VIRD2_AGRFC
ID VIRD2_AGRFC Reviewed; 447 AA.
AC P18592; P06521; P06522;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=T-DNA border endonuclease VirD2;
DE EC=3.1.-.-;
GN Name=virD2; OrderedLocusNames=Atu6182; ORFNames=AGR_pTi_21;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2986128; DOI=10.1073/pnas.82.9.2669;
RA Hagiya M., Close T.J., Tait R.C., Kado C.I.;
RT "Identification of pTiC58 plasmid-encoded proteins for virulence in
RT Agrobacterium tumefaciens.";
RL Proc. Natl. Acad. Sci. U.S.A. 82:2669-2673(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: Tumor formation by A.tumefaciens involves the transfer and
CC integration of a defined segment (T-DNA) of Ti plasmid DNA into the
CC plant nuclear genome. The virD operon encodes a site-specific
CC endonuclease that cleaves at a unique site within both 24 bp direct
CC repeats flanking the T-DNA.
CC -!- INTERACTION:
CC P18592; P07165: virC1; NbExp=4; IntAct=EBI-7412635, EBI-7412683;
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M11311; AAA98366.1; ALT_SEQ; Genomic_DNA.
DR EMBL; J03320; AAA91604.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90944.1; -; Genomic_DNA.
DR PIR; AD3250; AD3250.
DR PIR; B22666; B22666.
DR PIR; S11839; S11839.
DR RefSeq; NP_396503.1; NC_003065.3.
DR RefSeq; WP_010974919.1; NC_003065.3.
DR PDB; 4BQK; X-ray; 2.00 A; C/D=415-434.
DR PDBsum; 4BQK; -.
DR AlphaFoldDB; P18592; -.
DR SMR; P18592; -.
DR IntAct; P18592; 2.
DR MINT; P18592; -.
DR EnsemblBacteria; AAK90944; AAK90944; Atu6182.
DR KEGG; atu:Atu6182; -.
DR PATRIC; fig|176299.10.peg.5376; -.
DR HOGENOM; CLU_663697_0_0_5; -.
DR OMA; EWAAEMF; -.
DR BioCyc; AGRO:ATU6182-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0003677; F:DNA binding; IDA:PAMGO_GAT.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0051170; P:import into nucleus; IDA:PAMGO_GAT.
DR GO; GO:0051819; P:induction by symbiont of tumor or growth in host; IEA:InterPro.
DR InterPro; IPR005094; Endonuclease_MobA/VirD2.
DR InterPro; IPR016644; VirD2.
DR Pfam; PF03432; Relaxase; 1.
DR PIRSF; PIRSF016095; Endonuclease_VirD2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Crown gall tumor; Endonuclease; Hydrolase; Nuclease; Plasmid;
KW Reference proteome.
FT CHAIN 1..447
FT /note="T-DNA border endonuclease VirD2"
FT /id="PRO_0000065859"
FT REGION 214..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..447
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 250..264
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 327..350
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 367..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 414..447
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 12
FT /note="V -> M (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 447 AA; 49548 MW; 4BDB702F2EE894AC CRC64;
MPDRAQVIIR IVPGGGTKTL QQIINQLEYL SRKGRLELQR SARHLDIPLP PDQIHELARS
WVQETGTYDE SQPDEERQQE LTTHIIVSFP AGTSQVAAYA ASREWAAEMF GSGAGGGRYN
YLTAFHIDRD HPHLHVVVNR RELLGHGWLK ISRRHPQLNY DALRIKMAEI SLRHGIALDA
SRRAERGITE RPITYAQYRR LEREQARQIR FEDADLEQSS PQGDHPEFSQ PFDTSPFEAS
AGGPEDMPRP NNRQNESQVH LQEPAGVSNE AGVLVRVALE TERLAQPFVS ETILADDIGS
GSSRVAEGRV ESANRTPDIP RAATEAATHT THDRQRRAKR PHDDDGGPSG AKRVTLEGIA
VGPQANAGEQ DGSSGPLVRQ AGTSRPSPPT ATTRASTATD SLSATAHLQQ RRGVLSKRPR
EDDDGEPSER KRERDERSKD GRGGNRR
