VIRBA_AGRFC
ID VIRBA_AGRFC Reviewed; 377 AA.
AC P17800;
DT 01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 2.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Protein virB10;
GN Name=virB10; OrderedLocusNames=Atu6176; ORFNames=AGR_pTi_13;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2370849; DOI=10.1007/bf00261729;
RA Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT reading frames.";
RL Mol. Gen. Genet. 221:256-266(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA Shirasu K., Morel P., Kado C.I.;
RT "Characterization of the virB operon of an Agrobacterium tumefaciens Ti
RT plasmid: nucleotide sequence and protein analysis.";
RL Mol. Microbiol. 4:1153-1163(1990).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: VirB proteins are suggested to act at the bacterial surface
CC and there play an important role in directing T-DNA transfer to plant
CC cells.
CC -!- INTERACTION:
CC P17800; P17800: virB10; NbExp=3; IntAct=EBI-2013080, EBI-2013080;
CC P17800; P17798: virB8; NbExp=5; IntAct=EBI-2013080, EBI-2012972;
CC P17800; P17799: virB9; NbExp=3; IntAct=EBI-2013080, EBI-2012881;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TrbI/VirB10 family. {ECO:0000305}.
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DR EMBL; X53264; CAA37363.1; -; Genomic_DNA.
DR EMBL; J03320; AAA91600.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90938.1; -; Genomic_DNA.
DR PIR; AF3249; AF3249.
DR PIR; S12350; B0AG58.
DR RefSeq; NP_396497.1; NC_003065.3.
DR RefSeq; WP_010891494.1; NC_003065.3.
DR AlphaFoldDB; P17800; -.
DR SMR; P17800; -.
DR IntAct; P17800; 2.
DR MINT; P17800; -.
DR EnsemblBacteria; AAK90938; AAK90938; Atu6176.
DR GeneID; 39477125; -.
DR KEGG; atu:Atu6176; -.
DR PATRIC; fig|176299.10.peg.5370; -.
DR HOGENOM; CLU_041899_1_0_5; -.
DR OMA; TVDNHFW; -.
DR PhylomeDB; P17800; -.
DR BioCyc; AGRO:ATU6176-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR CDD; cd16429; VirB10; 1.
DR Gene3D; 2.40.128.260; -; 1.
DR InterPro; IPR005498; T4SS_VirB10/TraB/TrbI.
DR InterPro; IPR042217; T4SS_VirB10/TrbI.
DR Pfam; PF03743; TrbI; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Crown gall tumor; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..377
FT /note="Protein virB10"
FT /id="PRO_0000065846"
FT TRANSMEM 33..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 53..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..145
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 297
FT /note="A -> P (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 40555 MW; F91BB4CFCC3FE29D CRC64;
MNDDNQQSAH DVDASGSLVS DTHHRRLSGA QKLIVGGVVL ALSLSLIWLG GREKKENGDA
PPSTMIATNT KPFHPAPIDV TLDPPAAQEA VQPTAPPPAR SEPERHEPRP EETPIFAYTS
GDQGTSKRVQ QGETDRRREG NGEDSPLPKV EVSAENDLSI RMKPTELQPT RATLLPHPDF
MVTEGTIIPC ILQTAIDTSL AGYVKCVLPW DVRGTTNNVV LLDRGTTVVG EIQRGLQQGD
ARVFVLWDRA ETPDHAMISL ASPSADELGR SGLPGTVDNH FWQRFSGAML LSVVQGAFQA
ASTYAGSSGG GTSFNSVQNN GEQTADTALK ATINIPPTLK KNQGDTVSIF VARDLDFSGI
YQLRMAGRAA RGRDRRP
