ID   VIRBA_BARHE             Reviewed;         396 AA.
AC   Q6G2B2; Q9RNC8;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Type IV secretion system protein virB10;
GN   Name=virB10; OrderedLocusNames=BH13340;
OS   Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)
OS   (Rochalimaea henselae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=283166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=10882236; DOI=10.1089/10445490050043344;
RA   Padmalayam I., Karem K., Baumstark B.R., Massung R.;
RT   "The gene encoding the 17-kDa antigen of Bartonella henselae is located
RT   within a cluster of genes homologous to the virB virulence operon.";
RL   DNA Cell Biol. 19:377-382(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA   Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA   Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA   La Scola B., Holmberg M., Andersson S.G.E.;
RT   "The louse-borne human pathogen Bartonella quintana is a genomic derivative
RT   of the zoonotic agent Bartonella henselae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
RN   [3]
RP   INDUCTION.
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=11553594; DOI=10.1128/iai.69.10.6495-6502.2001;
RA   Schmiederer M., Arcenas R., Widen R., Valkov N., Anderson B.E.;
RT   "Intracellular induction of the Bartonella henselae virB operon by human
RT   endothelial cells.";
RL   Infect. Immun. 69:6495-6502(2001).
RN   [4]
RP   INTERACTION WITH VIRB4; VIRB8 AND VIRB9.
RX   PubMed=15231811; DOI=10.1128/jb.186.14.4796-4801.2004;
RA   Shamaei-Tousi A., Cahill R., Frankel G.;
RT   "Interaction between protein subunits of the type IV secretion system of
RT   Bartonella henselae.";
RL   J. Bacteriol. 186:4796-4801(2004).
RN   [5]
RP   FUNCTION.
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15049812; DOI=10.1111/j.1365-2958.2003.03964.x;
RA   Schmid M.C., Schulein R., Dehio M., Denecker G., Carena I., Dehio C.;
RT   "The VirB type IV secretion system of Bartonella henselae mediates
RT   invasion, proinflammatory activation and antiapoptotic protection of
RT   endothelial cells.";
RL   Mol. Microbiol. 52:81-92(2004).
RN   [6]
RP   FUNCTION.
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15642951; DOI=10.1073/pnas.0406796102;
RA   Schulein R., Guye P., Rhomberg T.A., Schmid M.C., Schroeder G.,
RA   Vergunst A.C., Carena I., Dehio C.;
RT   "A bipartite signal mediates the transfer of type IV secretion substrates
RT   of Bartonella henselae into human cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:856-861(2005).
CC   -!- FUNCTION: The type IV secretion system VirB/VirD4 is a major virulence
CC       determinant for subversion of human endothelial cell (HEC) function.
CC       VirB-dependent changes of HEC include massive cytoskeletal
CC       rearrangements, a pro-inflammatory activation by nuclear factor NF-
CC       kappa-B, inhibition of early and late events of apoptosis, leading to
CC       an increased cell survival, and, at high infection doses, a cytostatic
CC       or cytotoxic effect, which interfers with a potent VirB-independent
CC       mitogenic activity. These changes of HEC require the T4S coupling
CC       protein VirD4 and at least one of the effector proteins BepA-G.
CC       {ECO:0000269|PubMed:15049812, ECO:0000269|PubMed:15642951}.
CC   -!- SUBUNIT: Interacts with virB4, virB8 and virB9.
CC       {ECO:0000269|PubMed:15231811}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- INDUCTION: During the interaction with the intracellular environment of
CC       host cells. {ECO:0000269|PubMed:11553594}.
CC   -!- SIMILARITY: Belongs to the TrbI/VirB10 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF00948.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AF182718; AAF00948.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; BX897699; CAF28107.1; -; Genomic_DNA.
DR   RefSeq; WP_011181135.1; NC_005956.1.
DR   RefSeq; WP_034448091.1; NZ_LRIJ02000001.1.
DR   AlphaFoldDB; Q6G2B2; -.
DR   SMR; Q6G2B2; -.
DR   IntAct; Q6G2B2; 2.
DR   STRING; 283166.BH13340; -.
DR   PaxDb; Q6G2B2; -.
DR   EnsemblBacteria; CAF28107; CAF28107; BH13340.
DR   KEGG; bhe:BH13340; -.
DR   eggNOG; COG2948; Bacteria.
DR   OMA; NFIVAMG; -.
DR   Proteomes; UP000000421; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   CDD; cd16429; VirB10; 1.
DR   Gene3D; 2.40.128.260; -; 2.
DR   InterPro; IPR005498; T4SS_VirB10/TraB/TrbI.
DR   InterPro; IPR042217; T4SS_VirB10/TrbI.
DR   Pfam; PF03743; TrbI; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport; Virulence.
FT   CHAIN           1..396
FT                   /note="Type IV secretion system protein virB10"
FT                   /id="PRO_0000273532"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          126..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        8..26
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        126..140
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   396 AA;  43654 MW;  C15EB347F45061DC CRC64;
     MNDPMDENNL LNDRDMIKDG HGKKQRPNTS KAAALVILFG VCLYLAYSTL FTEKQQPVEV
     QKEGIIKQTE LFRPAPPKPV SLEPTIEKNN VLLPKVELPT PPKKTTNSDD SLLEAAQRAP
     VLAYANTQKG QGSTEKNKDI SANQPEAKPD ETAQRFNHLL KPTTLEGIRA AKLGNRNYII
     AMGASIPCIL ETAISSDQQG FASCIVSRDI LSDNGRVVLL DKGTQIVGEY RAGLKKGQKR
     LFVLWNRAKT PNGIIITLAS PATDALGRSG MDGDIDNHWL ERIGSALLVS IVKDATNYVK
     GRLPKDQDKN NSETISSGQN IANIAVENYA NIPPTLSKNQ GEMVNVFVAR DLDFSNVYKL
     KVIENKKQIV NRALSRNFYK NSAVICNEPK LAHIER