB; 3.A.7.1.1; the type iv (conjugal dna-protein transfer or virb) secretory pathway (ivsp) family.
DR OrthoDB; 1251301at2; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR CDD; cd06911; VirB9_CagX_TrbG; 1.
DR Gene3D; 2.60.40.2500; -; 1.
DR InterPro; IPR010258; Conjugal_tfr_TrbG/VirB9/CagX.
DR InterPro; IPR014148; VirB9.
DR InterPro; IPR033645; VirB9/CagX/TrbG_C.
DR InterPro; IPR038161; VirB9/CagX/TrbG_C_sf.
DR Pfam; PF03524; CagX; 1.
DR TIGRFAMs; TIGR02781; VirB9; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Crown gall tumor; Disulfide bond; Membrane; Plasmid; Signal;
KW Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..293
FT /note="Protein virB9"
FT /id="PRO_0000022674"
FT DISULFID 262
FT /note="Interchain (with C-24 in virB7)"
FT /evidence="ECO:0000269|PubMed:8799123"
FT MUTAGEN 262
FT /note="C->S: No virB7-virB9 complex formation."
FT /evidence="ECO:0000269|PubMed:8799123"
SQ SEQUENCE 293 AA; 32172 MW; 1BA2E48058E7DD19 CRC64;
MTRKALFILA CLFAAATGAE AEDTPMAGKL DPRMRYLAYN PDQVVRLSTA VGATLVVTFA
TNETVTSVAV SNSKDLAALP RGNYLFFKAS QVLTPQPVIV LTASDSGMRR YVFSISSKTL
SHLDKEQPDL YYSVQFAYPA DDAAARRREA QQRAVVDRLH AEAQYQRKAE DLLDQPVTAL
GATDSNWHYV AQGDRSLLPL EVFDNGFTTV FHFPGNVRIP SIYTINPDGK EAVANYSVKG
SDVEISSVSR GWRLRDGHTV LCIWNAAYDP VGQRPQTGTV RPDVKRVLKG AKG