ID   VIRD1_RHIRD             Reviewed;         147 AA.
AC   P06667;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=T-DNA border endonuclease VirD1;
DE            EC=3.1.-.-;
GN   Name=virD1;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6NC.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3021341; DOI=10.1016/0092-8674(86)90604-5;
RA   Yanofsky M.F., Porter S.G., Young C., Albright L.M., Gordon M.P.,
RA   Nester E.W.;
RT   "The virD operon of Agrobacterium tumefaciens encodes a site-specific
RT   endonuclease.";
RL   Cell 47:471-477(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2822660; DOI=10.1128/jb.169.11.5035-5045.1987;
RA   Jayaswal R.K., Veluthambi K., Gelvin S.B., Slightom J.L.;
RT   "Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA
RT   molecules in Escherichia coli by a virD-encoded border-specific
RT   endonuclease from Agrobacterium tumefaciens.";
RL   J. Bacteriol. 169:5035-5045(1987).
CC   -!- FUNCTION: Tumor formation by A.tumefaciens involves the transfer and
CC       integration of a defined segment (T-DNA) of Ti plasmid DNA into the
CC       plant nuclear genome. The virD operon encodes a site-specific
CC       endonuclease that cleaves at a unique site within both 24 bp direct
CC       repeats flanking the T-DNA.
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DR   EMBL; AF242881; AAA98389.1; -; Genomic_DNA.
DR   EMBL; M17989; AAA22113.1; -; Genomic_DNA.
DR   PIR; A29826; A25063.
DR   RefSeq; NP_059813.1; NC_002377.1.
DR   RefSeq; WP_010892501.1; NZ_QSNU01000012.1.
DR   AlphaFoldDB; P06667; -.
DR   OrthoDB; 1555597at2; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR009933; VirD1.
DR   Pfam; PF07328; VirD1; 1.
PE   4: Predicted;
KW   Crown gall tumor; Endonuclease; Hydrolase; Nuclease; Plasmid.
FT   CHAIN           1..147
FT                   /note="T-DNA border endonuclease VirD1"
FT                   /id="PRO_0000065857"
SQ   SEQUENCE   147 AA;  16216 MW;  5321069D2D439E6E CRC64;
     MSKHTRVTSS ETAINQHRSL NVEGFKVVSA RLRSAEYETF SYQARLLGLS DSMAIRVAVR
     RIGGFLEIDA HTREKMEAIL QSIGILSSNV SMLLSAYAED PRSDLEAVRD ERIAFGEAFA
     ALDGLLRSIL SVSRRRIDGC SLLKGAL