VJBR_BRUME
ID VJBR_BRUME Reviewed; 259 AA.
AC Q8YAY5;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2008, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=HTH-type quorum sensing-dependent transcriptional regulator VjbR {ECO:0000305};
GN Name=vjbR; OrderedLocusNames=BMEII1116;
OS Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=224914;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=11756688; DOI=10.1073/pnas.221575398;
RA DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA Haselkorn R., Kyrpides N.C., Overbeek R.;
RT "The genome sequence of the facultative intracellular pathogen Brucella
RT melitensis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
RN [2]
RP FUNCTION IN REGULATION OF VIRB OPERON AND FLAGELLAR GENES, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=16008582; DOI=10.1111/j.1462-5822.2005.00543.x;
RA Delrue R.-M., Deschamps C., Leonard S., Nijskens C., Danese I.,
RA Schaus J.-M., Bonnot S., Ferooz J., Tibor A., De Bolle X., Letesson J.-J.;
RT "A quorum-sensing regulator controls expression of both the type IV
RT secretion system and the flagellar apparatus of Brucella melitensis.";
RL Cell. Microbiol. 7:1151-1161(2005).
RN [3]
RP FUNCTION IN REGULATION OF FLAGELLAR GENES, AND INTERACTION WITH FTCR.
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=17056750; DOI=10.1128/jb.00712-06;
RA Leonard S., Ferooz J., Haine V., Danese I., Fretin D., Tibor A.,
RA de Walque S., De Bolle X., Letesson J.-J.;
RT "FtcR is a new master regulator of the flagellar system of Brucella
RT melitensis 16M with homologs in Rhizobiaceae.";
RL J. Bacteriol. 189:131-141(2007).
RN [4]
RP FUNCTION IN REGULATION OF MEMBRANE STRUCTURES AND INTERNAL REPLICATION, AND
RP MUTAGENESIS OF ASP-82.
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=17557825; DOI=10.1128/jb.00265-07;
RA Uzureau S., Godefroid M., Deschamps C., Lemaire J., De Bolle X.,
RA Letesson J.-J.;
RT "Mutations of the quorum sensing-dependent regulator VjbR lead to drastic
RT surface modifications in Brucella melitensis.";
RL J. Bacteriol. 189:6035-6047(2007).
RN [5]
RP FUNCTION.
RC STRAIN=16M / ATCC 23456 / NCTC 10094;
RX PubMed=20529360; DOI=10.1186/1471-2180-10-167;
RA Weeks J.N., Galindo C.L., Drake K.L., Adams G.L., Garner H.R., Ficht T.A.;
RT "Brucella melitensis VjbR and C12-HSL regulons: contributions of the N-
RT dodecanoyl homoserine lactone signaling molecule and LuxR homologue VjbR to
RT gene expression.";
RL BMC Microbiol. 10:167-167(2010).
CC -!- FUNCTION: Transcriptional regulator involved in the global control of
CC Brucella gene expression. Mediates the effects of the quorum sensing
CC autoinducer C12-HSL (N-dodecanoyl-homoserine lactone) on a large and
CC diverse number of genes, including the virB operon and the flagellar
CC genes fliF and flgE, which are activated in the absence of C12-HSL.
CC Regulates virulence factors as well as several membrane structures.
CC Implicated in the control of outer membrane composition. Plays a role
CC in the expression of genes involved in the EPS synthesis and/or export.
CC Mediates the inhibitory effect of C12-HSL on B.melitensis intracellular
CC replication. Could activate expression of ftcR.
CC {ECO:0000269|PubMed:16008582, ECO:0000269|PubMed:17056750,
CC ECO:0000269|PubMed:17557825, ECO:0000269|PubMed:20529360}.
CC -!- DISRUPTION PHENOTYPE: Cells show down-regulated expression of both virB
CC operon and flagellar genes either during culture in bacteriological
CC medium or during intracellular infection. They also show no flgE or
CC fliC production between the end of the latent phase of growth and the
CC beginning of the exponential phase. The vjbR mutant is also strongly
CC attenuated in a mouse model of infection, probably because it is
CC defective in intracellular survival. When cultures reach high density,
CC cells aggregate and form clumps. The mutant is able to produce EPS
CC containing alpha-mannopyranosyl and/or alpha-glucopyranosyl residues as
CC well as a beta-linked glucan that seem to be a component of the
CC extracellular matrix of the aggregates. {ECO:0000269|PubMed:16008582}.
CC -!- MISCELLANEOUS: As bacterial aggregation is one of the initial steps of
CC biofilm formation, the clumping phenotype observed in the vjbR mutant
CC suggests that B.melitensis might be able to form biofilms.
CC {ECO:0000269|PubMed:17557825}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL54358.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE008918; AAL54358.1; ALT_INIT; Genomic_DNA.
DR PIR; AC3649; AC3649.
DR RefSeq; WP_002966460.1; NZ_GG703779.1.
DR AlphaFoldDB; Q8YAY5; -.
DR SMR; Q8YAY5; -.
DR STRING; 224914.BMEII1116; -.
DR EnsemblBacteria; AAL54358; AAL54358; BMEII1116.
DR GeneID; 45053211; -.
DR GeneID; 55591850; -.
DR KEGG; bme:BMEII1116; -.
DR PATRIC; fig|224914.52.peg.2242; -.
DR eggNOG; COG2771; Bacteria.
DR PhylomeDB; Q8YAY5; -.
DR PRO; PR:Q8YAY5; -.
DR Proteomes; UP000000419; Chromosome II.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0009372; P:quorum sensing; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR CDD; cd06170; LuxR_C_like; 1.
DR Gene3D; 1.10.10.10; -; 1.
DR Gene3D; 3.30.450.80; -; 1.
DR InterPro; IPR016032; Sig_transdc_resp-reg_C-effctor.
DR InterPro; IPR005143; TF_LuxR_autoind-bd_dom.
DR InterPro; IPR036693; TF_LuxR_autoind-bd_dom_sf.
DR InterPro; IPR000792; Tscrpt_reg_LuxR_C.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF03472; Autoind_bind; 1.
DR Pfam; PF00196; GerE; 1.
DR PRINTS; PR00038; HTHLUXR.
DR SMART; SM00421; HTH_LUXR; 1.
DR SUPFAM; SSF46894; SSF46894; 1.
DR SUPFAM; SSF75516; SSF75516; 1.
DR PROSITE; PS50043; HTH_LUXR_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Quorum sensing; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..259
FT /note="HTH-type quorum sensing-dependent transcriptional
FT regulator VjbR"
FT /id="PRO_0000319927"
FT DOMAIN 183..248
FT /note="HTH luxR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT DNA_BIND 207..226
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00411"
FT REGION 76..179
FT /note="C12-HSL binding"
FT /evidence="ECO:0000255"
FT MUTAGEN 82
FT /note="D->A: Behaves like a constitutive regulator, forms
FT cell aggregates."
FT /evidence="ECO:0000269|PubMed:17557825"
SQ SEQUENCE 259 AA; 28625 MW; 2D845BA0737094CC CRC64;
MSLDLVHFPN YKKTFFGSSF QSDTLALLTR IRDEIGCRYV THTYRGRVGD CTKVNSADLT
VLMTLPATWV ARYSSKNYFA IDPVFQEDAP YYRNDTSAIA RDLKEDADIC PAVAELLHDA
EKHGLGNLFI AVSARNPKGV AGCTVFTFEV EDEDRTQFLA RMRPRLLSLA GIIHGTVCGC
KDANSVASLL TPREVDCLRW AANGKTDGEI AEILSIARWT VVTYLQNAKI KLNCSNRTSA
VATALSLGII DMPEVQHLV
