VIRD2_RHIRD
ID VIRD2_RHIRD Reviewed; 424 AA.
AC P06668;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=T-DNA border endonuclease VirD2;
DE EC=3.1.-.-;
GN Name=virD2;
OS Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS radiobacter).
OG Plasmid pTiA6NC.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=358;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3021341; DOI=10.1016/0092-8674(86)90604-5;
RA Yanofsky M.F., Porter S.G., Young C., Albright L.M., Gordon M.P.,
RA Nester E.W.;
RT "The virD operon of Agrobacterium tumefaciens encodes a site-specific
RT endonuclease.";
RL Cell 47:471-477(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2822660; DOI=10.1128/jb.169.11.5035-5045.1987;
RA Jayaswal R.K., Veluthambi K., Gelvin S.B., Slightom J.L.;
RT "Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA
RT molecules in Escherichia coli by a virD-encoded border-specific
RT endonuclease from Agrobacterium tumefaciens.";
RL J. Bacteriol. 169:5035-5045(1987).
CC -!- FUNCTION: Tumor formation by A.tumefaciens involves the transfer and
CC integration of a defined segment (T-DNA) of Ti plasmid DNA into the
CC plant nuclear genome. The virD operon encodes a site-specific
CC endonuclease that cleaves at a unique site within both 24 bp direct
CC repeats flanking the T-DNA.
CC -!- INTERACTION:
CC P06668; Q7D3V8: Atu5117; Xeno; NbExp=3; IntAct=EBI-15673566, EBI-15673556;
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DR EMBL; AF242881; AAA98390.1; -; Genomic_DNA.
DR EMBL; M17989; AAA22114.1; -; Genomic_DNA.
DR PIR; B29826; B25063.
DR RefSeq; NP_059814.1; NC_002377.1.
DR RefSeq; WP_010892502.1; NZ_QSNU01000012.1.
DR AlphaFoldDB; P06668; -.
DR DIP; DIP-29956N; -.
DR ELM; P06668; -.
DR IntAct; P06668; 1.
DR OrthoDB; 358435at2; -.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0051819; P:induction by symbiont of tumor or growth in host; IEA:InterPro.
DR InterPro; IPR005094; Endonuclease_MobA/VirD2.
DR InterPro; IPR016644; VirD2.
DR Pfam; PF03432; Relaxase; 1.
DR PIRSF; PIRSF016095; Endonuclease_VirD2; 1.
PE 1: Evidence at protein level;
KW Crown gall tumor; Endonuclease; Hydrolase; Nuclease; Plasmid.
FT CHAIN 1..424
FT /note="T-DNA border endonuclease VirD2"
FT /id="PRO_0000065860"
FT REGION 206..269
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..424
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..232
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 239..258
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 393..424
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 424 AA; 47546 MW; B2CB7B82C01A6C1C CRC64;
MPDRAQVIIR IVPGGGTKTL QQIINQLEYL SRKGKLELQR SARHLDIPVP PDQIRELAQS
WVTEAGIYDE SQSDDDRQQD LTTHIIVSFP AGTDQTAAYE ASREWAAEMF GSGYGGGRYN
YLTAYHVDRD HPHLHVVVNR RELLGHGWLK ISRRHPQLNY DGLRKKMAEI SLRHGIVLDA
TSRAERGIAE RPITYAEHRR LERMQAQKIQ FEDTDFDETS PEEDRRDLSQ SFDPFRSDPS
TGEPDRATRH DKQPLEQHAR FQESAGSSIK ADARIRVSLE SERSAQPSAS KIPVIGHFGI
ETSYVAEASV RKRSGIFGTS RPVTDVAMHT VKRQQRSKRR NDEEAGPSGA NRKGLKAAQV
DSEANVGEQD TRDDSNKAAD PVSASIGTEQ PEASPKRPRD RHDGELGGRK RARGNRRDDG
RGGT
