ID   VIRG_RHIRD              Reviewed;         267 AA.
AC   Q44444; P06664;
DT   01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1988, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Regulatory protein VirG;
GN   Name=virG;
OS   Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium
OS   radiobacter).
OG   Plasmid pTiA6, and Plasmid pTiBo542.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=358;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pTiA6;
RX   PubMed=3022288; DOI=10.1073/pnas.83.21.8278;
RA   Winans S.C., Ebert P.R., Stachel S.E., Gordon M.P., Nester E.W.;
RT   "A gene essential for Agrobacterium virulence is homologous to a family of
RT   positive regulatory loci.";
RL   Proc. Natl. Acad. Sci. U.S.A. 83:8278-8282(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   PLASMID=pTiBo542;
RX   PubMed=1745238; DOI=10.1007/bf00290681;
RA   Chen C.Y., Wang L., Winans S.C.;
RT   "Characterization of the supervirulent virG gene of the Agrobacterium
RT   tumefaciens plasmid pTiBo542.";
RL   Mol. Gen. Genet. 230:302-309(1991).
CC   -!- FUNCTION: VirG is required for the positive regulation of at least two
CC       vir loci encoded by the Ti plasmid of A.tumefaciens.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- PTM: Phosphorylated by wide host range (WHR) VirA protein.
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DR   EMBL; J03216; AAA88657.1; -; Genomic_DNA.
DR   EMBL; X62885; CAA44677.1; -; Genomic_DNA.
DR   PIR; A25519; A25519.
DR   RefSeq; WP_012478082.1; NZ_LXKY01000002.1.
DR   RefSeq; YP_001967542.1; NC_010929.1.
DR   AlphaFoldDB; Q44444; -.
DR   SMR; Q44444; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF46894; SSF46894; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Crown gall tumor; Cytoplasm; DNA-binding; Phosphoprotein;
KW   Plasmid; Transcription; Transcription regulation;
KW   Two-component regulatory system.
FT   CHAIN           1..267
FT                   /note="Regulatory protein VirG"
FT                   /id="PRO_0000081266"
FT   DOMAIN          29..143
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        155..255
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         78
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   VARIANT         33
FT                   /note="V -> I (in plasmid pTiBo542)"
FT   VARIANT         82
FT                   /note="V -> G (in plasmid pTiBo542)"
FT   VARIANT         132
FT                   /note="I -> T (in plasmid pTiBo542)"
SQ   SEQUENCE   267 AA;  29998 MW;  236C829223F8F767 CRC64;
     MIVHPSRENF SSAVNKGSDF RLKGEPLKHV LLVDDDVAMR HLIIEYLTIH AFKVTAVADS
     TQFTRVLSSA TVDVVVVDLN LVREDGLEIV RNLAAKSDIP IIIISGDRLE ETDKVVALEL
     GASDFIAKPF SIREFLARIR VALRVRPNVV RSKDRRSFCF TDWTLNLRQR RLMSEAGGEV
     KLTAGEFNLL LAFLEKPRDV LSREQLLIAS RVRDEEVYDR SIDVLILRLR RKLEADPSSP
     QLIKTARGAG YFFDADVQVS HGGTMAA