CALBP_ENTHI
ID CALBP_ENTHI Reviewed; 134 AA.
AC P38505;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Calcium-binding protein;
DE Short=CABP;
OS Entamoeba histolytica.
OC Eukaryota; Amoebozoa; Evosea; Archamoebae; Mastigamoebida; Entamoebidae;
OC Entamoeba.
OX NCBI_TaxID=5759;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ATCC 30459 / HM-1:IMSS;
RX PubMed=1625702; DOI=10.1016/0166-6851(92)90044-k;
RA Prasad J., Bhattacharya S., Bhattacharya A.;
RT "Cloning and sequence analysis of a calcium-binding protein gene from a
RT pathogenic strain of Entamoeba histolytica.";
RL Mol. Biochem. Parasitol. 52:137-140(1992).
RN [2]
RP CHARACTERIZATION.
RX PubMed=8220585;
RA Prasad J., Bhattacharya S., Bhattacharya A.;
RT "The calcium binding protein of Entamoeba histolytica: expression in
RT Escherichia coli and immunochemical characterization.";
RL Cell. Mol. Biol. Res. 39:167-175(1993).
RN [3]
RP STRUCTURE BY NMR, AND SEQUENCE REVISION TO 131.
RX PubMed=11724551; DOI=10.1021/bi0114978;
RA Atreya H.S., Sahu S.C., Bhattacharya A., Chary K.V., Govil G.;
RT "NMR derived solution structure of an EF-hand calcium-binding protein from
RT Entamoeba histolytica.";
RL Biochemistry 40:14392-14403(2001).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH CALCIUM IONS, AND
RP SUBUNIT.
RX PubMed=17554780; DOI=10.1002/prot.21455;
RA Kumar S., Padhan N., Alam N., Gourinath S.;
RT "Crystal structure of calcium binding protein-1 from Entamoeba histolytica:
RT a novel arrangement of EF hand motifs.";
RL Proteins 68:990-998(2007).
CC -!- FUNCTION: Could play a role in the transduction of secondary messages
CC on binding of calcium.
CC -!- SUBUNIT: Trimer. {ECO:0000269|PubMed:17554780}.
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DR EMBL; M84155; AAA29089.1; -; mRNA.
DR PDB; 1JFJ; NMR; -; A=1-134.
DR PDB; 1JFK; NMR; -; A=1-134.
DR PDB; 2I18; NMR; -; A=71-134.
DR PDB; 2M7K; NMR; -; A=1-66.
DR PDB; 2M7M; NMR; -; A=1-66.
DR PDB; 2M7N; NMR; -; A=1-134.
DR PDB; 2NXQ; X-ray; 2.40 A; A/B=1-134.
DR PDB; 3LI6; X-ray; 2.50 A; A/D/G/J=1-66.
DR PDB; 3PX1; X-ray; 3.00 A; A/B=1-134.
DR PDB; 3QJK; X-ray; 3.00 A; A/B=1-134.
DR PDB; 3ULG; X-ray; 3.20 A; A/B=1-134.
DR PDBsum; 1JFJ; -.
DR PDBsum; 1JFK; -.
DR PDBsum; 2I18; -.
DR PDBsum; 2M7K; -.
DR PDBsum; 2M7M; -.
DR PDBsum; 2M7N; -.
DR PDBsum; 2NXQ; -.
DR PDBsum; 3LI6; -.
DR PDBsum; 3PX1; -.
DR PDBsum; 3QJK; -.
DR PDBsum; 3ULG; -.
DR AlphaFoldDB; P38505; -.
DR BMRB; P38505; -.
DR SMR; P38505; -.
DR STRING; 5759.rna_EHI_120900-1; -.
DR VEuPathDB; AmoebaDB:EHI5A_054650; -.
DR VEuPathDB; AmoebaDB:EHI7A_028430; -.
DR VEuPathDB; AmoebaDB:EHI8A_025670; -.
DR VEuPathDB; AmoebaDB:EHI_120900; -.
DR VEuPathDB; AmoebaDB:KM1_062600; -.
DR eggNOG; KOG0027; Eukaryota.
DR OMA; RFDSNKD; -.
DR EvolutionaryTrace; P38505; -.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 4.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Metal-binding; Repeat.
FT CHAIN 1..134
FT /note="Calcium-binding protein"
FT /id="PRO_0000073470"
FT DOMAIN 1..32
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 33..68
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 72..107
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 108..134
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 10
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 12
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 14
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 21
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 46
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 48
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 50
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 52
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 57
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 85
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 87
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 89
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 91
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 96
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 117
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 119
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 121
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 123
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 128
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /ligand_label="4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT HELIX 3..9
FT /evidence="ECO:0007829|PDB:2NXQ"
FT STRAND 14..16
FT /evidence="ECO:0007829|PDB:3LI6"
FT HELIX 19..45
FT /evidence="ECO:0007829|PDB:2NXQ"
FT STRAND 50..52
FT /evidence="ECO:0007829|PDB:2NXQ"
FT HELIX 55..61
FT /evidence="ECO:0007829|PDB:2NXQ"
FT TURN 62..65
FT /evidence="ECO:0007829|PDB:2NXQ"
FT HELIX 71..84
FT /evidence="ECO:0007829|PDB:1JFJ"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:1JFJ"
FT HELIX 94..101
FT /evidence="ECO:0007829|PDB:1JFJ"
FT TURN 102..104
FT /evidence="ECO:0007829|PDB:1JFJ"
FT HELIX 107..117
FT /evidence="ECO:0007829|PDB:1JFJ"
FT STRAND 119..125
FT /evidence="ECO:0007829|PDB:1JFJ"
FT HELIX 126..133
FT /evidence="ECO:0007829|PDB:1JFJ"
SQ SEQUENCE 134 AA; 14953 MW; 27C8678ED3563B02 CRC64;
MAEALFKEID VNGDGAVSYE EVKAFVSKKR AIKNEQLLQL IFKSIDADGN GEIDQNEFAK
FYGSIQGQDL SDDKIGLKVL YKLMDVDGDG KLTKEEVTSF FKKHGIEKVA EQVMKADANG
DGYITLEEFL EFSL
