VIR_ARATH
ID VIR_ARATH Reviewed; 2138 AA.
AC F4J8G7; F4J8G6; Q9M9X3;
DT 10-OCT-2018, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 70.
DE RecName: Full=Protein virilizer homolog {ECO:0000305};
DE AltName: Full=Protein EMBRYO DEFECTIVE 2016 {ECO:0000303|PubMed:15266054};
GN Name=VIR {ECO:0000303|PubMed:28503769};
GN Synonyms=EMB2016 {ECO:0000303|PubMed:15266054};
GN OrderedLocusNames=At3g05680 {ECO:0000312|Araport:AT3G05680};
GN ORFNames=F18C1.5 {ECO:0000312|EMBL:AAF26129.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP DISRUPTION PHENOTYPE [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Columbia;
RX PubMed=15266054; DOI=10.1104/pp.104.045179;
RA Tzafrir I., Pena-Muralla R., Dickerman A., Berg M., Rogers R., Hutchens S.,
RA Sweeney T.C., McElver J., Aux G., Patton D., Meinke D.;
RT "Identification of genes required for embryo development in Arabidopsis.";
RL Plant Physiol. 135:1206-1220(2004).
RN [4]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH MTB; FIP37
RP AND HAKAI, AND SUBCELLULAR LOCATION.
RX PubMed=28503769; DOI=10.1111/nph.14586;
RA Ruzicka K., Zhang M., Campilho A., Bodi Z., Kashif M., Saleh M.,
RA Eeckhout D., El-Showk S., Li H., Zhong S., De Jaeger G., Mongan N.P.,
RA Hejatko J., Helariutta Y., Fray R.G.;
RT "A mRNA methylation in Arabidopsis reveals a role for the conserved E3
RT ubiquitin ligase HAKAI.";
RL New Phytol. 215:157-172(2017).
CC -!- FUNCTION: Subunit of the N6-methyltransferase complex, a multiprotein
CC complex that mediates N6-methyladenosine (m6A) methylation at the 5'-
CC [AG]GAC-3' consensus sites of some mRNAs (PubMed:28503769). Associates
CC with MTA, MTB, FIP37 and HAKAI to form the m6A writer complex which is
CC essential for adenosine methylation at specific mRNA sequences
CC (PubMed:28503769). N6-methyladenosine (m6A) plays a role in mRNA
CC stability, processing, translation efficiency and editing
CC (PubMed:28503769). {ECO:0000269|PubMed:28503769}.
CC -!- SUBUNIT: Interacts with MTB, FIP37 and HAKAI (PubMed:28503769).
CC Associates with MTA, MTB, FIP37 and HAKAI to form the m6A writer
CC complex which is essential for adenosine methylation at specific mRNA
CC sequences (PubMed:28503769). {ECO:0000269|PubMed:28503769}.
CC -!- SUBCELLULAR LOCATION: Nucleus speckle {ECO:0000269|PubMed:28503769}.
CC Nucleus, nucleoplasm {ECO:0000269|PubMed:28503769}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality due to embryo development
CC arrest at globular stage. {ECO:0000269|PubMed:28503769}.
CC -!- MISCELLANEOUS: Plants silencing VIR exhibit pleiotropic phenotypes,
CC including aberrant root cap formation, gravity response and lateral
CC root development, and defective development of cotyledons.
CC {ECO:0000269|PubMed:28503769}.
CC -!- SIMILARITY: Belongs to the vir family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26129.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC011620; AAF26129.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE74277.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74278.2; -; Genomic_DNA.
DR RefSeq; NP_001319481.1; NM_001337599.1.
DR RefSeq; NP_187219.5; NM_111442.7.
DR AlphaFoldDB; F4J8G7; -.
DR STRING; 3702.AT3G05680.2; -.
DR iPTMnet; F4J8G7; -.
DR PRIDE; F4J8G7; -.
DR ProMEX; F4J8G7; -.
DR ProteomicsDB; 242319; -.
DR EnsemblPlants; AT3G05680.1; AT3G05680.1; AT3G05680.
DR EnsemblPlants; AT3G05680.2; AT3G05680.2; AT3G05680.
DR GeneID; 819736; -.
DR Gramene; AT3G05680.1; AT3G05680.1; AT3G05680.
DR Gramene; AT3G05680.2; AT3G05680.2; AT3G05680.
DR KEGG; ath:AT3G05680; -.
DR Araport; AT3G05680; -.
DR TAIR; locus:2078082; AT3G05680.
DR eggNOG; KOG4822; Eukaryota.
DR HOGENOM; CLU_001542_0_0_1; -.
DR OMA; EIFKVHQ; -.
DR OrthoDB; 20412at2759; -.
DR PRO; PR:F4J8G7; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4J8G7; baseline and differential.
DR GO; GO:0016607; C:nuclear speck; IDA:TAIR.
DR GO; GO:0005654; C:nucleoplasm; IDA:TAIR.
DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IBA:GO_Central.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IDA:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:0080009; P:mRNA methylation; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0009651; P:response to salt stress; IMP:TAIR.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR InterPro; IPR031801; VIR_N.
DR InterPro; IPR026736; Virilizer.
DR PANTHER; PTHR23185; PTHR23185; 2.
DR Pfam; PF15912; VIR_N; 1.
PE 1: Evidence at protein level;
KW mRNA processing; mRNA splicing; Nucleus; Reference proteome.
FT CHAIN 1..2138
FT /note="Protein virilizer homolog"
FT /id="PRO_0000445520"
FT REGION 1503..1574
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1638..1664
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1855..1881
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2013..2035
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2058..2094
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1522..1545
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1644..1663
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1857..1881
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2138 AA; 234225 MW; FB264E00F0701704 CRC64;
MVRSEPCVLF AQTFVHPQLD EYVDEVIFAE PVIITACEFL EQNASSSSQA VSLVGATSPP
SFALEVFVRC EGESKFKRLC NPFLYTPSAP YPLEVEAVVT NHLVVRGSYR SLSLIVYGNI
VKDLGQYNII LEGRSVTDIV SSTEGNLEDL PLVLHSVNRT IEECLSSLDI VSLPLAAVDL
PVEVKRLLQL LLKIFDKLAT NDVVNKFVDT VVSGVSSYVT DNVDFFLKNK NCSAVTSSLD
SGLFHDIVDR VKEDILDLNE IQESDVALGL FSFLESETYL ATSQQLVVML SPYIQFERDS
LCTVLPKLSK GKATLLGLSL AFLLCSGREG CLQFVNSGGM DQLVYLFGHD GQNSTTITLL
LLGVVEQATR HSVGCEGFLG WWPREDGSIP SGKSEGYCLL LKLLMQKPCH EIASLAIYIL
RRLRIYEVIS RYEFAVLSAL EGLSNSHGAA THNLNMLSDA KSQLQKLQNL MKSLGSVEDP
SPSAYAERSL VSDHSEGWLS YKATSKLTSS WTCPFYSSGI DSHILALLKE RGFLPLSAAL
LSMPELHSKV GDIMDVFTDI AMFIGNIILS FMFSRTGLSF LLHHPELTAT IIQSLKGSVD
LNKEECVPLH YASILISKGF TCSLLEIGIN LEMHLRVVSA VDRLLKSIQQ TEEFLWILWE
LRDVSRSDCG REALLTLGVF PEALAVLIEA LHSAKDMEPA VENSGISPLN LAICHSAAEI
FEVIVSDSTA SCLHAWIEHA PVLHKALHTL SPGGSNRKDA PSRLLKWIDA GVVYHKHGVG
GLLRYAAVLA SGGDAQLSSS SILALDLTPA ENGAGESTNV SEMNVLDNLG KVIFEKSFEG
VNLSDSSISQ LTTALRILAL ISDNSTVAAA LYDEGAVTVV YAILVNCSFM FERSSNIYDY
LVDDDHGCSS ISDFLSERNR EQSLVDLLIP SLVLLISVLQ RLQGTKEQYR NTKLMKALLR
LHREVSPKLA ACAADLSSHY PDSALGFGAV CHLIVSALVC WPVYGWIPGL FHTLLSGVQT
SSVPALGPKE TCSFLCILSD ILPEEGVWFW KSGMPLLSGL RKLAVGTLMG PQKEKQINWY
LEPGPLEKLI NHLTPNLDKI AKIIQHHAVS ALVVIQDMLR VFIVRIACQR VEHASILLRP
IFSSIRDGIL DQSSTRDTEA YMVYRYLNFL ASLLEHPHAK GLLLEEGIVQ LLVEVLERCY
DATYPSENRV LEYGIVSASS VIQWCIPAFR SISLLCDSQV PLLCFQKKEL LASLSAKDCA
LIFPFVLKFC QVLPVGNELL SCLGAFKDLS SCGEGQDGLV SLLFHLFSGT EESVSERWCD
TNSLSLDQLD MKKNPPFLSC WIKLLNSINS KDGLSSLAMK AVNVLSVGSI RLCLDGKSLD
SKKVAALKSL FGLPSEFSGT DTFREENIGL IEQMVTLLSS MTSGSDSSAT AEMKPYLHEA
SQSLLSLLKD GNIDDIISCK GVFVSPGNLD MDDLVSRNIE DDLYQRGLED KFWWECPETL
PERLPQSSLP AKRKLPTLES SSRRAKGENS SVDIPTQNSI QRGMGSVSLP PAPTRRDAFR
QRKPNTSRPP SMHVDDYVAR ERSVDTAGNS NAITISRAGS SSGRPPSIHV DEFMARQRER
GQNPSTIVVG EAVVQVKNPT PARDTEKVAG KPKQFKADPD DDLQGIDIVF DGEECEGPDD
KLPFLQPDEN LMQPAPVMVE QNSPHSIVEE TESDANGSSQ FSHMGTPVAS NVDENAQSEF
SSRISVSRPE MSLIREPSIS SDRKFVEQAD EAKKMAPLKS AGISESGFIP AYHMPGSSGQ
NSIDPRVGPQ GFYSKSGQQH TGHIHGGFSG RGVYEQKVMP NQPPLPLVPP PSVSPVIPHS
SDSLSNQSSP FISHGTQSSG GPTRLMPPLP SAIPQYSSNP YASLPPRTST VQSFGYNHAG
VGTTEQQQSG PTIDHQSGNL SVTGMTSYPP PNLMPSHNFS RPSSLPVPFY GNPSHQGGDK
PQTMLLVPSI PQSLNTQSIP QLPSMQLSQL QRPMQPPQHV RPPIQISQPS EQGVSMQNPF
QIPMHQMQLM QQTQVQPYYH PPQQQEISQV QQQQQHHAVQ GQQGAGTSQQ QESGMSLHDY
FKSPEAIQAL LSDRDKLCQL LEQHPKLMQM LQEKLGQL
