VIR_DROPS
ID VIR_DROPS Reviewed; 1849 AA.
AC Q291E4;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 66.
DE RecName: Full=Protein virilizer {ECO:0000305};
GN Name=vir; ORFNames=GA17481;
OS Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=46245;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MV2-25 / Tucson 14011-0121.94;
RX PubMed=15632085; DOI=10.1101/gr.3059305;
RA Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O.,
RA Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F.,
RA Howells S.L., Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A.,
RA Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M.,
RA Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J.,
RA Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y.,
RA Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA Weinstock G.M., Gibbs R.A.;
RT "Comparative genome sequencing of Drosophila pseudoobscura: chromosomal,
RT gene, and cis-element evolution.";
RL Genome Res. 15:1-18(2005).
CC -!- FUNCTION: Associated component of the WMM complex, a complex that
CC mediates N6-methyladenosine (m6A) methylation of mRNAs, a modification
CC that plays a role in the efficiency of mRNA splicing and is required
CC for sex determination. Required for sex determination and dosage
CC compensation via Sxl alternative splicing: m6A methylation acts as a
CC key regulator of Sxl pre-mRNA and promotes female-specific alternative
CC splicing of Sxl, which determines female physiognomy. M6A methylation
CC is also required for neuronal functions. Required for proper inclusion
CC of regulated exons in Ubx transcripts, leading to isoforms Ia/b and
CC IIa/b. {ECO:0000250|UniProtKB:Q9W1R5}.
CC -!- SUBUNIT: Component of the WMM complex, a N6-methyltransferase complex
CC composed of a catalytic subcomplex, named MAC, and of an associated
CC subcomplex, named MACOM. The MAC subcomplex is composed of Ime4/Mettl3
CC and Mettl14. The MACOM subcomplex is composed of fl(2)d, Flacc/Xio,
CC Hakai, vir, and, in some cases of nito. Part of a complex containing
CC fl(2)d, Sxl and vir. {ECO:0000250|UniProtKB:Q9W1R5}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9W1R5}.
CC -!- SIMILARITY: Belongs to the vir family. {ECO:0000305}.
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DR EMBL; CM000071; EAL25168.1; -; Genomic_DNA.
DR RefSeq; XP_001360593.1; XM_001360556.3.
DR AlphaFoldDB; Q291E4; -.
DR STRING; 7237.FBpp0276805; -.
DR EnsemblMetazoa; FBtr0278367; FBpp0276805; FBgn0077492.
DR GeneID; 4803955; -.
DR KEGG; dpo:Dpse_GA17481; -.
DR eggNOG; KOG4822; Eukaryota.
DR HOGENOM; CLU_002368_0_0_1; -.
DR InParanoid; Q291E4; -.
DR OMA; CERKQIP; -.
DR PhylomeDB; Q291E4; -.
DR Proteomes; UP000001819; Chromosome 3.
DR Bgee; FBgn0077492; Expressed in female reproductive system and 3 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0036396; C:RNA N6-methyladenosine methyltransferase complex; IEA:EnsemblMetazoa.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0080009; P:mRNA methylation; IEA:EnsemblMetazoa.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:1903688; P:positive regulation of border follicle cell migration; IEA:EnsemblMetazoa.
DR GO; GO:0007539; P:primary sex determination, soma; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000375; P:RNA splicing, via transesterification reactions; ISS:UniProtKB.
DR GO; GO:0007548; P:sex differentiation; IEA:UniProtKB-KW.
DR InterPro; IPR031801; VIR_N.
DR InterPro; IPR026736; Virilizer.
DR PANTHER; PTHR23185; PTHR23185; 1.
DR Pfam; PF15912; VIR_N; 1.
PE 3: Inferred from homology;
KW Developmental protein; Differentiation; mRNA processing; mRNA splicing;
KW Nucleus; Reference proteome; Sexual differentiation.
FT CHAIN 1..1849
FT /note="Protein virilizer"
FT /id="PRO_0000308608"
FT REGION 206..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 783..818
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1557..1584
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1666..1686
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1715..1782
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1798..1849
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..228
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..294
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..324
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 332..350
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 786..814
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1562..1584
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1798..1832
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1849 AA; 208677 MW; 544E18A1A82B72EB CRC64;
MTEVDDGAEL LFFDTFSHED ITDINLDLVQ FPKPVFITQV RIIPLGARVQ ADFPGGVRLG
ATNPSKFDLE FFVNDLGMPG ASAFENLGLL RYNQNDCIHL DCSREKIPTD GLVLRGWYST
ITLAVYGILT NSVTQPIASP TLPCEPAGPE MCNLSTEAHN VLLQEEVLKD DWAEPIPTEL
LVPHKAVVVS DYEHDDIEYG LSREHQHYHQ HVDEEQREMR RLRRSTHSTD RSPPPPPMRT
HTHSESNERE YIRCSRDKGS RDWSRSPEYS SHRSRRKRSD RSRSDADEHK WPRTPPASID
SPIRPRSPDA MDYDDDDSRS HYKMQASRGY RHSSESLHRD RDDEERSGTP QEQFEPILSD
DEIIGDDEED EAEDVAAAAE YERELEFAAA VAPPAIDAFE PWQRPLLVYE GDLAAHLNKE
LETLRLLFKK LSLQTRCESV TAFSDEHGLS ADEREQFVYL GEQLNNQLGY LSQHYKRRNF
VLQQFFSHDD AHLRQAANVL QVALSFQAAC MQPQPAFKIR HIKLGARMAE LLGSNEQLFG
HLLQEQDFDL FEAVLSLYKE PYMALSIKLQ LLKAVYAMLD TRLGVQNFLS PKTNGYQMVM
DFLKTAKLTR TKYALQAIIK KLHLYEALAS VQQCCRQIFL DTDASVPETT PDPNRLDVCQ
KIEFAFQMLM DALTDSQLSY QQPRRFLPVS KKFEVLTDPT AQRSFGNALQ SYFVQHSLAE
ALLVILSNPK EVPASTFLCT LDLMHTLLRS HVGIDYFVDD AFPVTQMIVS ILLGLEEVPS
QPRVVEAKPE GKEDKPMDDS VEQKPDEGKA AGIRAAEEPK MAPPPVVRKP ILRPVLPRLA
RLGIELSYKV QTRYHLDAIT FTAACPEYDT IKIATHMHSI YSQTCDPSGR QHTIEVLGLN
NNLKIFMDLI KKEQRLQTQR QLLSSPGTKY KSPVLSYAVD MVDACVRYCE QLDYLIEHGG
VILELAKNHE TFEPSVSAVL QEMYVYMKPL EAINVFVYDD IMPLVEVIGR SLDYLTTFPG
DLIMALRILR FLAISKPRQK SHGTEELKHR FVALQLYAAD GVQLLLQIME RLCTHFEQPG
IHAPALMTIQ GVHCCQIMLP TLQILRELLS YAILCRDGTY KDLTAIDHLV KVYYLLYYYP
SRCQAGAEVE QCKLEVVKSL LAYTQPHEQD AESLHKSLWT LMVREVLKNI DGPAHFIPGL
KLLAELLPLP LPMPQPLCDQ LKQQHKQRLI IERKLWSAHL HPQSGQIAKL VEALAPSSFP
QLAELLQRVC MQLSDLAPNM TLLIAKTITE LLCTEYQTSN CIPTTNLERL LRFSTRLCAF
APLKSSMLSI LSGKFWELFQ SLLALNEFNE VVTNCQEAVH RILDSFLDSG ISLISHKSTA
QPALNLSAAL PPKELIPRII DAVFSNLTSV EVTHGISILA VRNLVILTEH DFTFYHLAQL
LKQKLTEFQA WMERVILHNE TVEYHANIES LILLLRSLTQ IEPPPPMCAM PNRTLKLGAM
ELAQLVEFQD IDMARPPVLA RILRVLEKYK LLANEAALCD LKQLIVLHAS RQEAMGSASM
ETPAVETEND GANPAASCST SSSGGSLNVE PFLPQAEGIV TQYDARPIFT RYCATAENPQ
LTARYWLEPL PIELIEDMNE PLYERIACDL TDLANVCLNP DLATSGESKR TLNLSGSPQS
NREMTPTAPC FRTRRVEVEP ATGRPEKKMF LSSVRGRGFA RPPPSRGDLF RSRPPNTSRP
PSLHVDDFLA LETCGAQPTG PTGYNKIPSM LRGSRVGRNR GSRISAAAAF RQKKMMRIGS
PSSWTESGGG SYRSTSESHF GGSDSHYSSP HYSGRSRGRG LRSRPPYLR
