VITRN_HUMAN
ID VITRN_HUMAN Reviewed; 678 AA.
AC Q6UXI7; A1A526; A6NKI9; A8K7Y4; E9PF47; Q6P7T3; Q96DM8; Q96DT1; Q9UDN0;
DT 05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Vitrin;
DE Flags: Precursor;
GN Name=VIT; ORFNames=UNQ647/PRO1277;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Skeletal muscle;
RX PubMed=10830112; DOI=10.1042/bst0270832;
RA Mayne R., Ren Z.-X., Liu J.G., Cook T., Carson M., Narayana S.;
RT "VIT-1: the second member of a new branch of the von Willebrand factor A
RT domain superfamily.";
RL Biochem. Soc. Trans. 27:832-835(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 4).
RC TISSUE=Placenta, and Synovium;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 3 AND 5), AND VARIANTS
RP ARG-555; ILE-559 AND TYR-658.
RC TISSUE=PNS;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Promotes matrix assembly and cell adhesiveness. Plays a role
CC in spinal cord formation by regulating the proliferation and
CC differentiation of neural stem cells. {ECO:0000250|UniProtKB:Q8VHI5}.
CC -!- SUBUNIT: Binds dermatan sulfate and chondroitin sulfate. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=1;
CC IsoId=Q6UXI7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6UXI7-2; Sequence=VSP_020211;
CC Name=3;
CC IsoId=Q6UXI7-3; Sequence=VSP_020209, VSP_020210;
CC Name=4;
CC IsoId=Q6UXI7-4; Sequence=VSP_040124;
CC Name=5;
CC IsoId=Q6UXI7-5; Sequence=VSP_044597;
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DR EMBL; AF063833; AAL18263.1; -; mRNA.
DR EMBL; AY358338; AAQ88704.1; -; mRNA.
DR EMBL; AK056772; BAB71279.1; -; mRNA.
DR EMBL; AK292149; BAF84838.1; -; mRNA.
DR EMBL; AC007363; AAF19243.2; -; Genomic_DNA.
DR EMBL; CH471053; EAX00416.1; -; Genomic_DNA.
DR EMBL; BC061519; AAH61519.1; -; mRNA.
DR EMBL; BC128260; AAI28261.1; -; mRNA.
DR CCDS; CCDS33180.1; -. [Q6UXI7-4]
DR CCDS; CCDS54347.1; -. [Q6UXI7-1]
DR CCDS; CCDS54348.1; -. [Q6UXI7-5]
DR CCDS; CCDS54349.1; -. [Q6UXI7-2]
DR CCDS; CCDS54350.1; -. [Q6UXI7-3]
DR RefSeq; NP_001171440.1; NM_001177969.1. [Q6UXI7-1]
DR RefSeq; NP_001171441.1; NM_001177970.1. [Q6UXI7-5]
DR RefSeq; NP_001171442.1; NM_001177971.1. [Q6UXI7-2]
DR RefSeq; NP_001171443.1; NM_001177972.1. [Q6UXI7-3]
DR RefSeq; NP_444506.2; NM_053276.3. [Q6UXI7-4]
DR AlphaFoldDB; Q6UXI7; -.
DR SMR; Q6UXI7; -.
DR BioGRID; 111233; 2.
DR IntAct; Q6UXI7; 2.
DR STRING; 9606.ENSP00000368544; -.
DR GlyGen; Q6UXI7; 3 sites, 1 O-linked glycan (1 site).
DR iPTMnet; Q6UXI7; -.
DR PhosphoSitePlus; Q6UXI7; -.
DR BioMuta; VIT; -.
DR DMDM; 74739159; -.
DR EPD; Q6UXI7; -.
DR jPOST; Q6UXI7; -.
DR MassIVE; Q6UXI7; -.
DR PaxDb; Q6UXI7; -.
DR PeptideAtlas; Q6UXI7; -.
DR PRIDE; Q6UXI7; -.
DR ProteomicsDB; 20025; -.
DR ProteomicsDB; 67627; -. [Q6UXI7-1]
DR ProteomicsDB; 67628; -. [Q6UXI7-2]
DR ProteomicsDB; 67629; -. [Q6UXI7-3]
DR ProteomicsDB; 67630; -. [Q6UXI7-4]
DR Antibodypedia; 29304; 59 antibodies from 15 providers.
DR DNASU; 5212; -.
DR Ensembl; ENST00000379241.7; ENSP00000368543.3; ENSG00000205221.13. [Q6UXI7-2]
DR Ensembl; ENST00000379242.8; ENSP00000368544.3; ENSG00000205221.13. [Q6UXI7-4]
DR Ensembl; ENST00000389975.7; ENSP00000374625.3; ENSG00000205221.13. [Q6UXI7-1]
DR Ensembl; ENST00000401530.5; ENSP00000385658.1; ENSG00000205221.13. [Q6UXI7-5]
DR Ensembl; ENST00000457137.6; ENSP00000393561.2; ENSG00000205221.13. [Q6UXI7-3]
DR GeneID; 5212; -.
DR KEGG; hsa:5212; -.
DR MANE-Select; ENST00000379242.8; ENSP00000368544.3; NM_053276.4; NP_444506.2. [Q6UXI7-4]
DR UCSC; uc002rpk.4; human. [Q6UXI7-1]
DR CTD; 5212; -.
DR DisGeNET; 5212; -.
DR GeneCards; VIT; -.
DR HGNC; HGNC:12697; VIT.
DR HPA; ENSG00000205221; Tissue enhanced (tongue).
DR MIM; 617693; gene.
DR neXtProt; NX_Q6UXI7; -.
DR OpenTargets; ENSG00000205221; -.
DR PharmGKB; PA37316; -.
DR VEuPathDB; HostDB:ENSG00000205221; -.
DR eggNOG; KOG1216; Eukaryota.
DR GeneTree; ENSGT00940000159330; -.
DR HOGENOM; CLU_116785_0_0_1; -.
DR InParanoid; Q6UXI7; -.
DR OMA; KTCFNSA; -.
DR OrthoDB; 200139at2759; -.
DR PhylomeDB; Q6UXI7; -.
DR TreeFam; TF318242; -.
DR PathwayCommons; Q6UXI7; -.
DR SignaLink; Q6UXI7; -.
DR BioGRID-ORCS; 5212; 7 hits in 1062 CRISPR screens.
DR ChiTaRS; VIT; human.
DR GenomeRNAi; 5212; -.
DR Pharos; Q6UXI7; Tbio.
DR PRO; PR:Q6UXI7; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q6UXI7; protein.
DR Bgee; ENSG00000205221; Expressed in tibia and 128 other tissues.
DR ExpressionAtlas; Q6UXI7; baseline and differential.
DR Genevisible; Q6UXI7; HS.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; IBA:GO_Central.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0005614; C:interstitial matrix; IBA:GO_Central.
DR GO; GO:0005539; F:glycosaminoglycan binding; IEA:Ensembl.
DR GO; GO:0030198; P:extracellular matrix organization; IBA:GO_Central.
DR GO; GO:0010811; P:positive regulation of cell-substrate adhesion; IBA:GO_Central.
DR GO; GO:0021510; P:spinal cord development; ISS:UniProtKB.
DR Gene3D; 2.170.130.20; -; 1.
DR Gene3D; 3.40.50.410; -; 2.
DR InterPro; IPR004043; LCCL.
DR InterPro; IPR036609; LCCL_sf.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR Pfam; PF03815; LCCL; 1.
DR Pfam; PF00092; VWA; 2.
DR SMART; SM00603; LCCL; 1.
DR SMART; SM00327; VWA; 2.
DR SUPFAM; SSF53300; SSF53300; 2.
DR SUPFAM; SSF69848; SSF69848; 1.
DR PROSITE; PS50820; LCCL; 1.
DR PROSITE; PS50234; VWFA; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Extracellular matrix; Glycoprotein;
KW Neurogenesis; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..678
FT /note="Vitrin"
FT /id="PRO_0000248210"
FT DOMAIN 40..133
FT /note="LCCL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DOMAIN 293..478
FT /note="VWFA 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT DOMAIN 495..668
FT /note="VWFA 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00219"
FT REGION 154..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 199..244
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 390
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 520
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..62
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT DISULFID 66..86
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00123"
FT VAR_SEQ 164..203
FT /note="ETTKAYQRPPIPGTTAQPVTLMQLLAVTVAVATPTTLPRP -> KCSRVIES
FT KPSESMNTRRVLGDSGEINILTGQAPLALAIF (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_020209"
FT VAR_SEQ 204..678
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039,
FT ECO:0000303|PubMed:15489334"
FT /id="VSP_020210"
FT VAR_SEQ 247..268
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12975309"
FT /id="VSP_020211"
FT VAR_SEQ 267
FT /note="L -> LGEMDSWKPGSVLLDE (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_040124"
FT VAR_SEQ 268..289
FT /note="GLVPKEELSTQSLEPVSLGDPN -> D (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_044597"
FT VARIANT 555
FT /note="K -> R (in dbSNP:rs2072526)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_068964"
FT VARIANT 559
FT /note="L -> I (in dbSNP:rs2072525)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_068965"
FT VARIANT 658
FT /note="H -> Y (in dbSNP:rs11901202)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_068966"
FT CONFLICT 80
FT /note="A -> T (in Ref. 1; AAL18263)"
FT /evidence="ECO:0000305"
FT CONFLICT 93
FT /note="G -> S (in Ref. 1; AAL18263)"
FT /evidence="ECO:0000305"
FT CONFLICT 97
FT /note="N -> D (in Ref. 1; AAL18263)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="A -> T (in Ref. 1; AAL18263)"
FT /evidence="ECO:0000305"
FT CONFLICT 419
FT /note="E -> V (in Ref. 1; AAL18263)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 678 AA; 73930 MW; 9870E75A218C686C CRC64;
MRTVVLTMKA SVIEMFLVLL VTGVHSNKET AKKIKRPKFT VPQINCDVKA GKIIDPEFIV
KCPAGCQDPK YHVYGTDVYA SYSSVCGAAV HSGVLDNSGG KILVRKVAGQ SGYKGSYSNG
VQSLSLPRWR ESFIVLESKP KKGVTYPSAL TYSSSKSPAA QAGETTKAYQ RPPIPGTTAQ
PVTLMQLLAV TVAVATPTTL PRPSPSAAST TSIPRPQSVG HRSQEMDLWS TATYTSSQNR
PRADPGIQRQ DPSGAAFQKP VGADVSLGLV PKEELSTQSL EPVSLGDPNC KIDLSFLIDG
STSIGKRRFR IQKQLLADVA QALDIGPAGP LMGVVQYGDN PATHFNLKTH TNSRDLKTAI
EKITQRGGLS NVGRAISFVT KNFFSKANGN RSGAPNVVVV MVDGWPTDKV EEASRLARES
GINIFFITIE GAAENEKQYV VEPNFANKAV CRTNGFYSLH VQSWFGLHKT LQPLVKRVCD
TDRLACSKTC LNSADIGFVI DGSSSVGTGN FRTVLQFVTN LTKEFEISDT DTRIGAVQYT
YEQRLEFGFD KYSSKPDILN AIKRVGYWSG GTSTGAAINF ALEQLFKKSK PNKRKLMILI
TDGRSYDDVR IPAMAAHLKG VITYAIGVAW AAQEELEVIA THPARDHSFF VDEFDNLHQY
VPRIIQNICT EFNSQPRN
