ID   VIRE2_AGRT9             Reviewed;         533 AA.
AC   P0A3W9; P07544; Q9JN10;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Single-strand DNA-binding protein;
GN   Name=virE2;
OS   Agrobacterium tumefaciens (strain 15955).
OG   Plasmid pTi15955.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=190386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10948245; DOI=10.1093/jexbot/51.347.1167;
RA   Schrammeijer B., Beijersbergen A., Idler K.B., Melchers L.S.,
RA   Thompson D.V., Hooykaas P.J.J.;
RT   "Sequence analysis of the vir-region from Agrobacterium tumefaciens
RT   octopine Ti plasmid pTi15955.";
RL   J. Exp. Bot. 51:1167-1169(2000).
CC   -!- FUNCTION: Involved in DNA transformation; mediates the nuclear uptake
CC       of single-stranded DNA copies of the transferred DNA (T-DNA) element.
CC       Binds single-stranded but not double-stranded DNA regardless of
CC       nucleotide sequence composition (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Forms heterodimers with the chaperone protein virE1 that
CC       prevent virE2 anarchic homopolymerization. Interacts with host VIP1
CC       that mediates its translocation to the host nucleus and host VIP2 that
CC       promotes T-DNA integration into the host genome. Forms a complex made
CC       of VirE2, host VIP1 and VIP2 and single-stranded DNA (ssDNA). Forms a
CC       complex made of virE2 and host proteins VIP1 and VBF (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host nucleus
CC       {ECO:0000250}. Note=In infected cells, it is found in the nucleus.
CC       {ECO:0000250}.
CC   -!- INDUCTION: Targeted to degradation by the host proteasome by VBF and
CC       Agrobacterium virF in SCF(VBF) and SCF(COI1) E3 ubiquitin ligase
CC       complexes after mediating T-DNA translocation to the nucleus.
CC       {ECO:0000250}.
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DR   EMBL; X06826; CAC15175.1; -; Genomic_DNA.
DR   RefSeq; NP_059819.1; NC_002377.1.
DR   AlphaFoldDB; P0A3W9; -.
DR   SMR; P0A3W9; -.
DR   IntAct; P0A3W9; 1.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042025; C:host cell nucleus; ISS:UniProtKB.
DR   GO; GO:0003677; F:DNA binding; ISS:UniProtKB.
DR   GO; GO:0009294; P:DNA-mediated transformation; ISS:UniProtKB.
DR   InterPro; IPR009868; VirE2.
DR   Pfam; PF07229; VirE2; 1.
PE   3: Inferred from homology;
KW   Crown gall tumor; DNA-binding; Host nucleus; Plasmid; Secreted; Virulence.
FT   CHAIN           1..533
FT                   /note="Single-strand DNA-binding protein"
FT                   /id="PRO_0000065867"
FT   REGION          1..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          504..533
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        11..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        50..67
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   533 AA;  60587 MW;  D789196DD7218E1F CRC64;
     MDLSGNEKSR PWKKANVSSS TISDIQMTNG ENLESGSPTR TEVLSPRLDD GSVDSSSSLY
     SGSEHGNQAE IQKELSALFS NMSLPGNDRR PDEYILVRQT GQDAFTGIAK GNLDHMPTKA
     EFNACCRLYR DGAGNYYPPP LAFDKISVPA QLEETWGMME AKERNKLRFQ YKLDVWNHAH
     ADMGITGTEI FYQTDKNIKL DRNYKLRPED RYVQTERYGR REIQKRYQHE LQAGSLLPDI
     MIKTPKNDIH FVYRFAGDNY ANKQFSEFEH TVKRRYGGET EIKLKSKSGI MHDSKYLESW
     ERGSADIRFA EFVGENRAHN RQFPTATVNM GQQPDGQGGL TRDRHVSVEF LMQSAPNSPW
     AQALKKGELW DRVQLLARDG NRYLSPHRLE YSDPEHFTEL MNRVGLPASM GRQSHAASIK
     FEKFDAQAAV IVINGPELRD IHDLSPENLQ NVSTKDVIVA DRNENGQRTG TYTSVAEYER
     LQLRLPADAA GVLGEAADKY SRDFVRPEPA SRPISDSRRI YESRPRSQSV NSF