VJBR_BRUA2
ID VJBR_BRUA2 Reviewed; 259 AA.
AC Q2YJ50;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=HTH-type quorum sensing-dependent transcriptional regulator VjbR {ECO:0000305};
GN Name=vjbR; OrderedLocusNames=BAB2_0118;
OS Brucella abortus (strain 2308).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=359391;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2308;
RX PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL Infect. Immun. 73:8353-8361(2005).
RN [2]
RP ABSENCE OF CLUMPING PHENOTYPE.
RX PubMed=17557825; DOI=10.1128/jb.00265-07;
RA Uzureau S., Godefroid M., Deschamps C., Lemaire J., De Bolle X.,
RA Letesson J.-J.;
RT "Mutations of the quorum sensing-dependent regulator VjbR lead to drastic
RT surface modifications in Brucella melitensis.";
RL J. Bacteriol. 189:6035-6047(2007).
RN [3]
RP INDUCTION.
RC STRAIN=2308;
RX PubMed=22530016; DOI=10.1371/journal.pone.0035394;
RA Arocena G.M., Zorreguieta A., Sieira R.;
RT "Expression of VjbR under nutrient limitation conditions is regulated at
RT the post-transcriptional level by specific acidic pH values and urocanic
RT acid.";
RL PLoS ONE 7:E35394-E35394(2012).
RN [4]
RP FUNCTION, AND DNA-BINDING.
RC STRAIN=2308;
RX PubMed=28334833; DOI=10.1093/nar/gkx165;
RA Kleinman C.L., Sycz G., Bonomi H.R., Rodriguez R.M., Zorreguieta A.,
RA Sieira R.;
RT "ChIP-seq analysis of the LuxR-type regulator VjbR reveals novel insights
RT into the Brucella virulence gene expression network.";
RL Nucleic Acids Res. 45:5757-5769(2017).
CC -!- FUNCTION: Transcriptional regulator involved in the global control of
CC Brucella gene expression (PubMed:28334833). Mediates the effects of the
CC quorum sensing autoinducer C12-HSL (N-dodecanoyl-homoserine lactone) on
CC a large and diverse number of genes (By similarity). Binds with high
CC specificity to a large number of sites distributed across the genome.
CC The VjbR-binding motif contains an asymmetric 16-bp consensus sequence,
CC an unusual feature among LuxR-type regulators (PubMed:28334833). May
CC directly modulate expression of many genes, including virulence
CC determinants, components of the respiratory chain, efflux systems,
CC elements predicted to participate in ion transport and adaptation to
CC stress, uncharacterized proteins, as well as transcriptional regulators
CC probably responsible for the observed indirect VjbR-mediated effects
CC (PubMed:28334833). {ECO:0000250|UniProtKB:Q8YAY5,
CC ECO:0000269|PubMed:28334833}.
CC -!- INDUCTION: Regulated at the post-transcriptional level. In rich medium,
CC protein is expressed only in the stationary phase of growth. In minimal
CC medium, protein is expressed only at pH 5.5 in the presence of urocanic
CC acid. {ECO:0000269|PubMed:22530016}.
CC -!- MISCELLANEOUS: A B.abortus vjbR deletion mutant complemented with the
CC defective B.melitensis D82A mutant does not display the typical
CC clumping phenotype observed in B.melitensis.
CC {ECO:0000269|PubMed:17557825}.
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DR EMBL; AM040265; CAJ12284.1; -; Genomic_DNA.
DR RefSeq; WP_002966460.1; NZ_KN046823.1.
DR AlphaFoldDB; Q2YJ50; -.
DR SMR; Q2YJ50; -.
DR STRING; 359391.BAB2_0118; -.
DR EnsemblBacteria; CAJ12284; CAJ12284; BAB2_0118.
DR GeneID; 45053211; -.
DR GeneID; 55591850; -.
DR KEGG; bmf:BAB2_0118; -.
DR PATRIC; fig|359391.11.peg.2065; -.
DR HOGENOM; CLU_072786_7_0_5; -.
DR OMA; RESLQWV; -.
DR PhylomeDB; Q2YJ50; -.
DR PHI-base; PHI:7607; -.
DR Proteomes; UP000002719; Chromosome II.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.450.80; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03472; Autoind_bind; 1.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF75516; SSF75516; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Quorum sensing; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..259
FT /note="HTH-type quorum sensing-dependent transcriptional
FT regulator VjbR"
FT /id="PRO_0000319926"
FT DOMAIN 183..248
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 207..226
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT REGION 76..179
FT /note="C12-HSL binding"
FT /evidence="ECO:0000255"
SQ SEQUENCE 259 AA; 28625 MW; 2D845BA0737094CC CRC64;
MSLDLVHFPN YKKTFFGSSF QSDTLALLTR IRDEIGCRYV THTYRGRVGD CTKVNSADLT
VLMTLPATWV ARYSSKNYFA IDPVFQEDAP YYRNDTSAIA RDLKEDADIC PAVAELLHDA
EKHGLGNLFI AVSARNPKGV AGCTVFTFEV EDEDRTQFLA RMRPRLLSLA GIIHGTVCGC
KDANSVASLL TPREVDCLRW AANGKTDGEI AEILSIARWT VVTYLQNAKI KLNCSNRTSA
VATALSLGII DMPEVQHLV
