ID   VIRF_SHISO              Reviewed;         262 AA.
AC   P0A2T3; Q04248; Q9AFW5;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Virulence regulon transcriptional activator VirF;
GN   Name=virF;
OS   Shigella sonnei.
OG   Plasmid pINV.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=624;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HW383;
RX   PubMed=2651305; DOI=10.1128/iai.57.5.1391-1398.1989;
RA   Kato J., Ito K., Nakamura A., Watanabe H.;
RT   "Cloning of regions required for contact hemolysis and entry into LLC-MK2
RT   cells from Shigella sonnei form I plasmid: virF is a positive regulator
RT   gene for these phenotypes.";
RL   Infect. Immun. 57:1391-1398(1989).
CC   -!- FUNCTION: Primary regulator of plasmid-encoded virulence genes.
CC       Activates the transcription of icsA (virG) and of virB, which is an
CC       activator of the ipaABCD virulence regulon (By similarity).
CC       {ECO:0000250}.
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DR   PIR; A60105; A60105.
DR   RefSeq; WP_005116773.1; NZ_UIQD01000296.1.
DR   AlphaFoldDB; P0A2T3; -.
DR   SMR; P0A2T3; -.
DR   STRING; 216599.GCA_000283715_05164; -.
DR   GeneID; 58461125; -.
DR   PATRIC; fig|624.635.peg.5076; -.
DR   OMA; FQISHRC; -.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR018060; HTH_AraC.
DR   InterPro; IPR018062; HTH_AraC-typ_CS.
DR   InterPro; IPR020449; Tscrpt_reg_HTH_AraC-type.
DR   Pfam; PF12833; HTH_18; 1.
DR   PRINTS; PR00032; HTHARAC.
DR   SMART; SM00342; HTH_ARAC; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00041; HTH_ARAC_FAMILY_1; 1.
DR   PROSITE; PS01124; HTH_ARAC_FAMILY_2; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Plasmid; Transcription; Transcription regulation;
KW   Virulence.
FT   CHAIN           1..262
FT                   /note="Virulence regulon transcriptional activator VirF"
FT                   /id="PRO_0000194595"
FT   DOMAIN          161..258
FT                   /note="HTH araC/xylS-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        178..199
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
FT   DNA_BIND        225..248
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00593"
SQ   SEQUENCE   262 AA;  30550 MW;  3CB18EA52DE53443 CRC64;
     MMDMGHKNKI DIKVRLHNYI ILYAKRCSMT VSSGNETLTI DEGQIAFIER NIQINVSIKK
     SDSINPFEII SLDRNLLLSI IRIMEPIYSF QHSYSEEKRG LNKKIFLLSE EEVSIDLFKS
     IKEMPFGKRK IYSLACLLSA VSDEEALYTS ISIASSLSFS DQIRKIVEKN IEKRWRLSDI
     SNNLNLSEIA VRKRLESEKL TFQQILLDIR MHHAAKLLLN SQSYINDVSR LIGISSPSYF
     IRKFNEYYGI TPKKFYLYHK KF