VIRBB_BRUSU
ID VIRBB_BRUSU Reviewed; 361 AA.
AC Q8FXK7; G0KEQ7; Q9RPX4;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Type IV secretion system protein VirB11;
GN Name=virB11; OrderedLocusNames=BRA0059, BS1330_II0059;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=10510235; DOI=10.1046/j.1365-2958.1999.01569.x;
RA O'Callaghan D., Cazevieille C., Allardet-Servent A., Boschiroli M.L.,
RA Bourg G., Foulongne V., Frutos P., Kulakov Y., Ramuz M.;
RT "A homologue of the Agrobacterium tumefaciens VirB and Bordetella pertussis
RT Ptl type IV secretion systems is essential for intracellular survival of
RT Brucella suis.";
RL Mol. Microbiol. 33:1210-1220(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND EXPRESSION CONDITIONS.
RC STRAIN=1330;
RX PubMed=11830669; DOI=10.1073/pnas.032514299;
RA Boschiroli M.L., Ouahrani-Bettache S., Foulongne V., Michaux-Charachon S.,
RA Bourg G., Allardet-Servent A., Cazevieille C., Liautard J.P., Ramuz M.,
RA O'Callaghan D.;
RT "The Brucella suis virB operon is induced intracellularly in macrophages.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:1544-1549(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between animal
RT and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/jb.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
CC -!- FUNCTION: The VirB system could be required for the establishment of
CC the replication niche in the host.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Specifically induced within macrophages by phagosome
CC acidification. Induced at 37 degrees Celsius in minimal medium,
CC suggesting that nutritional stress is a regulating signal.
CC -!- MISCELLANEOUS: Transcription of the operon is maximal in early
CC exponential phase.
CC -!- SIMILARITY: Belongs to the GSP E family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD56621.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF141604; AAD56621.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AE014292; AAN33271.1; -; Genomic_DNA.
DR EMBL; CP002998; AEM19551.1; -; Genomic_DNA.
DR PDB; 2GZA; X-ray; 2.60 A; A/B/C=1-361.
DR PDBsum; 2GZA; -.
DR AlphaFoldDB; Q8FXK7; -.
DR SMR; Q8FXK7; -.
DR EnsemblBacteria; AEM19551; AEM19551; BS1330_II0059.
DR KEGG; bms:BRA0059; -.
DR KEGG; bsi:BS1330_II0059; -.
DR HOGENOM; CLU_005379_3_1_5; -.
DR OMA; EAWVEKK; -.
DR EvolutionaryTrace; Q8FXK7; -.
DR PRO; PR:Q8FXK7; -.
DR Proteomes; UP000007104; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043684; C:type IV secretion system complex; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0044097; P:secretion by the type IV secretion system; IEA:InterPro.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR001482; T2SS/T4SS.
DR InterPro; IPR014155; VirB11.
DR Pfam; PF00437; T2SSE; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR02788; VirB11; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Cytoplasm; Nucleotide-binding; Virulence.
FT CHAIN 1..361
FT /note="Type IV secretion system protein VirB11"
FT /id="PRO_0000291439"
FT BINDING 182..189
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 125
FT /note="T -> P (in Ref. 1; AAD56621)"
FT /evidence="ECO:0000305"
FT CONFLICT 207
FT /note="T -> A (in Ref. 1; AAD56621)"
FT /evidence="ECO:0000305"
FT HELIX 13..19
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 21..25
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 30..33
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 38..45
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 48..53
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 56..61
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 67..80
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 86..89
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 91..95
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:2GZA"
FT TURN 107..109
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 116..120
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 129..134
FT /evidence="ECO:0007829|PDB:2GZA"
FT TURN 135..138
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 150..161
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 164..173
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 178..186
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 188..196
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 205..212
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 220..226
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 241..248
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 254..259
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 265..274
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 281..285
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 289..300
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 306..308
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 311..321
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 324..329
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 333..335
FT /evidence="ECO:0007829|PDB:2GZA"
FT STRAND 338..345
FT /evidence="ECO:0007829|PDB:2GZA"
FT HELIX 347..352
FT /evidence="ECO:0007829|PDB:2GZA"
SQ SEQUENCE 361 AA; 40727 MW; D9DDD62F91D2CD3D CRC64;
MMSNRSDFIV PDEAAVKRAA SVNFHLEPLR PWLDDPQITE VCVNRPGEVF CERASAWEYY
AVPNLDYEHL ISLGTATARF VDQDISDSRP VLSAILPMGE RIQIVRPPAC EHGTISVTIR
KPSFTRRTLE DYAQQGFFKH VRPMSKSLTP FEQELLALKE AGDYMSFLRR AVQLERVIVV
AGETGSGKTT LMKALMQEIP FDQRLITIED VPELFLPDHP NHVHLFYPSE AKEEENAPVT
AATLLRSCLR MKPTRILLAE LRGGEAYDFI NVAASGHGGS ITSCHAGSCE LTFERLALMV
LQNRQGRQLP YEIIRRLLYL VVDVVVHVHN GVHDGTGRHI SEVWYDPNTK RALSLQHSEK
T
