ID   VIRG_AGRT9              Reviewed;         267 AA.
AC   P62722; P06664;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Regulatory protein VirG;
GN   Name=virG;
OS   Agrobacterium tumefaciens (strain 15955).
OG   Plasmid pTi15955.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=190386;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3027669; DOI=10.1093/nar/14.24.9933;
RA   Melchers L.S., Thompson D.V., Idler K.B., Schilperoort R.A.,
RA   Hooykaas P.J.J.;
RT   "Nucleotide sequence of the virulence gene virG of the Agrobacterium
RT   tumefaciens octopine Ti plasmid: significant homology between virG and the
RT   regulatory genes ompR, phoB and dye of E. coli.";
RL   Nucleic Acids Res. 14:9933-9942(1986).
CC   -!- FUNCTION: VirG is required for the positive regulation of at least two
CC       vir loci encoded by the Ti plasmid of A.tumefaciens.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- PTM: Phosphorylated by wide host range (WHR) VirA protein.
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DR   EMBL; X04965; CAA28638.1; -; Genomic_DNA.
DR   PIR; A25519; A25519.
DR   AlphaFoldDB; P62722; -.
DR   SMR; P62722; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Crown gall tumor; Cytoplasm; DNA-binding; Phosphoprotein;
KW   Plasmid; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..267
FT                   /note="Regulatory protein VirG"
FT                   /id="PRO_0000081267"
FT   DOMAIN          29..143
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        155..255
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         78
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   267 AA;  29956 MW;  39D7239989435667 CRC64;
     MIVHPSRENF SSAVNKGSDF RLKGEPLKHV LLVDDDVAMR HLIIEYLTIH AFKVTAVADS
     TQFTRVLSSA TVDVVVVDLN LGREDGLEIV RNLAAKSDIP IIIISGDRLE ETDKVVALEL
     GASDFIAKPF SIREFLARIR VALRVRPNVV RSKDRRSFCF TDWTLNLRQR RLMSEAGGEV
     KLTAGEFNLL LAFLEKPRDV LSREQLLIAS RVRDEEVYDR SIDVLILRLR RKLEADPSSP
     QLIKTARGAG YFFDADVQVS HGGTMAA