ID   CALA_BRAOB              Reviewed;         150 AA.
AC   Q6R4R9; Q39376; Q8GTF3;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Truncated transcription factor CAULIFLOWER A;
DE            Short=BobCAL-a;
DE   AltName: Full=Agamous-like MADS-box protein CAL-A;
GN   Name=CAL-A; Synonyms=CAL1;
OS   Brassica oleracea var. botrytis (Cauliflower).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3715;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower meristem;
RX   PubMed=7824951; DOI=10.1126/science.7824951;
RA   Kempin S.A., Savidge B., Yanofsky M.F.;
RT   "Molecular basis of the cauliflower phenotype in Arabidopsis.";
RL   Science 267:522-525(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. CA25; TISSUE=Flower meristem;
RA   Kop E.P.;
RT   "MADS-box genes and the genetics of cauliflower curd development.";
RL   Thesis (2002), University of Warwick, United Kingdom.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Flower meristem;
RA   He Y.-K., Cao W.-G., Shen R.-J., Liu P.-L.;
RL   Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   TISSUE SPECIFICITY.
RC   TISSUE=Flower;
RX   PubMed=9084216; DOI=10.1007/bf01007702;
RA   Carr S.M., Irish V.F.;
RT   "Floral homeotic gene expression defines developmental arrest stages in
RT   Brassica oleracea L. vars. botrytis and italica.";
RL   Planta 201:179-188(1997).
RN   [5]
RP   FUNCTION.
RC   STRAIN=cv. H6;
RX   PubMed=10765981; DOI=10.1038/sj.cr.7290033;
RA   Li X.F., Shen R.J., Liu P.L., Tang Z.C., He Y.K.;
RT   "Molecular characters and morphological genetics of CAL gene in Chinese
RT   cabbage.";
RL   Cell Res. 10:29-38(2000).
RN   [6]
RP   REVIEW, AND GENE FAMILY.
RX   PubMed=10835404; DOI=10.1093/genetics/155.2.855;
RA   Purugganan M.D., Boyles A.L., Suddith J.I.;
RT   "Variation and selection at the CAULIFLOWER floral homeotic gene
RT   accompanying the evolution of domesticated Brassica oleracea.";
RL   Genetics 155:855-862(2000).
CC   -!- FUNCTION: Probable transcription factor that promotes early floral
CC       meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. Is
CC       required subsequently for the transition of an inflorescence meristem
CC       into a floral meristem. Seems to be partially redundant to the function
CC       of APETALA1. {ECO:0000269|PubMed:10765981}.
CC   -!- SUBUNIT: Homodimer capable of binding to CArG-box sequences.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00251}.
CC   -!- TISSUE SPECIFICITY: Expressed in some of the meristems of arrest-stage
CC       cauliflower heads. {ECO:0000269|PubMed:9084216}.
CC   -!- MISCELLANEOUS: This is a truncated version of CAULIFLOWER that may
CC       contribute to the 'cauliflower'-shaped floral meristem (curd). This
CC       trait has likely been selected by early farmers during the
CC       domestication of modified inflorescence structures.
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DR   EMBL; L36927; AAA64791.1; -; mRNA.
DR   EMBL; AJ505847; CAD47855.1; -; mRNA.
DR   EMBL; AY514052; AAS67310.1; -; mRNA.
DR   PIR; T14457; T14457.
DR   AlphaFoldDB; Q6R4R9; -.
DR   SMR; Q6R4R9; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IEA:InterPro.
DR   CDD; cd00265; MADS_MEF2_like; 1.
DR   Gene3D; 3.40.1810.10; -; 1.
DR   InterPro; IPR033896; MADS_MEF2-like.
DR   InterPro; IPR002487; TF_Kbox.
DR   InterPro; IPR002100; TF_MADSbox.
DR   InterPro; IPR036879; TF_MADSbox_sf.
DR   Pfam; PF01486; K-box; 1.
DR   Pfam; PF00319; SRF-TF; 1.
DR   PRINTS; PR00404; MADSDOMAIN.
DR   SMART; SM00432; MADS; 1.
DR   SUPFAM; SSF55455; SSF55455; 1.
DR   PROSITE; PS51297; K_BOX; 1.
DR   PROSITE; PS00350; MADS_BOX_1; 1.
DR   PROSITE; PS50066; MADS_BOX_2; 1.
PE   2: Evidence at transcript level;
KW   Activator; Coiled coil; Developmental protein; Differentiation;
KW   DNA-binding; Flowering; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..150
FT                   /note="Truncated transcription factor CAULIFLOWER A"
FT                   /id="PRO_0000417140"
FT   DOMAIN          1..61
FT                   /note="MADS-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00251"
FT   DOMAIN          90..150
FT                   /note="K-box; partial"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00629"
FT   CONFLICT        135
FT                   /note="D -> G (in Ref. 3; AAS67310)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   150 AA;  17841 MW;  F48E6512113DBFD9 CRC64;
     MGRGRVEMKR IENKINRQVT FSKRRAGLLK KAHEISILCD AEVSLIVFSH KGKLFEYSSE
     SCMEKVLERY ERYSYAEKQL KAPDSHVNAQ TNWSMEYSRL KAKIELWERN QRHYLGEDLE
     SISIKELQNL EQQLDTSLKH IRSRKNQLMH