ID   VIRD4_BARHE             Reviewed;         639 AA.
AC   Q6G2A8;
DT   23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Type IV secretion system-coupling protein VirD4;
GN   Name=virD4; Synonyms=traG; OrderedLocusNames=BH13380;
OS   Bartonella henselae (strain ATCC 49882 / DSM 28221 / Houston 1)
OS   (Rochalimaea henselae).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bartonellaceae; Bartonella.
OX   NCBI_TaxID=283166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15642951; DOI=10.1073/pnas.0406796102;
RA   Schulein R., Guye P., Rhomberg T.A., Schmid M.C., Schroeder G.,
RA   Vergunst A.C., Carena I., Dehio C.;
RT   "A bipartite signal mediates the transfer of type IV secretion substrates
RT   of Bartonella henselae into human cells.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:856-861(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49882 / DSM 28221 / Houston 1;
RX   PubMed=15210978; DOI=10.1073/pnas.0305659101;
RA   Alsmark U.C.M., Frank A.C., Karlberg E.O., Legault B.-A., Ardell D.H.,
RA   Canbaeck B., Eriksson A.-S., Naeslund A.K., Handley S.A., Huvet M.,
RA   La Scola B., Holmberg M., Andersson S.G.E.;
RT   "The louse-borne human pathogen Bartonella quintana is a genomic derivative
RT   of the zoonotic agent Bartonella henselae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:9716-9721(2004).
CC   -!- FUNCTION: The type IV secretion system VirB/VirD4 is a major virulence
CC       determinant for subversion of human endothelial cell (HEC) function.
CC       VirB-dependent changes of HEC include massive cytoskeletal
CC       rearrangements, a pro-inflammatory activation by nuclear factor NF-
CC       kappa-B, inhibition of early and late events of apoptosis, leading to
CC       an increased cell survival, and, at high infection doses, a cytostatic
CC       or cytotoxic effect, which interfers with a potent VirB-independent
CC       mitogenic activity. These changes of HEC require the T4S coupling
CC       protein VirD4 and at least one of the effector proteins BepA-G.
CC       {ECO:0000269|PubMed:15642951}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AJ556988; CAD89507.1; -; Genomic_DNA.
DR   EMBL; BX897699; CAF28111.1; -; Genomic_DNA.
DR   RefSeq; WP_011181139.1; NZ_LRIJ02000001.1.
DR   AlphaFoldDB; Q6G2A8; -.
DR   IntAct; Q6G2A8; 1.
DR   STRING; 283166.BH13380; -.
DR   PaxDb; Q6G2A8; -.
DR   DNASU; 2865237; -.
DR   EnsemblBacteria; CAF28111; CAF28111; BH13380.
DR   KEGG; bhe:BH13380; -.
DR   eggNOG; COG3505; Bacteria.
DR   OMA; SYFQNQA; -.
DR   Proteomes; UP000000421; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014135; Ti-typ_conjug_TS_TraG-like.
DR   InterPro; IPR003688; TraG/VirD4.
DR   Pfam; PF02534; T4SS-DNA_transf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR02767; TraG-Ti; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport;
KW   Virulence.
FT   CHAIN           1..639
FT                   /note="Type IV secretion system-coupling protein VirD4"
FT                   /id="PRO_0000273257"
FT   TRANSMEM        13..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        49..69
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        118..138
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   639 AA;  71447 MW;  FF9271EFAC82CAC7 CRC64;
     MKYTKTQLAL ISMPIASGAL TIFLVPHMLS FVINDLKTNQ IYWYVRSEPL LTLMLVAAVS
     LFYTLSQKLH LRKAITFVST AFFCITALYY IGSEIKRLNP YVGQQGITWG YALKFMDPMV
     VFGVILGFVL LAIQVIITSP RTSNVKRAKK GIFGDAAWMN LKEAARIFPS NGQIVIGERY
     RVDQDNVRNI PFAPGNKTTW GKGGTAPLLT FNLDFGSTHM IFFAGSGGYK TTSTVVPTCL
     TYTGPIVCLD PSTEIAPMVK FARKKMGNRN VIILDPNSLL TKNFNVLDWL LDENIPRTRR
     EANIVSFSKL LLSEKKSENS SAEYFSTQAH NLLTALLAHV IFSDKYEDSE RNLKTLRAIL
     SQSETAVVNQ LRMIQETTPS PFIREMVGIF TEMADQTFSG VYTTASKDTQ WLSLSNYADL
     VCGNDFASSD IANGKTDVFL NLPASILNSY PAIGRVIIGA FLNAMVTADG NYKKRVLFVL
     DEVDLLGYMN ILEEARDRGR KYGTSLMLFY QSSGQLVNHF GEAGARSWFE SCSFVSYAAI
     KDLQTAKDIS ERCGQMTIEV TGTSKSRGLS LTKGSQNINY QQRALILPHE IIQEMRQDEQ
     IILMQGHPPL RCGRAIYFRR KEMLAATEKN RFAPQAKKS