CAL1_ORYSJ
ID CAL1_ORYSJ Reviewed; 80 AA.
AC Q6K209;
DT 23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Defensin-like protein CAL1 {ECO:0000305};
DE AltName: Full=OsCPT1 {ECO:0000303|PubMed:19279197};
DE AltName: Full=Pathogen-related protein 12 {ECO:0000305};
DE Short=OsPR12 {ECO:0000305};
DE AltName: Full=Protein CADMIUM ACCUMULATION IN LEAF 1 {ECO:0000303|PubMed:29440679};
DE Flags: Precursor;
GN Name=CAL1 {ECO:0000303|PubMed:29440679};
GN Synonyms=CPT1 {ECO:0000303|PubMed:19279197}, PR12 {ECO:0000305};
GN OrderedLocusNames=Os02g0629800 {ECO:0000312|EMBL:BAF09407.1},
GN LOC_Os02g41904 {ECO:0000305};
GN ORFNames=B1469H02.30 {ECO:0000312|EMBL:BAD23741.1},
GN OsJ_07607 {ECO:0000312|EMBL:EEE57415.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [5]
RP INDUCTION.
RX PubMed=19279197; DOI=10.1104/pp.108.133454;
RA Ma Q., Dai X., Xu Y., Guo J., Liu Y., Chen N., Xiao J., Zhang D., Xu Z.,
RA Zhang X., Chong K.;
RT "Enhanced tolerance to chilling stress in OsMYB3R-2 transgenic rice is
RT mediated by alteration in cell cycle and ectopic expression of stress
RT genes.";
RL Plant Physiol. 150:244-256(2009).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY CADMIUM,
RP BIOTECHNOLOGY, AND MUTAGENESIS OF CYS-34; CYS-45; CYS-51; CYS-55; CYS-65;
RP LEU-70; CYS-74; CYS-76 AND CYS-80.
RX PubMed=29440679; DOI=10.1038/s41467-018-03088-0;
RA Luo J.S., Huang J., Zeng D.L., Peng J.S., Zhang G.B., Ma H.L., Guan Y.,
RA Yi H.Y., Fu Y.L., Han B., Lin H.X., Qian Q., Gong J.M.;
RT "A defensin-like protein drives cadmium efflux and allocation in rice.";
RL Nat. Commun. 9:645-645(2018).
CC -!- FUNCTION: Plant defensin-like protein involved in accumulation of
CC cadmium (Cd) in rice leaves. Mediates Cd efflux from cytosol into
CC extracellular spaces via chelation. This drives Cd secretion from xylem
CC parenchyma cells into the xylem vessels, hence lowering Cd levels in
CC cytosol meanwhile promoting Cd translocation from roots to shoots.
CC {ECO:0000269|PubMed:29440679}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space
CC {ECO:0000269|PubMed:29440679}.
CC -!- TISSUE SPECIFICITY: Expressed preferentially in root exodermis and
CC xylem parenchyma cells in vasculature of root and flag leaf sheath.
CC {ECO:0000269|PubMed:29440679}.
CC -!- INDUCTION: Induced by cadmium in roots (PubMed:29440679). Down-
CC regulated by cold stress (PubMed:19279197).
CC {ECO:0000269|PubMed:19279197, ECO:0000269|PubMed:29440679}.
CC -!- BIOTECHNOLOGY: Rice varieties with high CAL1 activity over-accumulate
CC cadmium (Cd) in rice straws, but are not affected by Cd accumulation,
CC or the accumulation of other essential metals in rice grains. These
CC functional characteristics could be used to breed dual-function rice
CC varieties that produce safe grains while remediating paddy soils.
CC {ECO:0000269|PubMed:29440679}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR EMBL; AP006168; BAD23741.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF09407.1; -; Genomic_DNA.
DR EMBL; AP014958; BAS79891.1; -; Genomic_DNA.
DR EMBL; CM000139; EEE57415.1; -; Genomic_DNA.
DR RefSeq; XP_015624063.1; XM_015768577.1.
DR PDB; 6LCQ; X-ray; 1.62 A; A/B=32-80.
DR PDBsum; 6LCQ; -.
DR AlphaFoldDB; Q6K209; -.
DR SMR; Q6K209; -.
DR STRING; 4530.OS02T0629800-01; -.
DR PaxDb; Q6K209; -.
DR PRIDE; Q6K209; -.
DR EnsemblPlants; Os02t0629800-01; Os02t0629800-01; Os02g0629800.
DR GeneID; 4330051; -.
DR Gramene; Os02t0629800-01; Os02t0629800-01; Os02g0629800.
DR KEGG; osa:4330051; -.
DR eggNOG; ENOG502STQ5; Eukaryota.
DR HOGENOM; CLU_161668_1_1_1; -.
DR InParanoid; Q6K209; -.
DR OMA; EGATRKC; -.
DR OrthoDB; 1625543at2759; -.
DR PlantReactome; R-OSA-6787011; Jasmonic acid signaling.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000007752; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0046870; F:cadmium ion binding; IDA:UniProtKB.
DR GO; GO:0055073; P:cadmium ion homeostasis; IMP:UniProtKB.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cadmium; Disulfide bond; Metal-binding; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..80
FT /note="Defensin-like protein CAL1"
FT /id="PRO_5013533355"
FT DISULFID 34..80
FT /evidence="ECO:0000250|UniProtKB:P69241"
FT DISULFID 45..65
FT /evidence="ECO:0000250|UniProtKB:P69241"
FT DISULFID 51..74
FT /evidence="ECO:0000250|UniProtKB:P69241"
FT DISULFID 55..76
FT /evidence="ECO:0000250|UniProtKB:P69241"
FT MUTAGEN 34
FT /note="C->A: No effect on the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 45
FT /note="C->A: No effect on the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 51
FT /note="C->A: No effect on the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 55
FT /note="C->A: Decreases the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 65
FT /note="C->A: Decreases the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 70
FT /note="L->V: Decreases the ability to bind cadmium; No
FT effect on the localization to the extracellular space."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 74
FT /note="C->A: Decreases the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 76
FT /note="C->A: No effect on the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT MUTAGEN 80
FT /note="C->A: No effect on the ability to bind cadmium."
FT /evidence="ECO:0000269|PubMed:29440679"
FT STRAND 33..37
FT /evidence="ECO:0007829|PDB:6LCQ"
FT HELIX 48..57
FT /evidence="ECO:0007829|PDB:6LCQ"
FT STRAND 61..68
FT /evidence="ECO:0007829|PDB:6LCQ"
FT STRAND 71..79
FT /evidence="ECO:0007829|PDB:6LCQ"
SQ SEQUENCE 80 AA; 8784 MW; 3326C0461F10A1CA CRC64;
MAPSRRMVAS AFLLLAILVA TEMGTTKVAE ARHCLSQSHR FKGMCVSSNN CANVCRTESF
PDGECKSHGL ERKCFCKKVC
