VIRD4_AGRFC
ID VIRD4_AGRFC Reviewed; 668 AA.
AC P18594;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Protein VirD4;
GN Name=virD4; OrderedLocusNames=Atu6184; ORFNames=AGR_pTi_23;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- INTERACTION:
CC P18594; P07165: virC1; NbExp=4; IntAct=EBI-7412695, EBI-7412683;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
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DR EMBL; J03320; AAA91606.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90946.2; -; Genomic_DNA.
DR PIR; AF3250; AF3250.
DR PIR; S11841; S11841.
DR RefSeq; NP_396505.2; NC_003065.3.
DR RefSeq; WP_010974920.1; NC_003065.3.
DR AlphaFoldDB; P18594; -.
DR IntAct; P18594; 1.
DR MINT; P18594; -.
DR EnsemblBacteria; AAK90946; AAK90946; Atu6184.
DR KEGG; atu:Atu6184; -.
DR PATRIC; fig|176299.10.peg.5378; -.
DR HOGENOM; CLU_012039_1_3_5; -.
DR OMA; FIYEPFS; -.
DR PhylomeDB; P18594; -.
DR BioCyc; AGRO:ATU6184-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043684; C:type IV secretion system complex; TAS:PAMGO_GAT.
DR GO; GO:0030255; P:protein secretion by the type IV secretion system; TAS:PAMGO_GAT.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003688; TraG/VirD4.
DR Pfam; PF02534; T4SS-DNA_transf; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Conjugation; Crown gall tumor; Membrane; Plasmid;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..668
FT /note="Protein VirD4"
FT /id="PRO_0000221652"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 577..612
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 587..602
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 189
FT /note="G -> R (in Ref. 1; AAA91606)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="E -> D (in Ref. 1; AAA91606)"
FT /evidence="ECO:0000305"
FT CONFLICT 576..582
FT /note="QGLGDID -> ASTR (in Ref. 1; AAA91606)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 668 AA; 73528 MW; 376925564AE740DF CRC64;
MNSSKTTPQR LAVSIVCSLA AGFCAASLYV TFRHGFNGEA MMTFSVFAFW YETPLYMGHA
TPVFYCGLAI VVSTSIVVLL SQLIISFRNH EHHGTARWAG FGEMRHAGYL QRYNRIKGPI
FGKTCGPRWF GSYLTNGEQP HSLVVAPTRA GKGVGVVIPT LLTFKGSVIA LDVKGELFEL
TSRARKAGGD AVFKFSPLDP ERRTHCYNPV LDIAALPPER QFTETRRLAA NLITAKGKGA
EGFIDGARDL FVAGILTCIE RGTPTIGAVY DLFAQPGEKY KLFAHLAEES RNKEAQRIFD
NMAGNDTKIL TSYTSVLGDG GLNLWADPLV KAATSRSDFS VYDLRRKRTC VYLCVSPNDL
EVVAPLMRLL FQQVVSILQR SLPGKDERHE VLFLLDEFKH LGKLEAIETA ITTIAGYKGR
FMFIIQSLSA LTGIYDDAGK QNFLSNTGVQ VFMATADDET PTYISKAIGD YTFKARSTSY
SQARMFDHNI QISDQGAPLL RPEQVRLLDD NNEIVLIKGH PPLKLRKVRY YSDRMLRRLF
ECQIGALPEP ASLMLSEGVH RDGQDLSQQA AVTEAQGLGD IDSIPNNMEA ATPQNSEMDD
EQDSLPTGID VPQGLIESDE VKEDAGGVVP DFGVSAEMAP AMIAQQQLLE QIIALQQRYG
PASSHSVK
