ID   VIRD4_RHIRD             Reviewed;         688 AA.
AC   P09817; P13465;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Protein VirD4;
GN   Name=virD4;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6NC.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3658701; DOI=10.1093/nar/15.18.7503;
RA   Porter S.G., Yanofsky M.F., Nester E.W.;
RT   "Molecular characterization of the virD operon from Agrobacterium
RT   tumefaciens.";
RL   Nucleic Acids Res. 15:7503-7517(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2822660; DOI=10.1128/jb.169.11.5035-5045.1987;
RA   Jayaswal R.K., Veluthambi K., Gelvin S.B., Slightom J.L.;
RT   "Double-stranded cleavage of T-DNA and generation of single-stranded T-DNA
RT   molecules in Escherichia coli by a virD-encoded border-specific
RT   endonuclease from Agrobacterium tumefaciens.";
RL   J. Bacteriol. 169:5035-5045(1987).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the VirD4/TraG family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA22117.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X06045; CAA29438.1; -; Genomic_DNA.
DR   EMBL; M17989; AAA22117.1; ALT_FRAME; Genomic_DNA.
DR   RefSeq; NP_059816.1; NC_002377.1.
DR   AlphaFoldDB; P09817; -.
DR   DIP; DIP-29959N; -.
DR   IntAct; P09817; 2.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR003688; TraG/VirD4.
DR   Pfam; PF02534; T4SS-DNA_transf; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Conjugation; Crown gall tumor; Membrane; Plasmid;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..688
FT                   /note="Protein VirD4"
FT                   /id="PRO_0000221653"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        67..87
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   688 AA;  75950 MW;  43BF81126FFC3C46 CRC64;
     MNSSKTSPQR MTLSIVCSLA AGFCAASCYV TFRRGFNGEA IMTFDVFAFW YETPLYLGYA
     STVFWRGLSV VIFTSLAVLS SQLIISLRNQ KHHGTARWAE IGEMQHAGYL QRYSRIKGPI
     FGKTCGPLWF GSYLTNGEQP HSLVVAPTRA GKGVGIVIPT LLTFKGSVIA LDVKGELFEL
     TSRARKASGD AVFKFSPLDS ERKTHCYNPV LDIATLPPER QFTETRRLAA NLITAKGKGA
     EGFIDGARDL FVAGILTCIE RGTPTIGAVY DLFAQPGEKY KLFAQLAEES LNKEAQRIFD
     NMAGNDTKIL TSYTSVLGDG GLNLWADPLI KAATSRSDFS VYDLRRKKTC IYLCVSPNDL
     EVLAPLMRLL FQQLVSILQR SLPGKDECHE VLFLLDEFKH LGKLEAIETA ITTIAGYKGR
     FMFIIQSLSA LTGTYDDAGK QNFLSNTGVQ VFMATADDET PTYISKAIGE YTFKARSTSY
     SQASMFDHNI QISDQGAALL RPEQVRLLDD QSEIVLIKGR PPLKLRKVQY YSDRTLKRLF
     ERQMGSLPEP APLMLSDYSN DQVQSHLAEI ANFNEDAAPR NRTVAEDHGS VKVGADIPER
     VMGINGDEDQ ADAREIPPEL GCASRIDAGS GRSTAIVGPD YCTSAKIEVR TGTACEMKLN
     WVQFVSGGML RFDKVYARSQ LSLYEVSV