VIRG_AGRFC
ID VIRG_AGRFC Reviewed; 253 AA.
AC P07545;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Regulatory protein VirG;
GN Name=virG; OrderedLocusNames=Atu6178; ORFNames=AGR_pTi_15;
OS Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens
OS (strain C58)).
OG Plasmid pTiC58.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC Agrobacterium tumefaciens complex.
OX NCBI_TaxID=176299;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3448462; DOI=10.1111/j.1365-2958.1987.tb01937.x;
RA Powell B.S., Powell G.K., Morris R.O., Rogowsky P.M., Kado C.I.;
RT "Nucleotide sequence of the virG locus of the Agrobacterium tumefaciens
RT plasmid pTiC58.";
RL Mol. Microbiol. 1:309-316(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2194232; DOI=10.1016/0147-619x(90)90028-b;
RA Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P., Zyprian E.M.,
RA Steck T.R., Kado C.I.;
RT "Molecular characterization of the vir regulon of Agrobacterium
RT tumefaciens: complete nucleotide sequence and gene organization of the
RT 28.63-kbp regulon cloned as a single unit.";
RL Plasmid 23:85-106(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=D10B/87;
RX PubMed=7601840; DOI=10.1128/jb.177.13.3752-3757.1995;
RA Belanger C., Canfield M.L., Moore L.W., Dion P.;
RT "Genetic analysis of nonpathogenic Agrobacterium tumefaciens mutants
RT arising in crown gall tumors.";
RL J. Bacteriol. 177:3752-3757(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11743193; DOI=10.1126/science.1066804;
RA Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K.,
RA Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y.,
RA Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P.,
RA Clendenning J., Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R.,
RA Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G.,
RA Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y.,
RA Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S.,
RA Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F.,
RA Gordon M.P., Olson M.V., Nester E.W.;
RT "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT C58.";
RL Science 294:2317-2323(2001).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C58 / ATCC 33970;
RX PubMed=11743194; DOI=10.1126/science.1066803;
RA Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B.,
RA Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K.,
RA Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C., Allinger M.,
RA Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C.,
RA Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S.;
RT "Genome sequence of the plant pathogen and biotechnology agent
RT Agrobacterium tumefaciens C58.";
RL Science 294:2323-2328(2001).
CC -!- FUNCTION: VirG is required for the positive regulation of at least two
CC vir loci encoded by the Ti plasmid of A.tumefaciens.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- PTM: Phosphorylated by wide host range (WHR) VirA protein.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA86981.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAA91602.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M36786; AAD15211.1; -; Genomic_DNA.
DR EMBL; Y00535; CAA68595.1; -; Genomic_DNA.
DR EMBL; J03320; AAA91602.1; ALT_INIT; Genomic_DNA.
DR EMBL; U16142; AAA86981.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE007871; AAK90940.1; -; Genomic_DNA.
DR PIR; AH3249; AH3249.
DR PIR; S03760; S03760.
DR RefSeq; NP_396499.1; NC_003065.3.
DR RefSeq; WP_010891492.1; NC_003065.3.
DR AlphaFoldDB; P07545; -.
DR SMR; P07545; -.
DR IntAct; P07545; 2.
DR EnsemblBacteria; AAK90940; AAK90940; Atu6178.
DR GeneID; 39477123; -.
DR KEGG; atu:Atu6178; -.
DR PATRIC; fig|176299.10.peg.5372; -.
DR HOGENOM; CLU_000445_30_4_5; -.
DR OMA; VWGWDFG; -.
DR PhylomeDB; P07545; -.
DR BioCyc; AGRO:ATU6178-MON; -.
DR Proteomes; UP000000813; Plasmid Ti.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000156; F:phosphorelay response regulator activity; TAS:PAMGO_GAT.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd00383; trans_reg_C; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR011006; CheY-like_superfamily.
DR InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR InterPro; IPR039420; WalR-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR48111; PTHR48111; 1.
DR Pfam; PF00072; Response_reg; 1.
DR Pfam; PF00486; Trans_reg_C; 1.
DR SMART; SM00448; REC; 1.
DR SMART; SM00862; Trans_reg_C; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF52172; SSF52172; 1.
DR PROSITE; PS51755; OMPR_PHOB; 1.
DR PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE 3: Inferred from homology;
KW Activator; Crown gall tumor; Cytoplasm; DNA-binding; Phosphoprotein;
KW Plasmid; Reference proteome; Transcription; Transcription regulation;
KW Two-component regulatory system.
FT CHAIN 1..253
FT /note="Regulatory protein VirG"
FT /id="PRO_0000081265"
FT DOMAIN 15..129
FT /note="Response regulatory"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT DNA_BIND 141..241
FT /note="OmpR/PhoB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT MOD_RES 64
FT /note="4-aspartylphosphate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ SEQUENCE 253 AA; 28416 MW; 2BCFF0DDEC7217C4 CRC64;
MAGQDPRLRG EPLKHVLVID DDVAMRHLIV EYLTIHAFKV TAVADSKQFN RVLCSETVDV
VVVDLNLGRE DGLEIVRSLA TKSDVPIIII SGARLEEADK VIALELGATD FIAKPFGTRE
FLARIRVALR VRPSVARTKD RRSFSFADWT LNLRRRRLIS EEGSEVKLTA GEFNLLVAFL
EKPRDVLSRE QLLIASRVRE EEVYDRSIDV LILRLRRKLE GDPTTPQLIK TARGAGYFFD
ADVDVSYGGV MAA
