VIRF_SHIFL
ID VIRF_SHIFL Reviewed; 262 AA.
AC P0A2T1; Q04248; Q9AFW5;
DT 01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2005, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Virulence regulon transcriptional activator VirF;
GN Name=virF; OrderedLocusNames=CP0046;
OS Shigella flexneri.
OG Plasmid pWR100, Plasmid pMYSH6000, Plasmid pINV_F6_M1382, and
OG Plasmid pCP301.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=3021627; DOI=10.1128/iai.54.2.395-402.1986;
RA Sakai T., Sasakawa C., Makino S., Yoshikawa M.;
RT "DNA sequence and product analysis of the virF locus responsible for congo
RT red binding and cell invasion in Shigella flexneri 2a.";
RL Infect. Immun. 54:395-402(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11115111; DOI=10.1046/j.1365-2958.2000.02179.x;
RA Buchrieser C., Glaser P., Rusniok C., Nedjari H., d'Hauteville H.,
RA Kunst F., Sansonetti P.J., Parsot C.;
RT "The virulence plasmid pWR100 and the repertoire of proteins secreted by
RT the type III secretion apparatus of Shigella flexneri.";
RL Mol. Microbiol. 38:760-771(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M90T / Serotype 5a; PLASMID=pWR100;
RX PubMed=11292750; DOI=10.1128/iai.69.5.3271-3285.2001;
RA Venkatesan M.M., Goldberg M.B., Rose D.J., Grotbeck E.J., Burland V.,
RA Blattner F.R.;
RT "Complete DNA sequence and analysis of the large virulence plasmid of
RT Shigella flexneri.";
RL Infect. Immun. 69:3271-3285(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=M1382 / Serotype 6; PLASMID=pINV_F6_M1382;
RX PubMed=14573649; DOI=10.1128/iai.71.11.6298-6306.2003;
RA Lan R., Stevenson G., Reeves P.R.;
RT "Comparison of two major forms of the Shigella virulence plasmid pINV:
RT positive selection is a major force driving the divergence.";
RL Infect. Immun. 71:6298-6306(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a; PLASMID=pCP301;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [6]
RP SIMILARITY TO ARAC FAMILY.
RX PubMed=1625585; DOI=10.1111/j.1365-2958.1992.tb00879.x;
RA Dorman C.J.;
RT "The VirF protein from Shigella flexneri is a member of the AraC
RT transcription factor superfamily and is highly homologous to Rns, a
RT positive regulator of virulence genes in enterotoxigenic Escherichia
RT coli.";
RL Mol. Microbiol. 6:1575-1575(1992).
RN [7]
RP FUNCTION.
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=2474742; DOI=10.1111/j.1365-2958.1989.tb00210.x;
RA Adler B., Sasakawa C., Tobe T., Makino S., Komatsu K., Yoshikawa M.;
RT "A dual transcriptional activation system for the 230 kb plasmid genes
RT coding for virulence-associated antigens of Shigella flexneri.";
RL Mol. Microbiol. 3:627-635(1989).
RN [8]
RP FUNCTION.
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=7691791; DOI=10.1128/jb.175.19.6142-6149.1993;
RA Tobe T., Yoshikawa M., Mizuno T., Sasakawa C.;
RT "Transcriptional control of the invasion regulatory gene virB of Shigella
RT flexneri: activation by virF and repression by H-NS.";
RL J. Bacteriol. 175:6142-6149(1993).
RN [9]
RP REGULATION.
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=9766205; DOI=10.1016/s0923-2508(97)83619-4;
RA Prosseda G., Fradiani P.A., Di Lorenzo M., Falconi M., Micheli G.,
RA Casalino M., Nicoletti M., Colonna B.;
RT "A role for H-NS in the regulation of the virF gene of Shigella and
RT enteroinvasive Escherichia coli.";
RL Res. Microbiol. 149:15-25(1998).
RN [10]
RP REGULATION.
RC STRAIN=YSH6000 / Serotype 2a; PLASMID=pMYSH6000;
RX PubMed=14756791; DOI=10.1046/j.1365-2958.2003.03848.x;
RA Prosseda G., Falconi M., Giangrossi M., Gualerzi C.O., Micheli G.,
RA Colonna B.;
RT "The virF promoter in Shigella: more than just a curved DNA stretch.";
RL Mol. Microbiol. 51:523-537(2004).
CC -!- FUNCTION: Primary regulator of plasmid-encoded virulence genes.
CC Activates the transcription of icsA (virG) and of virB, which is an
CC activator of the ipaABCD virulence regulon.
CC {ECO:0000269|PubMed:2474742, ECO:0000269|PubMed:7691791}.
CC -!- INDUCTION: Transcriptionally regulated by FIS at 37 degrees Celsius.
CC Subject to H-NS-mediated transcriptional repression below 32 degrees
CC Celsius and at low pH.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK18362.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M29172; AAA26545.1; -; Genomic_DNA.
DR EMBL; X16661; CAA34648.1; -; Genomic_DNA.
DR EMBL; AL391753; CAC05780.1; -; Genomic_DNA.
DR EMBL; AF348706; AAK18362.1; ALT_INIT; Genomic_DNA.
DR EMBL; AY206433; AAP78977.1; -; Genomic_DNA.
DR EMBL; AF386526; AAL72294.2; -; Genomic_DNA.
DR PIR; A47605; A47605.
DR RefSeq; NP_858179.2; NC_004851.1.
DR RefSeq; WP_005116773.1; NZ_WPGT01000232.1.
DR RefSeq; YP_009062462.1; NC_024996.1.
DR AlphaFoldDB; P0A2T1; -.
DR SMR; P0A2T1; -.
DR STRING; 198214.CP0046; -.
DR EnsemblBacteria; AAL72294; AAL72294; SF_p0046.
DR GeneID; 1238022; -.
DR GeneID; 58461125; -.
DR KEGG; sfl:CP0046; -.
DR PATRIC; fig|198214.7.peg.5287; -.
DR HOGENOM; CLU_000445_81_4_6; -.
DR OMA; FQISHRC; -.
DR Proteomes; UP000001006; Plasmid pCP301.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR018060; HTH_AraC.
DR InterPro; IPR018062; HTH_AraC-typ_CS.
DR InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR Pfam; PF12833; HTH_18; 1.
DR PRINTS; PR00032; HTHARAC.
DR SMART; SM00342; HTH_ARAC; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Plasmid; Reference proteome; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..262
FT /note="Virulence regulon transcriptional activator VirF"
FT /id="PRO_0000194594"
FT DOMAIN 161..258
FT /note="HTH araC/xylS-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 178..199
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT DNA_BIND 225..248
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ SEQUENCE 262 AA; 30550 MW; 3CB18EA52DE53443 CRC64;
MMDMGHKNKI DIKVRLHNYI ILYAKRCSMT VSSGNETLTI DEGQIAFIER NIQINVSIKK
SDSINPFEII SLDRNLLLSI IRIMEPIYSF QHSYSEEKRG LNKKIFLLSE EEVSIDLFKS
IKEMPFGKRK IYSLACLLSA VSDEEALYTS ISIASSLSFS DQIRKIVEKN IEKRWRLSDI
SNNLNLSEIA VRKRLESEKL TFQQILLDIR MHHAAKLLLN SQSYINDVSR LIGISSPSYF
IRKFNEYYGI TPKKFYLYHK KF
