ID   VIRF_AGRT9              Reviewed;         202 AA.
AC   P15597;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Virulence protein F {ECO:0000303|PubMed:2101693};
GN   Name=virF {ECO:0000303|PubMed:2101693};
OS   Agrobacterium tumefaciens (strain 15955).
OG   Plasmid pTi15955.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=190386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2101693; DOI=10.1007/bf00018565;
RA   Melchers L.S., Maroney M.J., den Dulk-Ras A., Thompson D.V.,
RA   van Vuuren H.A.J., Schilperoort R.A., Hooykaas P.J.J.;
RT   "Octopine and nopaline strains of Agrobacterium tumefaciens differ in
RT   virulence; molecular characterization of the virF locus.";
RL   Plant Mol. Biol. 14:249-259(1990).
RN   [2]
RP   INTERACTION WITH ARABIDOPSIS THALIANA ENAP1/VFP3 AND VFP5, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=26571494; DOI=10.1371/journal.pone.0142128;
RA   Garcia-Cano E., Magori S., Sun Q., Ding Z., Lazarowitz S.G., Citovsky V.;
RT   "Interaction of Arabidopsis trihelix-domain transcription factors VFP3 and
RT   VFP5 with Agrobacterium virulence protein VirF.";
RL   PLoS ONE 10:e0142128-e0142128(2015).
CC   -!- FUNCTION: In the host plant, component of SCF(virF) E3 ubiquitin ligase
CC       complexes, which mediate the ubiquitination and subsequent proteasomal
CC       degradation of target proteins such as the host VIP1, after its
CC       implication in T-DNA translocation to the host nucleus (By similarity).
CC       Required for the formation of tumors of a wild-type size on certain
CC       plant species only. {ECO:0000250}.
CC   -!- SUBUNIT: Component of SCF(virF) E3 ubiquitin ligase complexes.
CC       Interacts with host VIP1 and SKP1A (By similarity). Interacts with
CC       Arabidopsis thaliana ENAP1/VFP3 and VFP5 in the host cell nucleus
CC       (PubMed:26571494). {ECO:0000250, ECO:0000269|PubMed:26571494}.
CC   -!- INTERACTION:
CC       P15597; Q9C5T5: ASK10; Xeno; NbExp=4; IntAct=EBI-605118, EBI-607786;
CC       P15597; Q39255: SKP1A; Xeno; NbExp=6; IntAct=EBI-605118, EBI-532357;
CC       P15597; Q9FHW7: SKP1B; Xeno; NbExp=5; IntAct=EBI-605118, EBI-604076;
CC       P15597; Q9MA75: VIP1; Xeno; NbExp=3; IntAct=EBI-605118, EBI-606057;
CC   -!- SUBCELLULAR LOCATION: Host nucleus {ECO:0000269|PubMed:26571494}.
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DR   EMBL; X13981; CAA32163.1; -; Genomic_DNA.
DR   PIR; S15915; S07979.
DR   RefSeq; NP_059824.1; NC_002377.1.
DR   AlphaFoldDB; P15597; -.
DR   IntAct; P15597; 8.
DR   PRIDE; P15597; -.
DR   GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Crown gall tumor; Host nucleus; Plasmid; Ubl conjugation pathway;
KW   Virulence.
FT   CHAIN           1..202
FT                   /note="Virulence protein F"
FT                   /id="PRO_0000065870"
FT   DOMAIN          20..42
FT                   /note="F-box"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..19
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   202 AA;  22437 MW;  3B0E33E157C1ADD1 CRC64;
     MRNSSLRDAS GSNDAQVPHK TELLNLPDHV LTEVAKRLAT NNPVESAENI ANFSKSHRFT
     RDAVRTEPLE KFSSRLKILS RNAKLLSHAV RHAATLPDGE QLSEAQLSQM RSEVATRPVL
     GVAYTHQDGQ PEERLSGNHL DHKINNIPNL VFNVAEPIMF NEISALEVMA EVRPIARSIK
     TAHDDARAEL MSADRPRSTR GL