VIRB_SHIFL
ID VIRB_SHIFL Reviewed; 309 AA.
AC P0A247; P11656;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Virulence regulon transcriptional activator VirB;
DE AltName: Full=Cell invasion regulator InvE;
GN Name=virB; Synonyms=invE; OrderedLocusNames=CP0123;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, Plasmid pINV_F6_M1382, and
OG Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6200 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=2474742; DOI=10.1111/j.1365-2958.1989.tb00210.x;
RA Adler B., Sasakawa C., Tobe T., Makino S., Komatsu K., Yoshikawa M.;
RT "A dual transcriptional activation system for the 230 kb plasmid genes
RT coding for virulence-associated antigens of Shigella flexneri.";
RL Mol. Microbiol. 3:627-635(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M1382 / Serotype 6; PLASMID=pINV_F6_M1382;
RX PubMed=14573649; DOI=10.1128/iai.71.11.6298-6306.2003;
RA Lan R., Stevenson G., Reeves P.R.;
RT "Comparison of two major forms of the Shigella virulence plasmid pINV:
RT positive selection is a major force driving the divergence.";
RL Infect. Immun. 71:6298-6306(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
CC -!- FUNCTION: Transcription activator for the invasion antigens IpaB, IpaC
CC and IpaD. VirB is itself regulated by VirF.
CC -!- SIMILARITY: Belongs to the ParB family. {ECO:0000305}.
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DR EMBL; X14340; CAA32525.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05798.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18441.1; -; Genomic_DNA.
DR EMBL; AY206439; AAP78986.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72295.1; -; Genomic_DNA.
DR PIR; S04379; S04379.
DR RefSeq; NP_085285.1; NC_002698.1.
DR RefSeq; NP_858256.1; NC_004851.1.
DR RefSeq; WP_000227976.1; NZ_WPGS01000043.1.
DR RefSeq; YP_009062480.1; NC_024996.1.
DR PDB; 3VWB; X-ray; 2.42 A; A=129-250.
DR PDB; 3W2A; X-ray; 2.78 A; A=129-250.
DR PDB; 3W3C; X-ray; 2.43 A; A=129-250.
DR PDBsum; 3VWB; -.
DR PDBsum; 3W2A; -.
DR PDBsum; 3W3C; -.
DR AlphaFoldDB; P0A247; -.
DR SMR; P0A247; -.
DR STRING; 198214.CP0123; -.
DR PRIDE; P0A247; -.
DR EnsemblBacteria; AAL72295; AAL72295; SF_p0123.
DR GeneID; 1237991; -.
DR KEGG; sfl:CP0123; -.
DR PATRIC; fig|198214.7.peg.5378; -.
DR HOGENOM; CLU_065144_0_0_6; -.
DR OMA; CIDANEG; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR InterPro; IPR004437; ParB/RepB/Spo0J.
DR InterPro; IPR003115; ParB/Sulfiredoxin_dom.
DR InterPro; IPR036086; ParB/Sulfiredoxin_sf.
DR InterPro; IPR014884; ParB_fam_C.
DR Pfam; PF08775; ParB; 1.
DR Pfam; PF02195; ParBc; 1.
DR SMART; SM00470; ParB; 1.
DR SUPFAM; SSF110849; SSF110849; 1.
DR TIGRFAMs; TIGR00180; parB_part; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; DNA-binding; Plasmid; Reference proteome;
KW Transcription; Transcription regulation; Virulence.
FT CHAIN 1..309
FT /note="Virulence regulon transcriptional activator VirB"
FT /id="PRO_0000178702"
FT DNA_BIND 152..171
FT /note="H-T-H motif"
FT /evidence="ECO:0000250"
FT HELIX 134..140
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 142..145
FT /evidence="ECO:0007829|PDB:3VWB"
FT TURN 146..148
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 151..158
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 162..172
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 176..180
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 185..187
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 190..205
FT /evidence="ECO:0007829|PDB:3VWB"
FT TURN 211..213
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 214..222
FT /evidence="ECO:0007829|PDB:3VWB"
FT STRAND 227..229
FT /evidence="ECO:0007829|PDB:3VWB"
FT HELIX 234..245
FT /evidence="ECO:0007829|PDB:3VWB"
SQ SEQUENCE 309 AA; 35408 MW; AD5FEEBEBFF6C0C4 CRC64;
MVDLCNDLLS IKEGQKKEFT LHSGNKVSFI KAKIPHKRIQ DLTFVNQKTN VRDQESLTEE
SLADIIKTIK LQQFFPVIGR EIDGRIEILD GTRRRASAIY AGADLEVLYS KEYISTLDAR
KLANDIQTAK EHSIRELGIG LNFLKVSGMS YKDIAKKENL SRAKVTRAFQ AASVPQEIIS
LFPIASELNF NDYKILFNYY KGLEKANESL SSTLPILKEE IKDLDTNLPP DIYKKEILNI
IKKSKNRKQN PSLKVDSLFI SKDKRTYIKR KENKTNRTLI FTLSKINKTV QREIDEAIRD
IISRHLSSS
