ID   CALA_CALS5              Reviewed;          80 AA.
AC   P60976;
DT   26-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2004, sequence version 1.
DT   25-MAY-2022, entry version 48.
DE   RecName: Full=U1-nemetoxin-Csp1a;
DE            Short=U1-NETX-Csp1a;
DE   AltName: Full=Toxic peptide A {ECO:0000303|Ref.1};
DE   Flags: Precursor;
OS   Calisoga sp. (Spider).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Nemesiidae; Calisoga; unclassified Calisoga.
OX   NCBI_TaxID=269418;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 42-80, TOXIC DOSE, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RA   Johnson J.H., Kral R.M. Jr., Krapcho K.;
RT   "Insecticidal peptides from spider venom.";
RL   Patent number US5688764, 18-NOV-1997.
CC   -!- FUNCTION: Causes paralysis to insect larvae (H.virescens). This toxin
CC       is active only on insects.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin. {ECO:0000250}.
CC   -!- MASS SPECTROMETRY: Mass=4304.01; Method=Electrospray;
CC       Evidence={ECO:0000269|Ref.1};
CC   -!- TOXIC DOSE: PD(50) is 2.37 +/- 0.12 mg/kg by injection into the abdomen
CC       of larvae of H.virescens. {ECO:0000269|Ref.1}.
CC   -!- SIMILARITY: Belongs to the neurotoxin 13 (insecticidal toxin ABC)
CC       family. 02 (Calisoga) subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P60976; -.
DR   SMR; P60976; -.
DR   ArachnoServer; AS000028; U1-nemetoxin-Csp1a.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR012626; Spider_insecticidal_peptide.
DR   Pfam; PF08091; Toxin_21; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Knottin; Secreted; Signal; Toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..41
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000035592"
FT   CHAIN           42..80
FT                   /note="U1-nemetoxin-Csp1a"
FT                   /id="PRO_0000035593"
FT   DISULFID        42..56
FT                   /evidence="ECO:0000250|UniProtKB:P49268"
FT   DISULFID        49..60
FT                   /evidence="ECO:0000250|UniProtKB:P49268"
FT   DISULFID        55..77
FT                   /evidence="ECO:0000250|UniProtKB:P49268"
FT   DISULFID        66..73
FT                   /evidence="ECO:0000250|UniProtKB:P49268"
SQ   SEQUENCE   80 AA;  8946 MW;  255AD0C14A137A8E CRC64;
     MKYFVVFCVL IIAVAAFTSA AEDGEVFEEN PLEFPKTIQK RCISARYPCS NSKDCCSGNC
     GTFWTCYIRK DPCSKECLAP