ID   VIRD1_AGRFC             Reviewed;         147 AA.
AC   P18591; P06520;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=T-DNA border endonuclease VirD1;
DE            EC=3.1.-.-;
GN   Name=virD1; OrderedLocusNames=Atu6181; ORFNames=AGR_pTi_19;
OS   Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS   (strain C58)).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2986128; DOI=10.1073/pnas.82.9.2669;
RA   Hagiya M., Close T.J., Tait R.C., Kado C.I.;
RT   "Identification of pTiC58 plasmid-encoded proteins for virulence in
RT   Agrobacterium tumefaciens.";
RL   Proc. Natl. Acad. Sci. U.S.A. 82:2669-2673(1985).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA   Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA   Steck T.R., Kado C.I.;
RT   "Molecular characterization of the vir regulon of Agrobacterium
RT   tumefaciens: complete nucleotide sequence and gene organization of the
RT   28.63-kbp regulon cloned as a single unit.";
RL   Plasmid 23:85-106(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2165478; DOI=10.1128/jb.172.8.4432-4440.1990;
RA   Wang K., Herrera-Estrella A., Van Montagu M.;
RT   "Overexpression of virD1 and virD2 genes in Agrobacterium tumefaciens
RT   enhances T-complex formation and plant transformation.";
RL   J. Bacteriol. 172:4432-4440(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA   Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA   Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA   Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA   Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA   Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA   Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA   Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA   Gordon M.P., Olson M.V., Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA   Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA   Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA   Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA   Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: Tumor formation by A.tumefaciens involves the transfer and
CC       integration of a defined segment (T-DNA) of Ti plasmid DNA into the
CC       plant nuclear genome. The virD operon encodes a site-specific
CC       endonuclease that cleaves at a unique site within both 24 bp direct
CC       repeats flanking the T-DNA.
CC   -!- INTERACTION:
CC       P18591; P07165: virC1; NbExp=4; IntAct=EBI-7412667, EBI-7412683;
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DR   EMBL; M11311; AAA98365.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; J03320; AAA91603.1; -; Genomic_DNA.
DR   EMBL; M33673; AAA22110.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK90943.2; -; Genomic_DNA.
DR   PIR; A37763; A37763.
DR   PIR; AC3250; AC3250.
DR   RefSeq; NP_396502.2; NC_003065.3.
DR   RefSeq; WP_010891489.1; NC_003065.3.
DR   AlphaFoldDB; P18591; -.
DR   IntAct; P18591; 1.
DR   MINT; P18591; -.
DR   EnsemblBacteria; AAK90943; AAK90943; Atu6181.
DR   GeneID; 39477120; -.
DR   KEGG; atu:Atu6181; -.
DR   PATRIC; fig|176299.10.peg.5375; -.
DR   HOGENOM; CLU_1754963_0_0_5; -.
DR   OMA; SAEYESF; -.
DR   BioCyc; AGRO:ATU6181-MON; -.
DR   Proteomes; UP000000813; Plasmid Ti.
DR   GO; GO:0003677; F:DNA binding; IDA:PAMGO_GAT.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0051170; P:import into nucleus; IDA:PAMGO_GAT.
DR   InterPro; IPR009933; VirD1.
DR   Pfam; PF07328; VirD1; 1.
PE   1: Evidence at protein level;
KW   Crown gall tumor; Endonuclease; Hydrolase; Nuclease; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..147
FT                   /note="T-DNA border endonuclease VirD1"
FT                   /id="PRO_0000065856"
SQ   SEQUENCE   147 AA;  16130 MW;  5C97C065BB7B60F1 CRC64;
     MSQGSRPTSS DIAVNQRECV KVEGFKVVST RLRSAEYESF SHQARLLGLS DSMAIRVAVR
     RIGGFLEIDA ETRHRMEAIL QSIGTLSSNI AALLSAYAEN PTMDLEALRA ERIAFGKSFA
     DLDGLLRSIL SVSRRRIDGC SLLKDAL