VIRBA_BRUSU
ID VIRBA_BRUSU Reviewed; 391 AA.
AC Q9RPX5; G0KEQ8; Q8FXK6;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 2.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Type IV secretion system protein virB10;
GN Name=virB10; OrderedLocusNames=BRA0060, BS1330_II0060;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT Ptl type IV secretion systems is essential for intracellular survival of
RT Brucella suis.";
RL Mol. Microbiol. 33:1210-1220(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC STRAIN=1330;
RX PubMed=11830669; DOI=10.1073/pnas.032514299;
RA Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA O'Callaghan D.;
RT "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between animal
RT and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/jb.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
CC -!- FUNCTION: The VirB system could be required for the establishment of
CC the replication niche in the host.
CC -!- INTERACTION:
CC Q9RPX5; Q9RPX9: virB6; NbExp=3; IntAct=EBI-7022388, EBI-11178687;
CC Q9RPX5; Q7CEG3: virB8; NbExp=5; IntAct=EBI-7022388, EBI-6407073;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Specifically induced within macrophages by phagosome
CC acidification. Induced at 37 degrees Celsius in minimal medium,
CC suggesting that nutritional stress is a regulating signal.
CC -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC exponential phase.
CC -!- SIMILARITY: Belongs to the TrbI/VirB10 family. {ECO:0000305}.
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DR EMBL; AF141604; AAD56620.1; -; Genomic_DNA.
DR EMBL; AE014292; AAN33272.1; -; Genomic_DNA.
DR EMBL; CP002998; AEM19552.1; -; Genomic_DNA.
DR RefSeq; WP_004689962.1; NZ_KN046805.1.
DR AlphaFoldDB; Q9RPX5; -.
DR SMR; Q9RPX5; -.
DR DIP; DIP-61161N; -.
DR IntAct; Q9RPX5; 2.
DR MINT; Q9RPX5; -.
DR PRIDE; Q9RPX5; -.
DR EnsemblBacteria; AEM19552; AEM19552; BS1330_II0060.
DR GeneID; 45053157; -.
DR GeneID; 55591797; -.
DR KEGG; bms:BRA0060; -.
DR KEGG; bsi:BS1330_II0060; -.
DR PATRIC; fig|204722.22.peg.2852; -.
DR HOGENOM; CLU_041899_6_0_5; -.
DR OMA; PGKDRNT; -.
DR PhylomeDB; Q9RPX5; -.
DR PRO; PR:Q9RPX5; -.
DR Proteomes; UP000007104; Chromosome II.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR CDD; cd16429; VirB10; 1.
DR Gene3D; 2.40.128.260; -; 2.
DR InterPro; IPR005498; T4SS_VirB10/TraB/TrbI.
DR InterPro; IPR042217; T4SS_VirB10/TrbI.
DR Pfam; PF03743; TrbI; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Transmembrane; Transmembrane helix; Virulence.
FT CHAIN 1..391
FT /note="Type IV secretion system protein virB10"
FT /id="PRO_0000291388"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 76..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 78..105
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 83
FT /note="P -> A (in Ref. 1; AAD56620)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="D -> E (in Ref. 1; AAD56620)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="R -> H (in Ref. 1; AAD56620)"
FT /evidence="ECO:0000305"
FT CONFLICT 389
FT /note="K -> Q (in Ref. 1; AAD56620)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 391 AA; 41484 MW; A7720A28B30194B6 CRC64;
MTQENIPVQP GTLDGERGLP TVNENGSGRT RKVLLFLFVV GFIVVLLLLL VFHMRGNAEN
NHHSDKTMVQ TSTVPMRTFK LPPPPPPPPP APPEPPAPPP APAMPIAEPA AAALSLPPLP
DDTPAKDDVL DKSASALMVV TKSSGDTNAQ TAGDTVVQTT NARIQALLDS QKNTKQDAGS
LGTLLHGTQT DARMASLLRN RDFLLAKGSI INCALQTRLD STVPGMAACV VTRNMYSDNG
KVLLIERGST ISGEYDANVK QGMARIYVLW TRVKTPNGVV IDLDSPGADP LGGAGLPGYI
DSHFWKRFGG ALMLSTIETL GRYATQKVGG GGSNQINLNT GGGESTSNLA STALKDTINI
PPTLYKNQGE EIGIYIARDL DFSSVYDVKP K
