VIRE2_AGRFC
ID VIRE2_AGRFC Reviewed; 556 AA.
AC P08062;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Single-strand DNA-binding protein;
DE AltName: Full=63.5 kDa virulence protein;
GN Name=virE2; OrderedLocusNames=Atu6190; ORFNames=AGR_pTi_28;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3549694; DOI=10.1128/jb.169.4.1529-1536.1987;
RA Hirooka T., Rogowsky P.M., Kado C.I.;
RT "Characterization of the virE locus of Agrobacterium tumefaciens plasmid
RT pTiC58.";
RL J. Bacteriol. 169:1529-1536(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
RN [5]
RP FUNCTION.
RX PubMed=17784072; DOI=10.1126/science.240.4851.501;
RA Citovsky V., de Vos G., Zambryski P.;
RT "Single-stranded DNA binding protein encoded by the virE locus of
RT Agrobacterium tumefaciens.";
RL Science 240:501-504(1988).
RN [6]
RP FUNCTION.
RX PubMed=8637884; DOI=10.1073/pnas.93.6.2392;
RA Zupan J.R., Citovsky V., Zambryski P.;
RT "Agrobacterium VirE2 protein mediates nuclear uptake of single-stranded DNA
RT in plant cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:2392-2397(1996).
RN [7]
RP SUBCELLULAR LOCATION.
RX PubMed=1615325; DOI=10.1126/science.1615325;
RA Citovsky V., Zupan J., Warnick D., Zambryski P.;
RT "Nuclear localization of Agrobacterium VirE2 protein in plant cells.";
RL Science 256:1802-1805(1992).
RN [8]
RP FUNCTION, AND INTERACTION WITH ARABIDOPSIS VIP1.
RX PubMed=12124400; DOI=10.1073/pnas.162304099;
RA Tzfira T., Vaidya M., Citovsky V.;
RT "Increasing plant susceptibility to Agrobacterium infection by
RT overexpression of the Arabidopsis nuclear protein VIP1.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:10435-10440(2002).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH VIRE1.
RX PubMed=18678909; DOI=10.1073/pnas.0801525105;
RA Dym O., Albeck S., Unger T., Jacobovitch J., Branzburg A., Michael Y.,
RA Frenkiel-Krispin D., Wolf S.G., Elbaum M.;
RT "Crystal structure of the Agrobacterium virulence complex VirE1-VirE2
RT reveals a flexible protein that can accommodate different partners.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:11170-11175(2008).
CC -!- FUNCTION: Involved in DNA transformation; mediates the nuclear uptake
CC of single-stranded DNA copies of the transferred DNA (T-DNA) element.
CC Binds single-stranded but not double-stranded DNA regardless of
CC nucleotide sequence composition. {ECO:0000269|PubMed:12124400,
CC ECO:0000269|PubMed:17784072, ECO:0000269|PubMed:8637884}.
CC -!- SUBUNIT: Interacts with host VIP2 that promotes T-DNA integration into
CC the host genome. Forms a complex made of virE2 and host proteins VIP1
CC and VBF (By similarity). Forms heterodimers with the chaperone protein
CC virE1 that prevent virE2 anarchic homopolymerization. Interacts with
CC A.thaliana VIP1 that mediates its translocation to the host nucleus.
CC Forms a complex made of VirE2, host VIP1 and VIP2 and single-stranded
CC DNA (ssDNA). {ECO:0000250, ECO:0000269|PubMed:12124400,
CC ECO:0000269|PubMed:18678909}.
CC -!- INTERACTION:
CC P08062; P08062: virE2; NbExp=3; IntAct=EBI-6402090, EBI-6402090;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1615325}. Host
CC nucleus {ECO:0000250}. Note=In infected cells, it is found in the
CC nucleus.
CC -!- INDUCTION: Targeted to degradation by the host proteasome by VBF and
CC Agrobacterium virF in SCF(VBF) and SCF(COI1) E3 ubiquitin ligase
CC complexes after mediating T-DNA translocation to the nucleus.
CC {ECO:0000250}.
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DR EMBL; M15814; AAA98372.1; -; Genomic_DNA.
DR EMBL; J03320; AAA91610.1; -; Genomic_DNA.
DR EMBL; AE007871; AAK90951.1; -; Genomic_DNA.
DR PIR; A26939; A26939.
DR PIR; AD3251; AD3251.
DR RefSeq; NP_396510.1; NC_003065.3.
DR RefSeq; WP_010974922.1; NC_003065.3.
DR PDB; 3BTP; X-ray; 2.30 A; A=1-556.
DR PDB; 4BLF; EM; 20.00 A; A=112-337.
DR PDBsum; 3BTP; -.
DR PDBsum; 4BLF; -.
DR AlphaFoldDB; P08062; -.
DR SMR; P08062; -.
DR DIP; DIP-46168N; -.
DR IntAct; P08062; 2.
DR EnsemblBacteria; AAK90951; AAK90951; Atu6190.
DR KEGG; atu:Atu6190; -.
DR PATRIC; fig|176299.10.peg.5380; -.
DR HOGENOM; CLU_510604_0_0_5; -.
DR OMA; AEFNASC; -.
DR BioCyc; AGRO:ATU6190-MON; -.
DR EvolutionaryTrace; P08062; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0009294; P:DNA-mediated transformation; IDA:UniProtKB.
DR InterPro; IPR009868; VirE2.
DR Pfam; PF07229; VirE2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Crown gall tumor; DNA-binding; Host nucleus; Plasmid;
KW Reference proteome; Secreted; Virulence.
FT CHAIN 1..556
FT /note="Single-strand DNA-binding protein"
FT /id="PRO_0000065866"
FT REGION 1..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 527..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 32..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..72
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 77..95
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 282
FT /note="A -> R (in Ref. 1; AAA98372 and 2; AAA91610)"
FT /evidence="ECO:0000305"
FT STRAND 116..124
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 127..132
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 142..147
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 148..153
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 162..167
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 173..177
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 186..206
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 231..233
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 234..237
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 239..241
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 243..245
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 250..252
FT /evidence="ECO:0007829|PDB:3BTP"
FT TURN 253..259
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 262..266
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 271..275
FT /evidence="ECO:0007829|PDB:3BTP"
FT TURN 282..285
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 288..299
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 304..308
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 314..316
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 317..323
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 327..335
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 348..352
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 367..370
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 371..377
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 382..388
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 393..395
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 396..399
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 403..407
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 409..415
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 417..427
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 431..435
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 452..456
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 458..462
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 464..466
FT /evidence="ECO:0007829|PDB:3BTP"
FT TURN 469..471
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 480..485
FT /evidence="ECO:0007829|PDB:3BTP"
FT STRAND 491..497
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 498..503
FT /evidence="ECO:0007829|PDB:3BTP"
FT HELIX 510..515
FT /evidence="ECO:0007829|PDB:3BTP"
SQ SEQUENCE 556 AA; 63345 MW; 73C267DE9F2101B6 CRC64;
MDPKAEGNGE NITETAAGNV ETSDFVNLKR QKREGVNSTG MSEIDMTGSQ ETPEHNMHGS
PTHTDDLGPR LDADMLDSQS SHVSSSAQGN RSEVENELSN LFAKMALPGH DRRTDEYILV
RQTGQDKFAG TTKCNLDHLP TKAEFNASCR LYRDGVGNYY PPPLAFERID IPEQLAAQLH
NLEPREQSKQ CFQYKLEVWN RAHAEMGITG TDIFYQTDKN IKLDRNYKLR PEDRYIQTEK
YGRREIQKRY EHQFQAGSLL PDILIKTPQN DIHFSYRFAG DAYANKRFEE FERAIKTKYG
SDTEIKLKSK SGIMHDSKYL ESWERGSADI RFAEFAGENR AHNKQFPAAT VNMGRQPDGQ
GGMTRDRHVS VDYLLQNLPN SPWTQALKEG KLWDRVQVLA RDGNRYMSPS RLEYSDPEHF
TQLMDQVGLP VSMGRQSHAN SVKFEQFDRQ AAVIVADGPN LREVPDLSPE KLQQLSQKDV
LIADRNEKGQ RTGTYTNVVE YERLMMKLPS DAAQLLAEPS DRYSRAFVRP EPALPPISDS
RRTYESRPRG PTVNSL
